LOCUS       Receiver_plasmid2       3950 bp ds-DNA     circular     26-FEB-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2601..3189
                     /label="PMB1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    491..507
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    466..484
                     /label="R0071 pRhl from pseudomonas PAO1"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    1055..1057
                     /label="F145Y"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    89..105
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     gene            complement(1664..2479)
                     /label="Kan"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    1058..1060
                     /label="N146Y"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    485..490
                     /label="-35"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    547..612
                     /label="BCD"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1064..1066
                     /label="H148D"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(3619..3820)
                     /label="ROP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    521..539
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    508..513
                     /label="-10"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1481..1575
                     /label="B0014terminator"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     misc_feature    623..1341
                     /label="superfolder GFP->CFP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 ggatgcggcg ggaccaGAGC TCagtgcgCT CGAGcttccc aaccttacca gagggcgccc
       61 cagctggcaa ttccgacgtc tgtgtggatc cctatcagtg atagagattc acacagggcc
      121 ctcggacacc gaggagaatg tcaagaggcg aacacacaac gtcttggagc gccagaggag
      181 gaacgagcta aaacggagct tttttgccct gcgtgaccag atcccggagt tggaaaacaa
      241 tgaaaaggcc cccaaggtag ttatccttaa aaaagccaca gcatacatcc tgtccgtcca
      301 agcagaggag caaaagctca tttctgaaga ggacttgttg cggaaacgac gagaacagtt
      361 gaaacacaaa cttgaacagc tacggaactc ttgtgcgtaa ggaaaagtaa ggaaaacgat
      421 tccttctaac agaaatgtcc tgagcaatca cctatgaact gtcgatcctg tgaaatctgg
      481 cagtTTTACT tccctatcag tgatagaGAT ACTGAGCACa tccctatcag tgatagagat
      541 gcatgccact taaaaaggag atcaacaatg aaagcaattt tcgtactgaa acatcttaat
      601 catgctaagg agaaatacta gtatgcgtaa aggcgaagag ctgttcactg gtgtcgtccc
      661 tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg
      721 tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta ctggtaaact
      781 gccggtacct tggccgactc tggtaacgac gctgactTGG ggtgttcagt gctttgctcg
      841 ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg aaggctatgt
      901 gcaggaacgc acgatttcct ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa
      961 atttgaaggc gataccctgg taaaccgcat tgagctgaaa ggcattgact ttaaagaaga
     1021 cggcaatatc ctgggccata agctggaata caatTACATC agcGACaatg tttacatcac
     1081 cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca acgtggagga
     1141 tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg atggtcctgt
     1201 tctgctgcca gacaatcact atctgagcac gcaaagcgtt ctgtctaaag atccgaacga
     1261 gaaacgcgat catatggttc tgctggagtt cgtaaccgca gcgggcatca cgcatggtat
     1321 ggatgaactg tacaaatgat gAAGCTTccc ctacgtgctg ctgaagttgc ccgcaacaga
     1381 gagtggaacc aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc
     1441 tcatttctta ttctgagtta caacagtccg caccgctgtc tcacactggc tcaccttcgg
     1501 gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg
     1561 gcttttttat tatttcctaG CTAGCcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1621 tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga
     1681 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     1741 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     1801 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     1861 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     1921 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     1981 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     2041 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     2101 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     2161 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     2221 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     2281 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     2341 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     2401 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt
     2461 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     2521 ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2581 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2641 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2701 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2761 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2821 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2881 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2941 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3001 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3061 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3121 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3181 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3241 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3301 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3361 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3421 cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     3481 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     3541 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     3601 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     3661 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     3721 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     3781 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     3841 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     3901 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
//