LOCUS       FU\GFP-T2A  11246 bp    DNA   circular            16-APR-2013
DEFINITION  Complementary copy of FUGW 2.1 rc.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|615835441|
COMMENT     VNTDBDATE|659470111|
COMMENT     LSOWNER|
COMMENT     VNTNAME|FU GFP-T2A-Pten|
COMMENT     VNTAUTHORNAME|Hal|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     misc_feature    5937..6526
                     /vntifkey="21"
                     /label=WRE
     LTR             6542..7231
                     /vntifkey="19"
                     /label=Sin\LTR
     promoter        9435..10108
                     /vntifkey="30"
                     /label=pUC
     CDS             10253..11113
                     /vntifkey="4"
                     /label=AmpR
     promoter        212..816
                     /vntifkey="29"
                     /label=CMV
     LTR             835..1509
                     /vntifkey="19"
                     /label=5'LTR
     misc_feature    1533..2390
                     /vntifkey="21"
                     /label=Psi
     misc_feature    2748..2753
                     /vntifkey="21"
                     /label=SP1
     misc_feature    2932..2937
                     /vntifkey="21"
                     /label=SP1
     TATA_signal     2952..2958
                     /vntifkey="41"
                     /label=Ubi\TATA
     misc_feature    2983..2983
                     /vntifkey="21"
                     /label=TI
     intron          3047..3832
                     /vntifkey="15"
                     /label=Ubi\Intron\1
     misc_feature    2613..2628
                     /vntifkey="21"
                     /label=insert\U6
     misc_feature    3047..3048
                     /vntifkey="21"
                     /label=splice\donor
     misc_feature    3856..3857
                     /vntifkey="21"
                     /label=splice\acceptor
     misc_feature    3830..3855
                     /vntifkey="21"
                     /label=Pyr\Tract
     misc_feature    4693..4701
                     /vntifkey="21"
                     /label=linker
     CDS             4702..5910
                     /vntifkey="4"
                     /label=human\Pten
     CDS             4630..4683
                     /vntifkey="4"
                     /label=T2A
     CDS             3913..4629
                     /vntifkey="4"
                     /label=GFP
     misc_feature    5367..5379
                     /vntifkey="21"
                     /label=shMut
     misc_feature    5455..5457
                     /vntifkey="21"
                     /label=D252G
BASE COUNT     2836 a      2728 c      2967 g      2715 t 
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 
     2581 agatccagtt tggttaatcg catgctagct agttcgaacc ttaattaacc cgtgtcggct 
     2641 ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc 
     2701 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct 
     2761 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga 
     2821 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac 
     2881 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga 
     2941 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg 
     3001 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct 
     3061 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga 
     3121 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg 
     3181 ggggagcgca caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtaaggcgg 
     3241 gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg 
     3301 aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg 
     3361 ggaccctgac gtgaagtttg tcactgactg gagaactcgg gtttgtcgtc tggttgcggg 
     3421 ggcggcagtt atgcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgcctcg 
     3481 tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt 
     3541 aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct 
     3601 cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct 
     3661 ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg 
     3721 ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt 
     3781 tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt 
     3841 tttggctttt ttgttagacg aagcttgggc tgcaggtcga ctctagagga tccccgggta 
     3901 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
     3961 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
     4021 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 
     4081 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 
     4141 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
     4201 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
     4261 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     4321 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 
     4381 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 
     4441 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     4501 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 
     4561 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     4621 ctgtacaagg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 
     4681 cctaagtaca agaaaggatc catgacagcc atcatcaaag agatcgttag cagaaacaaa 
     4741 aggagatatc aagaggatgg attcgactta gacttgacct atatttatcc aaacattatt 
     4801 gctatgggat ttcctgcaga aagacttgaa ggcgtataca ggaacaatat tgatgatgta 
     4861 gtaaggtttt tggattcaaa gcataaaaac cattacaaga tatacaatct ttgtgctgaa 
     4921 agacattatg acaccgccaa atttaattgc agagttgcac aatatccttt tgaagaccat 
     4981 aacccaccac agctagaact tatcaaaccc ttttgtgaag atcttgacca atggctaagt 
     5041 gaagatgaca atcatgttgc agcaattcac tgtaaagctg gaaagggacg aactggtgta 
     5101 atgatatgtg catatttatt acatcggggc aaatttttaa aggcacaaga ggccctagat 
     5161 ttctatgggg aagtaaggac cagagacaaa aagggagtaa ctattcccag tcagaggcgc 
     5221 tatgtgtatt attatagcta cctgttaaag aatcatctgg attatagacc agtggcactg 
     5281 ttgtttcaca agatgatgtt tgaaactatt ccaatgttca gtggcggaac ttgcaatcct 
     5341 cagtttgtgg tctgccagct aaaggtcaaa atttactcgt ccaattcagg acccacacga 
     5401 cgggaagaca agttcatgta ctttgagttc cctcagccgt tacctgtgtg tggtggtatc 
     5461 aaagtagagt tcttccacaa acagaacaag atgctaaaaa aggacaaaat gtttcacttt 
     5521 tgggtaaata cattcttcat accaggacca gaggaaacct cagaaaaagt agaaaatgga 
     5581 agtctatgtg atcaagaaat cgatagcatt tgcagtatag agcgtgcaga taatgacaag 
     5641 gaatatctag tacttacttt aacaaaaaat gatcttgaca aagcaaataa agacaaagcc 
     5701 aaccgatact tttctccaaa ttttaaggtg aagctgtact tcacaaaaac agtagaggag 
     5761 ccgtcaaatc cagaggctag cagttcaact tctgtaacac cagatgttag tgacaatgaa 
     5821 cctgatcatt atagatattc tgacaccact gactctgatc cagagaatga accttttgat 
     5881 gaagatcagc atacacaaat tacaaaagtc tgagaattcg atatcaagct tatcgataat 
     5941 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 
     6001 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 
     6061 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 
     6121 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 
     6181 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 
     6241 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 
     6301 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc 
     6361 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 
     6421 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 
     6481 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga 
     6541 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct 
     6601 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag 
     6661 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 
     6721 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg 
     6781 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc 
     6841 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta 
     6901 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg 
     6961 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac 
     7021 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc 
     7081 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 
     7141 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 
     7201 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc 
     7261 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac 
     7321 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg 
     7381 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga 
     7441 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga 
     7501 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc 
     7561 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 
     7621 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 
     7681 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 
     7741 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 
     7801 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 
     7861 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 
     7921 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt 
     7981 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 
     8041 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 
     8101 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 
     8161 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 
     8221 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt 
     8281 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct 
     8341 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca 
     8401 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg 
     8461 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg 
     8521 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg 
     8581 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt 
     8641 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga 
     8701 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact 
     8761 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg 
     8821 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 
     8881 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt 
     8941 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 
     9001 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt 
     9061 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 
     9121 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 
     9181 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 
     9241 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 
     9301 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 
     9361 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 
     9421 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 
     9481 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     9541 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     9601 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     9661 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 
     9721 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     9781 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     9841 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     9901 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 
     9961 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
    10021 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 
    10081 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
    10141 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 
    10201 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 
    10261 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 
    10321 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 
    10381 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 
    10441 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 
    10501 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 
    10561 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 
    10621 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 
    10681 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 
    10741 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 
    10801 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 
    10861 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 
    10921 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 
    10981 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 
    11041 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 
    11101 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 
    11161 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 
    11221 catttccccg aaaagtgcca cctgac 
//
