LOCUS       pJ2048.2_Gs             5853 bp ds-DNA     circular     28-FEB-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    720..734
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    351..356
                     /label="SacI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    533..562
                     /label="Linker_1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1123..1137
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2411..2445
                     /label="P(BBa_J23150)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    315..320
                     /label="EcoRI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    777..866
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1088..1122
                     /label="P(BBa_J23100)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1138..1167
                     /label="Linker_24"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2491..2505
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            complement(4975..5853)
                     /label="araC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2750..2755
                     /label="AatII"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    578..597
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2914..2920
                     /label="BbvCI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    867..1066
                     /label="Spacer 1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2715..2734
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1292..1325
                     /label="RBS"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    1206..1220
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    498..517
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2544..2573
                     /label="Linker_17"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    700..719
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    518..532
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    357..371
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2396..2410
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2921..2928
                     /label="AscI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2609..2714
                     /label="sgRNA-4"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    563..577
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2040..2105
                     /label="MarAn20"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2106..2120
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2390..2395
                     /label="SalI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2851..2895
                     /label="Linker_8"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1073..1087
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    392..497
                     /label="sgRNA-3"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2190..2389
                     /label="Spacer 2.5"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2461..2490
                     /label="Linker_25"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2902..2913
                     /label="Spacer 15"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        274..302
                     /label="P(BAD) promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2794..2850
                     /label="ECK120033737 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1227..1256
                     /label="Linker_10"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(1183..1205)
                     /label="bs-4"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2756..2785
                     /label="Linker_11"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2896..2901
                     /label="KasI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2735..2749
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    771..776
                     /label="NcoI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2121..2128
                     /label="SbfI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    598..699
                     /label="sgRNA-4t4"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    735..740
                     /label="XhoI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2529..2543
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    321..350
                     /label="Linker_14"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2446..2460
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            1326..2039
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     oriT            4189..4824
                     /label="ColA ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2129..2189
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2574..2588
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2786..2793
                     /label="NotI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1221..1226
                     /label="BamHI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2589..2608
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1257..1271
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    741..770
                     /label="Linker_0"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1272..1291
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2506..2528)
                     /label="bs-3"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     gene            complement(3209..4066)
                     /label="AmpR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1168..1182
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1067..1072
                     /label="HindIII"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    372..391
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 ACTTTTCATA CTCCCGCCAT TCAGAGAAGA AACCAATTGT CCATATTGCA TCAGACATTG
       61 CCGTCACTGC GTCTTTTACT GGCTCTTCTC GCTAACCAAA CCGGTAACCC CGCTTATTAA
      121 AAGCATTCTG TAACAAAGCG GGACCAAAGC CATGACAAAA ACGCGTAACA AAAGTGTCTA
      181 TAATCACGGC AGAAAAGTCC ACATTGATTA TTTGCACGGC GTCACACTTT GCTATGCCAT
      241 AGCATTTTTA TCCATAAGAT TAGCGGtTCC TACCTGACGC TTTTTATCGC AACTCTCTAC
      301 TGTTTCTCCA TACCGAATTC ATAGGATAGA TTCTGGAAAC TTTACCGTCC GAGCTCCAGC
      361 CTGCGGTCCG GGTTCACTGC CGTATAGGCA GTGACTGAGC TAGTGTACTC TGTTTcAGAG
      421 CTATGCTGGA AACAGCATAG CAAGTTgAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG
      481 TGGCACCGAG TCGGTGCGTT CACTGCCGTA TAGGCAGTCG CTGGGACGCC CGGGGACTAC
      541 ACTTACGAAA CTATTGATTG CTCAGCCTGC GGTCCGGGTT CACTGCCGTA TAGGCAGCAT
      601 CTTAGAGTAT GTAGTTTcAG AGCTATGCTG GAAACAGCAT AGCAAGTTgA AATAAGGCTA
      661 GTCCGTTATC AACTTGAAAA AGTGGCACCG AGTCGGTGCG TTCACTGCCG TATAGGCAGT
      721 CGCTGGGACG CCCGCTCGAG CAATAAACAG TTGATAGGGC TTCTCCGTTA CCATGGTTCA
      781 GCCAAAAAAC TTAAGACCGC CGGTCTTGTC CACTACCTTG CAGTAATGCG GTGGACAGGA
      841 TCGGCGGTTT TCTTTTCTCT TCTCAATTCT TCTGACCTGT AACGAATAAT AGATAGTAAA
      901 GTAGTCTCCG ATTGAGTTTT CTCTGCCGAG TCCCACCCAG TTCTGTGATT TCAGTAAGTT
      961 GGTAATTGAT ACACTGTTGC GAGAACTGCT GCCTGGTAGT AGATAGGTTG TTATTGAGTA
     1021 AGAAGGTAAA GTGAACGAAA TCCCTGAAAC TGAGACTGTA GAAAATAAGC TTCAGCCTGC
     1081 GGTCCGGttg acggctagct cagtcctagg tacagtgcta gcTCGCTGGG ACGCCCGAGA
     1141 TAGCCGTTAC ACAGGTGACA CTTATTTCAG CCTGCGGTCC GGccaTACAT ACTCTAAGAT
     1201 GTGTCTCGCT GGGACGCCCG GGATCCAAGA GATTTCTACA CGATTGAGCA CTGTCTCAGC
     1261 CTGCGGTCCG GGTTCACTGC CGTATAGGCA GTAATTTTGT TTAACTTTAA GAAGGAGATA
     1321 TACATATGCG TAAAGGCGAA GAACTGTTTA CCGGTGTGGT TCCGATTCTG GTGGAACTGG
     1381 ACGGCGATGT TAATGGTCAT AAATTCAGTG TTCGCGGCGA AGGTGAAGGC GATGCGACGA
     1441 ACGGCAAACT GACCCTGAAA TTTATCTGCA CCACGGGTAA ACTGCCGGTC CCGTGGCCGA
     1501 CGCTGGTGAC CACGCTGACC TATGGCGTTC AATGTTTTGC GCGTTACCCG GATCACATGA
     1561 AACAGCACGA CTTTTTCAAA TCGGCCATGC CGGAAGGCTA TGTGCAGGAA CGTACGATTA
     1621 GCTTTAAAGA CGATGGTACG TATAAAACCC GCGCGGAAGT GAAATTCGAA GGCGATACCC
     1681 TGGTTAACCG TATCGAACTG AAAGGTATCG ATTTCAAAGA AGACGGCAAT ATTCTGGGTC
     1741 ATAAACTGGA ATATAACTTC AATTCCCACA ACGTGTACAT CACCGCGGAT AAACAGAAAA
     1801 ACGGCATTAA AGCCAATTTC AAAATCCGCC ATAATGTGGA AGATGGTAGC GTTCAGCTGG
     1861 CCGACCACTA TCAGCAAAAC ACGCCGATTG GTGATGGCCC GGTCCTGCTG CCGGACAATC
     1921 ACTACCTGAG TACCCAGTCC GTGCTGTCAA AAGATCCGAA CGAAAAACGT GACCACATGG
     1981 TCCTGCTGGA ATTTGTGACG GCTGCGGGTA TCACCCACGG CATGGACGAA CTGTATAAAA
     2041 TGTCCCGCCG TAATACTGAC GCCATCACAA TCCACAGCAT CCTGGATTGG ATTGAAGACT
     2101 AATAATCGCT GGGACGCCCG CCTGCAGGCT CGGTACCAAA TTCCAGAAAA GAGGCCTCCC
     2161 GAAAGGGGGG CCTTTTTTCG TTTTGGTCCT AATAGATAAA GGATAGGTCT GGTAGTGTTG
     2221 TTCGTTCTCG CAGGTAAATC AATAATACTC AGCAGTTCCG TAGACTTTTC AGTGGGACAG
     2281 GGTAGCGATA ACAGATAGAT TGTAATAAGA CACAGTAGGT GCTCGTAGTT GCGTGAAGAG
     2341 AACCGCTCAG GAAATCCAGT CAGAAGTATT GGTAATCGTT GAAAACTCAG TCGACCAGCC
     2401 TGCGGTCCGG TTTACGGCTA GCTCAGTCCT AGGTATTATG CTAGCTCGCT GGGACGCCCG
     2461 AGTGACGACT GCGAAGTAAC CTCTATTTAT CAGCCTGCGG TCCGGccaAG AGTACACTAG
     2521 CTCAGTCATC GCTGGGACGC CCGGTAGTGC TTATCAGACC CAATACTGTT GAACAGCCTG
     2581 CGGTCCGGGT TCACTGCCGT ATAGGCAGGA CACATCTTAG AGTATGTAGT TTcAGAGCTA
     2641 TGCTGGAAAC AGCATAGCAA GTTgAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG
     2701 CACCGAGTCG GTGCGTTCAC TGCCGTATAG GCAGTCGCTG GGACGCCCGG ACGTCCTATT
     2761 ACACTCGTCG TTGGAAACTG AAGATGCGGC CGCggaaaca cagAAAAAAG CCCGCACCTG
     2821 ACAGTGCGGG CTTTTTTTTT cgaccaaagg TAGCGAACGA CGAGTCACTG TTGAGGATAA
     2881 ATACTTTCTC TACTAGGCGC CTGTTACACA GGTCCTCAGC GGCGCGCCTT TGTCGGTGAA
     2941 CGCTCTCCTG AGTAGGACAA ATCCGCCGGG AGCGGATTTG AACGTTGTGA AGCAACGGCC
     3001 CGGAGGGTGG CGGGCAGGAC GCCCGCCATA AACTGCCAGG CATCAAACTA AGCAGAAGGC
     3061 CATCCTGACG GATGGCCTTT TTGCGTTTCA GATCTgggat tttggtcatg agattatcaa
     3121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     3541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     3841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4141 tttagaaaaa taaacaaata ggggttccgc gcagcatgct agcGgcagaa acgtcctaga
     4201 agatgccagg aggatactta gcagagagac aataaggccg gagcgaagcc gtttttccat
     4261 aggctccgcc cccctgacga acatcacgaa atctgacgct caaatcagtg gtggcgaaac
     4321 ccgacaggac tataaagata ccaggcgttt ccccctgatg gctccctctt gcgctctcct
     4381 gttcccgtcc tgcggcgtcc gtgttgtggt ggaggcttta cccaaatcac cacgtcccgt
     4441 tccgtgtaga cagttcgctc caagctgggc tgtgtgcaag aaccccccgt tcagcccgac
     4501 tgctgcgcct tatccggtaa ctatcatctt gagtccaacc cggaaagaca cgacaaaacg
     4561 ccactggcag cagccattgg taactgagaa ttagtggatt tagatatcga gagtcttgaa
     4621 gtggtggcct aacagaggct acactgaaag gacagtattt ggtatctgcg ctccactaaa
     4681 gccagttacc aggttaagca gttccccaac tgacttaacc ttcgatcaaa ccgcctcccc
     4741 aggcggtttt ttcgtttaca gagcaggaga ttacgacgat cgtaaaagga tctcaagaag
     4801 atcctttacg gattcccgac accatcactc tagatttcag tgcaatttat ctcttcaaat
     4861 gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgtta gtcatgcccc
     4921 gcgcccaccg gaaggagctg actgggttgC TCCTAgGGTC TGATTCGTTA CCAATTATGA
     4981 CAACTTGACG GCTACATCAT TCACTTTTTC TTCACAACCG GCACGGAACT CGCTCGGGCT
     5041 GGCCCCGGTG CATTTTTTAA ATACCCGCGA GAAATAGAGT TGATCGTCAA AACCAACATT
     5101 GCGACCGACG GTGGCGATAG GCATCCGGGT GGTGCTCAAA AGCAGCTTCG CCTGGCTGAT
     5161 ACGTTGGTCC TCGCGCCAGC TTAAGACGCT AATCCCTAAC TGCTGGCGGA AAAGATGTGA
     5221 CAGACGCGAC GGCGACAAGC AAACATGCTG TGCGACGCTG GCGATATCAA AATTGCTGTC
     5281 TGCCAGGTGA TCGCTGATGT ACTGACAAGC CTCGCGTACC CGATTATCCA TCGGTGGATG
     5341 GAGCGACTCG TTAATCGCTT CCATGCGCCG CAGTAACAAT TGCTCAAGCA GATTTATCGC
     5401 CAGCAGCTCC GAATAGCGCC CTTCCCCTTG CCCGGCGTTA ATGATTTGCC CAAACAGGTC
     5461 GCTGAAATGC GGCTGGTGCG CTTCATCCGG GCGAAAGAAC CCCGTATTGG CAAATATTGA
     5521 CGGCCAGTTA AGCCATTCAT GCCAGTAGGC GCGCGGACGA AAGTAAACCC ACTGGTGATA
     5581 CCATTCGCGA GCCTCCGGAT GACGACCGTA GTGATGAATC TCTCCTGGCG GGAACAGCAA
     5641 AATATCACCC GGTCGGCAAA CAAATTCTCG TCCCTGATTT TTCACCACCC CCTGACCGCG
     5701 AATGGTGAGA TTGAGAATAT AACCTTTCAT TCCCAGCGGT CGGTCGATAA AAAAATCGAG
     5761 ATAACCGTTG GCCTCAATCG GCGTTAAACC CGCCACCAGA TGGGCATTAA ACGAGTATCC
     5821 CGGCAGCAGG GGATCATTTT GCGCTTCAGC CAT
//