LOCUS pJ2042.2_GFPonly 5858 bp ds-DNA circular 21-FEB-2020 DEFINITION . FEATURES Location/Qualifiers misc_feature complement(1183..1205) /label="bs-1" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 1168..1182 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2794..2850 /label="ECK120033737 Terminator" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac promoter 274..302 /label="P(BAD) promoter" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 2461..2490 /label="Linker_25" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 514..528 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 gene complement(4980..5858) /label="araC" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 315..320 /label="EcoRI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 2851..2895 /label="Linker_8" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 321..350 /label="Linker_14" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 2914..2920 /label="BbvCI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 351..356 /label="SacI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 1138..1167 /label="Linker_24" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 2190..2389 /label="Spacer 2.5" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 2902..2913 /label="Spacer 15" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 2896..2901 /label="KasI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 494..513 /label="Csy4 site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 529..558 /label="Linker_1" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 1088..1122 /label="P(BBa_J23100)" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 735..740 /label="XhoI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 1206..1220 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2106..2120 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 574..593 /label="Csy4 site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 867..1066 /label="Spacer 1" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 2411..2445 /label="P(BBa_J23150)" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 gene 1326..2039 /label="sfGFP" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2735..2749 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2396..2410 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 594..699 /label="sgRNA-2" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 1123..1137 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1073..1087 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2609..2714 /label="sgRNA-1" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 2390..2395 /label="SalI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 1221..1226 /label="BamHI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 2544..2573 /label="Linker_17" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 372..391 /label="Csy4 site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 2529..2543 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1292..1325 /label="RBS" /ApEinfo_revcolor=#7ea7d8 /ApEinfo_fwdcolor=#7ea7d8 misc_feature 700..719 /label="Csy4 site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1257..1271 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 771..776 /label="NcoI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 720..734 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2750..2755 /label="AatII" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 2921..2928 /label="AscI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 392..493 /label="sgRNA-1t4" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 777..866 /label="ECK120029600 Terminator" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 2786..2793 /label="NotI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 2756..2785 /label="Linker_11" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 357..371 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1067..1072 /label="HindIII" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 2121..2128 /label="SbfI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 741..770 /label="Linker_0" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 oriT 4194..4829 /label="ColA ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 559..573 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2491..2505 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 gene complement(3269..4084) /label="KanR" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 2129..2189 /label="L3S2P21 Terminator" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature complement(2506..2528) /label="bs-2" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 2574..2588 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2446..2460 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1272..1291 /label="Csy4 site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 2040..2105 /label="MarAn20" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 2589..2608 /label="Csy4 site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1227..1256 /label="Linker_10" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 2715..2734 /label="Csy4 site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 ORIGIN 1 ACTTTTCATA CTCCCGCCAT TCAGAGAAGA AACCAATTGT CCATATTGCA TCAGACATTG 61 CCGTCACTGC GTCTTTTACT GGCTCTTCTC GCTAACCAAA CCGGTAACCC CGCTTATTAA 121 AAGCATTCTG TAACAAAGCG GGACCAAAGC CATGACAAAA ACGCGTAACA AAAGTGTCTA 181 TAATCACGGC AGAAAAGTCC ACATTGATTA TTTGCACGGC GTCACACTTT GCTATGCCAT 241 AGCATTTTTA TCCATAAGAT TAGCGGtTCC TACCTGACGC TTTTTATCGC AACTCTCTAC 301 TGTTTCTCCA TACCGAATTC ATAGGATAGA TTCTGGAAAC TTTACCGTCC GAGCTCCAGC 361 CTGCGGTCCG GGTTCACTGC CGTATAGGCA GAAGCTAGAC TCTAGTGGTT TcAGAGCTAT 421 GCTGGAAACA GCATAGCAAG TTgAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC 481 ACCGAGTCGG TGCGTTCACT GCCGTATAGG CAGTCGCTGG GACGCCCGGG GACTACACTT 541 ACGAAACTAT TGATTGCTCA GCCTGCGGTC CGGGTTCACT GCCGTATAGG CAGATCAGTG 601 TGTACTAAGT ACTGTTTcAG AGCTATGCTG GAAACAGCAT AGCAAGTTgA AATAAGGCTA 661 GTCCGTTATC AACTTGAAAA AGTGGCACCG AGTCGGTGCG TTCACTGCCG TATAGGCAGT 721 CGCTGGGACG CCCGCTCGAG CAATAAACAG TTGATAGGGC TTCTCCGTTA CCATGGTTCA 781 GCCAAAAAAC TTAAGACCGC CGGTCTTGTC CACTACCTTG CAGTAATGCG GTGGACAGGA 841 TCGGCGGTTT TCTTTTCTCT TCTCAATTCT TCTGACCTGT AACGAATAAT AGATAGTAAA 901 GTAGTCTCCG ATTGAGTTTT CTCTGCCGAG TCCCACCCAG TTCTGTGATT TCAGTAAGTT 961 GGTAATTGAT ACACTGTTGC GAGAACTGCT GCCTGGTAGT AGATAGGTTG TTATTGAGTA 1021 AGAAGGTAAA GTGAACGAAA TCCCTGAAAC TGAGACTGTA GAAAATAAGC TTCAGCCTGC 1081 GGTCCGGttg acggctagct cagtcctagg tacagtgcta gcTCGCTGGG ACGCCCGAGA 1141 TAGCCGTTAC ACAGGTGACA CTTATTTCAG CCTGCGGTCC GGccaCACTA GAGTCTAGCT 1201 TGAGATCGCT GGGACGCCCG GGATCCAAGA GATTTCTACA CGATTGAGCA CTGTCTCAGC 1261 CTGCGGTCCG GGTTCACTGC CGTATAGGCA GTAATTTTGT TTAACTTTAA GAAGGAGATA 1321 TACATATGCG TAAAGGCGAA GAACTGTTTA CCGGTGTGGT TCCGATTCTG GTGGAACTGG 1381 ACGGCGATGT TAATGGTCAT AAATTCAGTG TTCGCGGCGA AGGTGAAGGC GATGCGACGA 1441 ACGGCAAACT GACCCTGAAA TTTATCTGCA CCACGGGTAA ACTGCCGGTC CCGTGGCCGA 1501 CGCTGGTGAC CACGCTGACC TATGGCGTTC AATGTTTTGC GCGTTACCCG GATCACATGA 1561 AACAGCACGA CTTTTTCAAA TCGGCCATGC CGGAAGGCTA TGTGCAGGAA CGTACGATTA 1621 GCTTTAAAGA CGATGGTACG TATAAAACCC GCGCGGAAGT GAAATTCGAA GGCGATACCC 1681 TGGTTAACCG TATCGAACTG AAAGGTATCG ATTTCAAAGA AGACGGCAAT ATTCTGGGTC 1741 ATAAACTGGA ATATAACTTC AATTCCCACA ACGTGTACAT CACCGCGGAT AAACAGAAAA 1801 ACGGCATTAA AGCCAATTTC AAAATCCGCC ATAATGTGGA AGATGGTAGC GTTCAGCTGG 1861 CCGACCACTA TCAGCAAAAC ACGCCGATTG GTGATGGCCC GGTCCTGCTG CCGGACAATC 1921 ACTACCTGAG TACCCAGTCC GTGCTGTCAA AAGATCCGAA CGAAAAACGT GACCACATGG 1981 TCCTGCTGGA ATTTGTGACG GCTGCGGGTA TCACCCACGG CATGGACGAA CTGTATAAAA 2041 TGTCCCGCCG TAATACTGAC GCCATCACAA TCCACAGCAT CCTGGATTGG ATTGAAGACT 2101 AATAATCGCT GGGACGCCCG CCTGCAGGCT CGGTACCAAA TTCCAGAAAA GAGGCCTCCC 2161 GAAAGGGGGG CCTTTTTTCG TTTTGGTCCT AATAGATAAA GGATAGGTCT GGTAGTGTTG 2221 TTCGTTCTCG CAGGTAAATC AATAATACTC AGCAGTTCCG TAGACTTTTC AGTGGGACAG 2281 GGTAGCGATA ACAGATAGAT TGTAATAAGA CACAGTAGGT GCTCGTAGTT GCGTGAAGAG 2341 AACCGCTCAG GAAATCCAGT CAGAAGTATT GGTAATCGTT GAAAACTCAG TCGACCAGCC 2401 TGCGGTCCGG TTTACGGCTA GCTCAGTCCT AGGTATTATG CTAGCTCGCT GGGACGCCCG 2461 AGTGACGACT GCGAAGTAAC CTCTATTTAT CAGCCTGCGG TCCGGccaAG TACTTAGTAC 2521 ACACTGATTC GCTGGGACGC CCGGTAGTGC TTATCAGACC CAATACTGTT GAACAGCCTG 2581 CGGTCCGGGT TCACTGCCGT ATAGGCAGTC TCAAGCTAGA CTCTAGTGGT TTcAGAGCTA 2641 TGCTGGAAAC AGCATAGCAA GTTgAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG 2701 CACCGAGTCG GTGCGTTCAC TGCCGTATAG GCAGTCGCTG GGACGCCCGG ACGTCCTATT 2761 ACACTCGTCG TTGGAAACTG AAGATGCGGC CGCggaaaca cagAAAAAAG CCCGCACCTG 2821 ACAGTGCGGG CTTTTTTTTT cgaccaaagg TAGCGAACGA CGAGTCACTG TTGAGGATAA 2881 ATACTTTCTC TACTAGGCGC CTGTTACACA GGTCCTCAGC GGCGCGCCTT TGTCGGTGAA 2941 CGCTCTCCTG AGTAGGACAA ATCCGCCGGG AGCGGATTTG AACGTTGTGA AGCAACGGCC 3001 CGGAGGGTGG CGGGCAGGAC GCCCGCCATA AACTGCCAGG CATCAAACTA AGCAGAAGGC 3061 CATCCTGACG GATGGCCTTT TTGCGTTTCA GATCTACCGG Taaaccagca atagacataa 3121 gcggctattt aacgaccctg ccctgaaccg acgacaagct gacgaccggg tctccgcaag 3181 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 3241 aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact 3301 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg 3361 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga 3421 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat 3481 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca 3541 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat 3601 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cggtcgctgt 3661 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat 3721 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg 3781 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg 3841 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg 3901 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc 3961 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat 4021 cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc 4081 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 4141 gatacatatt tgaatgtatt tagaaaaata aacaaatagg catgctagcg cagaaacgtc 4201 ctagaagatg ccaggaggat acttagcaga gagacaataa ggccggagcg aagccgtttt 4261 tccataggct ccgcccccct gacgaacatc acgaaatctg acgctcaaat cagtggtggc 4321 gaaacccgac aggactataa agataccagg cgtttccccc tgatggctcc ctcttgcgct 4381 ctcctgttcc cgtcctgcgg cgtccgtgtt gtggtggagg ctttacccaa atcaccacgt 4441 cccgttccgt gtagacagtt cgctccaagc tgggctgtgt gcaagaaccc cccgttcagc 4501 ccgactgctg cgccttatcc ggtaactatc atcttgagtc caacccggaa agacacgaca 4561 aaacgccact ggcagcagcc attggtaact gagaattagt ggatttagat atcgagagtc 4621 ttgaagtggt ggcctaacag aggctacact gaaaggacag tatttggtat ctgcgctcca 4681 ctaaagccag ttaccaggtt aagcagttcc ccaactgact taaccttcga tcaaaccgcc 4741 tccccaggcg gttttttcgt ttacagagca ggagattacg acgatcgtaa aaggatctca 4801 agaagatcct ttacggattc ccgacaccat cactctagat ttcagtgcaa tttatctctt 4861 caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat 4921 gccccgcgcc caccggaagg agctgactgg gttgCTCCTA gGGTCTGATT CGTTACCAAT 4981 TATGACAACT TGACGGCTAC ATCATTCACT TTTTCTTCAC AACCGGCACG GAACTCGCTC 5041 GGGCTGGCCC CGGTGCATTT TTTAAATACC CGCGAGAAAT AGAGTTGATC GTCAAAACCA 5101 ACATTGCGAC CGACGGTGGC GATAGGCATC CGGGTGGTGC TCAAAAGCAG CTTCGCCTGG 5161 CTGATACGTT GGTCCTCGCG CCAGCTTAAG ACGCTAATCC CTAACTGCTG GCGGAAAAGA 5221 TGTGACAGAC GCGACGGCGA CAAGCAAACA TGCTGTGCGA CGCTGGCGAT ATCAAAATTG 5281 CTGTCTGCCA GGTGATCGCT GATGTACTGA CAAGCCTCGC GTACCCGATT ATCCATCGGT 5341 GGATGGAGCG ACTCGTTAAT CGCTTCCATG CGCCGCAGTA ACAATTGCTC AAGCAGATTT 5401 ATCGCCAGCA GCTCCGAATA GCGCCCTTCC CCTTGCCCGG CGTTAATGAT TTGCCCAAAC 5461 AGGTCGCTGA AATGCGGCTG GTGCGCTTCA TCCGGGCGAA AGAACCCCGT ATTGGCAAAT 5521 ATTGACGGCC AGTTAAGCCA TTCATGCCAG TAGGCGCGCG GACGAAAGTA AACCCACTGG 5581 TGATACCATT CGCGAGCCTC CGGATGACGA CCGTAGTGAT GAATCTCTCC TGGCGGGAAC 5641 AGCAAAATAT CACCCGGTCG GCAAACAAAT TCTCGTCCCT GATTTTTCAC CACCCCCTGA 5701 CCGCGAATGG TGAGATTGAG AATATAACCT TTCATTCCCA GCGGTCGGTC GATAAAAAAA 5761 TCGAGATAAC CGTTGGCCTC AATCGGCGTT AAACCCGCCA CCAGATGGGC ATTAAACGAG 5821 TATCCCGGCA GCAGGGGATC ATTTTGCGCT TCAGCCAT //