LOCUS       FU\GFP-Pte  11183 bp    DNA   circular             2-MAR-2020
DEFINITION  Complementary copy of FUGW 2.1 rc.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|-13235641|
COMMENT     VNTDBDATE|-13235641|
COMMENT     LSOWNER|
COMMENT     VNTNAME|FU GFP-PtenD252G|
COMMENT     VNTAUTHORNAME|Hal|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     misc_feature    5874..6463
                     /vntifkey="21"
                     /label=WRE
     LTR             6479..7168
                     /vntifkey="19"
                     /label=Sin\LTR
     promoter        9372..10045
                     /vntifkey="30"
                     /label=pUC
     CDS             10190..11050
                     /vntifkey="4"
                     /label=AmpR
     promoter        212..816
                     /vntifkey="29"
                     /label=CMV
     LTR             835..1509
                     /vntifkey="19"
                     /label=5'LTR
     misc_feature    1533..2390
                     /vntifkey="21"
                     /label=Psi
     misc_feature    2748..2753
                     /vntifkey="21"
                     /label=SP1
     misc_feature    2932..2937
                     /vntifkey="21"
                     /label=SP1
     TATA_signal     2952..2958
                     /vntifkey="41"
                     /label=Ubi\TATA
     misc_feature    2983..2983
                     /vntifkey="21"
                     /label=TI
     intron          3047..3832
                     /vntifkey="15"
                     /label=Ubi\Intron\1
     misc_feature    2613..2628
                     /vntifkey="21"
                     /label=insert\U6
     misc_feature    3047..3048
                     /vntifkey="21"
                     /label=splice\donor
     misc_feature    3856..3857
                     /vntifkey="21"
                     /label=splice\acceptor
     misc_feature    3830..3855
                     /vntifkey="21"
                     /label=Pyr\Tract
     misc_feature    4630..4638
                     /vntifkey="21"
                     /label=linker
     CDS             4639..5847
                     /vntifkey="4"
                     /label=human\Pten
     CDS             3913..4629
                     /vntifkey="4"
                     /label=GFP
     misc_feature    5304..5316
                     /vntifkey="21"
                     /label=shMut
     misc_feature    5614..5616
                     /vntifkey="21"
                     /label=D326
     misc_feature    5458..5460
                     /vntifkey="21"
                     /label=W274
     misc_feature    5392..5394
                     /vntifkey="21"
                     /label=D252G
     misc_feature    5359..5361
                     /vntifkey="21"
                     /label=F241
     misc_feature    4915..4917
                     /vntifkey="21"
                     /label=H93
     misc_feature    4639..5193
                     /vntifkey="21"
                     /label=p-domain
     misc_feature    5194..5850
                     /vntifkey="21"
                     /label=c-term
     misc_feature    5692..5850
                     /vntifkey="21"
                     /label=c-tail
     misc_feature    5464..5466
                     /vntifkey="21"
                     /label=N276
     misc_feature    5269..5269
                     /vntifkey="21"
                     /label=Ezra\dupT
BASE COUNT     2818 a      2715 c      2945 g      2705 t 
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 
     2581 agatccagtt tggttaatcg catgctagct agttcgaacc ttaattaacc cgtgtcggct 
     2641 ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc 
     2701 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct 
     2761 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga 
     2821 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac 
     2881 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga 
     2941 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg 
     3001 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct 
     3061 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga 
     3121 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg 
     3181 ggggagcgca caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtaaggcgg 
     3241 gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg 
     3301 aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg 
     3361 ggaccctgac gtgaagtttg tcactgactg gagaactcgg gtttgtcgtc tggttgcggg 
     3421 ggcggcagtt atgcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgcctcg 
     3481 tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt 
     3541 aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct 
     3601 cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct 
     3661 ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg 
     3721 ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt 
     3781 tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt 
     3841 tttggctttt ttgttagacg aagcttgggc tgcaggtcga ctctagagga tccccgggta 
     3901 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
     3961 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
     4021 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 
     4081 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 
     4141 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
     4201 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
     4261 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     4321 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 
     4381 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 
     4441 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     4501 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 
     4561 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     4621 ctgtacaaga aaggatccat gacagccatc atcaaagaga tcgttagcag aaacaaaagg 
     4681 agatatcaag aggatggatt cgacttagac ttgacctata tttatccaaa cattattgct 
     4741 atgggatttc ctgcagaaag acttgaaggc gtatacagga acaatattga tgatgtagta 
     4801 aggtttttgg attcaaagca taaaaaccat tacaagatat acaatctttg tgctgaaaga 
     4861 cattatgaca ccgccaaatt taattgcaga gttgcacaat atccttttga agaccataac 
     4921 ccaccacagc tagaacttat caaacccttt tgtgaagatc ttgaccaatg gctaagtgaa 
     4981 gatgacaatc atgttgcagc aattcactgt aaagctggaa agggacgaac tggtgtaatg 
     5041 atatgtgcat atttattaca tcggggcaaa tttttaaagg cacaagaggc cctagatttc 
     5101 tatggggaag taaggaccag agacaaaaag ggagtaacta ttcccagtca gaggcgctat 
     5161 gtgtattatt atagctacct gttaaagaat catctggatt atagaccagt ggcactgttg 
     5221 tttcacaaga tgatgtttga aactattcca atgttcagtg gcggaacttg caatcctcag 
     5281 tttgtggtct gccagctaaa ggtcaaaatt tactcgtcca attcaggacc cacacgacgg 
     5341 gaagacaagt tcatgtactt tgagttccct cagccgttac ctgtgtgtgg tggtatcaaa 
     5401 gtagagttct tccacaaaca gaacaagatg ctaaaaaagg acaaaatgtt tcacttttgg 
     5461 gtaaatacat tcttcatacc aggaccagag gaaacctcag aaaaagtaga aaatggaagt 
     5521 ctatgtgatc aagaaatcga tagcatttgc agtatagagc gtgcagataa tgacaaggaa 
     5581 tatctagtac ttactttaac aaaaaatgat cttgacaaag caaataaaga caaagccaac 
     5641 cgatactttt ctccaaattt taaggtgaag ctgtacttca caaaaacagt agaggagccg 
     5701 tcaaatccag aggctagcag ttcaacttct gtaacaccag atgttagtga caatgaacct 
     5761 gatcattata gatattctga caccactgac tctgatccag agaatgaacc ttttgatgaa 
     5821 gatcagcata cacaaattac aaaagtctga gaattcgata tcaagcttat cgataatcaa 
     5881 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 
     5941 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 
     6001 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 
     6061 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 
     6121 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 
     6181 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 
     6241 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt 
     6301 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 
     6361 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 
     6421 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct 
     6481 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat 
     6541 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta 
     6601 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 
     6661 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc 
     6721 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga 
     6781 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa 
     6841 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat 
     6901 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg 
     6961 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg 
     7021 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 
     7081 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 
     7141 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac 
     7201 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 
     7261 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 
     7321 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 
     7381 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag 
     7441 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc 
     7501 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 
     7561 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 
     7621 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 
     7681 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 
     7741 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 
     7801 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 
     7861 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag 
     7921 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 
     7981 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 
     8041 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 
     8101 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 
     8161 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt 
     8221 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat 
     8281 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg 
     8341 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg 
     8401 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg 
     8461 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc 
     8521 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg 
     8581 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg 
     8641 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg 
     8701 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg 
     8761 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 
     8821 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 
     8881 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     8941 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 
     9001 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 
     9061 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 
     9121 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
     9181 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     9241 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     9301 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
     9361 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
     9421 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
     9481 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     9541 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     9601 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     9661 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     9721 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     9781 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     9841 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 
     9901 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     9961 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
    10021 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
    10081 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 
    10141 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 
    10201 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 
    10261 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 
    10321 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 
    10381 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 
    10441 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 
    10501 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 
    10561 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 
    10621 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 
    10681 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 
    10741 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 
    10801 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
    10861 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
    10921 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
    10981 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
    11041 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 
    11101 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 
    11161 ttccccgaaa agtgccacct gac 
//
