LOCUS       Sender_plasmid2         3860 bp ds-DNA     circular     26-FEB-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2489..3077
                     /label="PMB1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    506..571
                     /label="BCD"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1369..1463
                     /label="B0014terminator"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     misc_feature    444..448
                     /label="-35"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    479..499
                     /label="LacO"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(3507..3708)
                     /label="ROP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     sig_peptide     1191..1223
                     /label="ssrA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     gene            complement(1552..2367)
                     /label="Kan"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    467..472
                     /label="-10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    582..1184
                     /label="RhlI from PAO1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    449..466
                     /label="LacO"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    64..86
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 agtgcgCTCG AGcttcccaa ccttaccaga gggcgcccca gctggcaatt ccgacgtctg
       61 tgtggaattg tgagcggata acaatttcac acagggccct cggacaccga ggagaatgtc
      121 aagaggcgaa cacacaacgt cttggagcgc cagaggagga acgagctaaa acggagcttt
      181 tttgccctgc gtgaccagat cccggagttg gaaaacaatg aaaaggcccc caaggtagtt
      241 atccttaaaa aagccacagc atacatcctg tccgtccaag cagaggagca aaagctcatt
      301 tctgaagagg acttgttgcg gaaacgacga gaacagttga aacacaaact tgaacagcta
      361 cggaactctt gtgcgtaagg aaaagtaagg aaaacgattc cttctaacag aaatgtcctg
      421 agcaatcacc tatgaactgt cgaTTGACAT TGTGAGCGGA TAACAAGATA CTGAGCACAA
      481 TTGTGAGCGG ATAACAATTg catgccactt aaaaaggaga tcaacaatga aagcaatttt
      541 cgtactgaaa catcttaatc atgctaagga gaaatactag tatgatcgaa ttgctctctg
      601 aatcgctgga agggctttcc gccgccatga tcgccgagct gggacgctac cggcatcagg
      661 tcttcatcga gaagctgggc tgggacgtgg tctccacctc cagggtccgc gaccaggaat
      721 tcgaccagtt cgaccatccg caaacccgct acatcgtcgc catgagccgc cagggcatct
      781 gcggttgcgc ccgcctgctg ccgacgaccg acgcctacct gctcaaggac gtcttcgcct
      841 acctgtgcag cgaaaccccg ccgagcgatc cgtcggtctg ggagctttcg cgctacgccg
      901 ccagcgcggc ggacgatccg cagctggcga tgaagatatt ctggtccagc ctgcaatgcg
      961 cctggtacct gggcgccagt tcggtggtgg cggtgaccac cacggccatg gagcgctatt
     1021 tcgttcgcaa cggcgtgatc ctccagcgcc tcggcccgcc gcagaaggtc aagggcgaga
     1081 cgctggtcgc gatcagcttc ccggcctacc aggagcgcgg cctggagatg ctgctgcgct
     1141 accacccgga atggctgcag ggcgtaccgc tgtcgatggc ggtgacctcc gcagcgaacg
     1201 acgaaaatta cgcccttgca gcgtaataaA AGCTTcccct acgtgctgct gaagttgccc
     1261 gcaacagaga gtggaaccaa ccggtgatac cacgatacta tgactgagag tcaacgccat
     1321 gagcggcctc atttcttatt ctgagttaca acagtccgca ccgctgtctc acactggctc
     1381 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta
     1441 ttaatccggc ttttttatta tttcctaGCT AGCcggggaa atgtgcgcgg aacccctatt
     1501 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa
     1561 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
     1621 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
     1681 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
     1741 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
     1801 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
     1861 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
     1921 gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
     1981 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
     2041 tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt
     2101 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
     2161 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg
     2221 cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg
     2281 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca
     2341 agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga
     2401 cagttttatt gttcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2461 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2521 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2581 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     2641 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2701 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2761 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2821 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2881 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2941 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3001 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3061 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3121 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3181 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3241 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3301 tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3361 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     3421 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3481 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     3541 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
     3601 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
     3661 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
     3721 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt
     3781 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg
     3841 atgcggcggg accaGAGCTC
//