LOCUS       Exported                8509 bp ds-DNA     circular SYN 21-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Jun 21, 2018 from SnapGene 4.1.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    7910..7912
                     /label=Kozak
     misc_feature    7913..8509
                     /label=Bim-EL ORF
ORIGIN
        1 atccagcaca gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat
       61 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
      121 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
      181 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
      241 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
      301 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
      361 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
      421 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
      481 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
      541 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
      601 gggccgcctc cccgcctgga attctgcaga tatacggact agtgatctaa ttctaccggg
      661 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
      721 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
      781 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
      841 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
      901 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
      961 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     1021 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     1081 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     1141 catctccggg cctttcgacc tgcagccaat atgggatcgg ccattgaaca agatggattg
     1201 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     1261 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     1321 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     1381 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     1441 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     1501 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     1561 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     1621 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     1681 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     1741 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     1801 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     1861 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     1921 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat
     1981 ccgtcgaggg cccggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     2041 actttttaaa agaaaagggg ggactggaag ggctagctca ctcccaacga agacaagatc
     2101 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     2161 gctgcctagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     2221 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     2281 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     2341 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     2401 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2461 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     2521 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     2581 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     2641 aggcctaggc ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg
     2701 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     2761 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     2821 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc
     2881 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     2941 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3001 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3061 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     3121 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3181 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     3241 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     3301 aaaatattaa cgtttacaat ttcctcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3361 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3421 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3481 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3541 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3601 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3661 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3721 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3781 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3841 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3901 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3961 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4021 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4081 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4141 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4201 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4261 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4321 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4381 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4441 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4501 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4561 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4621 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4681 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4741 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4801 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4861 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4921 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4981 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5041 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5101 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5161 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5221 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     5281 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     5341 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     5401 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     5461 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     5521 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     5581 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     5641 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     5701 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     5761 gatacaataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5821 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5881 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5941 aaaatctcta gcagtggcgc ccgaacaggg acctgaaagc gaaagggaaa ccagagctct
     6001 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     6061 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     6121 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     6181 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     6241 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     6301 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     6361 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     6421 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     6481 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     6541 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     6601 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     6661 ggcgcagcct caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     6721 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     6781 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     6841 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     6901 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     6961 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     7021 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     7081 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     7141 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     7201 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     7261 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     7321 ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca
     7381 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     7441 attacaaaaa ttcaaaattt tatcgatcac gagactagcc tcgagagttg gctttactcc
     7501 ctatcagtga tagagaacgt atgaagagtt tactccctat cagtgataga gaacgtatgc
     7561 agactttact ccctatcagt gatagagaac gtataaggag tttactccct atcagtgata
     7621 gagaacgtat gaccagttta ctccctatca gtgatagaga acgtatctac agtttactcc
     7681 ctatcagtga tagagaacgt atatccagtt tactccctat cagtgataga gaacgtataa
     7741 gctttaggcg tgtacggtgg gcgcctataa aagcagagct cgtttagtga accgtcagat
     7801 cgcctggagc aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc
     7861 ctcgtaaaac cggtcgtcga ctagtccagt gtggtggaat tctgcagata ccatggcaaa
     7921 gcaaccttct gatgtaagtt ctgagtgtga ccgagaaggt agacaattgc agcctgcgga
     7981 gaggcctccc cagctcagac ctggggcccc tacctcccta cagacagagc cacaaggtaa
     8041 tcctgaaggc aatcacggag gtgaagggga cagctgcccc cacggcagcc ctcagggccc
     8101 gctggcccca cctgccagcc ctggcccttt tgctaccaga tccccgcttt tcatctttat
     8161 gagaagatcc tccctgctgt ctcgatcctc cagtgggtat ttctcttttg acacagacag
     8221 gagcccagca cccatgagtt gtgacaaatc aacacaaacc ccaagtcctc cttgccaggc
     8281 cttcaaccac tatctcagtg caatggcttc catgaggcag gctgaacctg cagatatgcg
     8341 cccagagata tggatcgccc aagagttgcg gcgtattgga gacgagttta acgcttacta
     8401 tgcaaggagg gtatttttga ataattacca agcagccgaa gaccacccac gaatggttat
     8461 cttacgactg ttacgttaca ttgtccgcct ggtgtggaga atgcattga
//
