LOCUS       Exported               10358 bp ds-DNA     circular SYN 09-FEB-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Sho Yoshimatsu
  TITLE     Direct Submission
  JOURNAL   Exported Feb 13, 2018 from SnapGene 4.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     439..458
                     /label=f1ori-3
     primer_bind     complement(504..514)
                     /label=NheI-hG-5
     primer_bind     552..571
                     /label=pCR-BEnd-3
     primer_bind     596..616
                     /label=AgeI-M13fwd-5
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     600..616
                     /label=M13 fwd
     primer_bind     600..616
                     /label=pDEST-M13fwd
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     623..641
                     /label=T7-5
     misc_feature    complement(656..965)
                     /gene="PB5'TR"
                     /label=PB5'TR
     primer_bind     656..680
                     /label=XhoI-PBR-R
     primer_bind     complement(785..802)
                     /label=PBR-F
     primer_bind     complement(869..891)
                     /label=PBLm-F2
     primer_bind     943..986
                     /label=bGHpA-5'PB-R
     primer_bind     968..982
                     /label=bGHpA-cjPLP1-CDS2-3
     polyA_signal    complement(969..1176)
                     /label=bGH poly(A) signal
     primer_bind     969..988
                     /label=pUCDESTAvrII-bGHpA-3
     primer_bind     969..988
                     /label=bGHpA-BlnI-3
     primer_bind     969..986
                     /label=bGHpA-R
     primer_bind     1022..1043
                     /label=bGHpA-3
     primer_bind     complement(1157..1196)
                     /label=RfA-bGHpA-F
     primer_bind     complement(1161..1176)
                     /label=EGFP-TAA-bGHpA-5
     protein_bind    1198..1322
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    complement(1199..1219)
                     /label=att2 core
                     /note="att2
                     CARCTTT!CTTGTAC AAAGYKG
                     GTYGAAA GAACATG!TTTCRMC"
     primer_bind     complement(1205..1233)
                     /label=attR1-F
     primer_bind     complement(1263..1288)
                     /label=attR1-R
     primer_bind     complement(1332..1351)
                     /label=pE4-attR4-5
     CDS             complement(1363..1668)
                     /codon_start=1
                     /label=ccdB
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1615..1638
                     /label=ccdB-3
     primer_bind     complement(1649..1668)
                     /label=ccdB start-5
     primer_bind     complement(1863..1882)
                     /label=CmdB-5
     CDS             complement(2010..2669)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2144..2163)
                     /label=CmR-5
     primer_bind     complement(2459..2475)
                     /label=CmR-EcoRI-5
     primer_bind     2634..2658
                     /label=CmR-R
     promoter        complement(2723..2753)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(2728..2747)
                     /label=Lac pro-5
     protein_bind    complement(2778..2902)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     2812..2837
                     /label=attR1-R
     primer_bind     2867..2895
                     /label=attR1-F
     misc_feature    2881..2901
                     /label=att1 core
                     /note="att1
                     CWRCTTT!TTTGTAC AAASTTG
                     GWYGAAA AAACATG!TTTSAAC"
     promoter        complement(2912..3226)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     promoter        complement(2913..2972)
                     /label=minimal CMV promoter
     primer_bind     complement(2913..2932)
                     /label=mCMV-F
     protein_bind    complement(2986..3004)
                     /label=tet operator(6)
     protein_bind    complement(3022..3040)
                     /label=tet operator(5)
     protein_bind    complement(3057..3075)
                     /label=tet operator(4)
     protein_bind    complement(3093..3111)
                     /label=tet operator(3)
     protein_bind    complement(3129..3147)
                     /label=tet operator(2)
     protein_bind    complement(3164..3182)
                     /label=tet operator(1)
     protein_bind    complement(3200..3218)
                     /label=tet operator
     primer_bind     3235..3254
                     /label=TRE-EF-I
     primer_bind     complement(3235..3254)
                     /label=TRE-EF-II
     promoter        3263..4425
                     /label=EF1a promoter
     primer_bind     3263..3278
                     /label=KpnI-EF-5
     primer_bind     3263..3278
                     /label=EF-F
     primer_bind     complement(3352..3362)
                     /label=cjPLP1-aG2+7k-5
     intron          3494..4416
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3661..3670
                     /label=HPRTe2-s2
     primer_bind     complement(3755..3769)
                     /label=cjHPRTgg-R2
     primer_bind     3840..3854
                     /label=ApaI-EF-F
     primer_bind     3874..3884
                     /label=SOX2-gRNAmut-F
     primer_bind     complement(3949..3958)
                     /label=BRCA2-R1
     primer_bind     4014..4025
                     /label=pSp-AgeI-5
     primer_bind     4335..4354
                     /label=EFi-5
     primer_bind     4415..4458
                     /label=ef-iRFP-F
     primer_bind     complement(4415..4437)
                     /label=EF-R
     primer_bind     4438..4456
                     /label=Bam-k-iRFP-F
     primer_bind     4438..4454
                     /label=gi-iRFP-F
     regulatory      4438..4447
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     4438..4447
                     /label=T7-pDONR(CAG)-F
     CDS             4444..5391
                     /codon_start=1
                     /label=iRFP713
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     primer_bind     4444..4459
                     /label=P2A-iRFP-5
     primer_bind     complement(4789..4801)
                     /label=cjDPPA5-F1
     primer_bind     complement(4852..4875)
                     /label=iRFP-Delta-S-R
     primer_bind     4857..4881
                     /label=iRFP-Delta-S-F
     primer_bind     complement(5023..5034)
                     /label=SOX2-PvuII-F
     primer_bind     5048..5063
                     /label=mCherry-Delta-S2-R
     primer_bind     5369..5402
                     /label=BsrGI-T2A-5
     primer_bind     complement(5371..5411)
                     /label=T2A-iRFP-R
     primer_bind     complement(5371..5391)
                     /label=SalI-iRFP-R
     primer_bind     complement(5373..5391)
                     /label=iRFP-5'TR-3
     primer_bind     5390..5468
                     /label=FP-T2A-Puro-F
     CDS             5392..5445
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     5392..5411
                     /label=T2A-F
     primer_bind     complement(5393..5404)
                     /label=BRCA2-F2
     primer_bind     complement(5427..5441)
                     /label=T2A-R
     primer_bind     5430..5471
                     /label=T2A-MS-Puro-F
     CDS             5446..5449
                     /codon_start=1
                     /label=rtTA2M2
                     /translation="M"
     CDS             5452..6048
                     /codon_start=1
                     /label=PuroR
                     /label=Pac
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5452..5471
                     /label=CACC-Puro-5
     primer_bind     5452..5471
                     /label=T2A-Puro-F
     primer_bind     5452..5466
                     /label=NotI-k-Puro-5
     primer_bind     5479..5498
                     /label=Puro-AatII-5
     primer_bind     5479..5488
                     /label=PV-gRNAmut-F
     primer_bind     5640..5656
                     /label=Puro-5
     primer_bind     complement(5728..5737)
                     /label=pDCAG-RAD51C-F
     primer_bind     5755..5769
                     /label=Neo End(pROX)-3
     primer_bind     5765..5783
                     /label=Puro111-F
     primer_bind     complement(5773..5791)
                     /label=cjPLP1 mRNA-5(2)
     primer_bind     complement(5932..5952)
                     /label=Puro166R-R
     primer_bind     complement(5933..5954)
                     /label=Puro-H166Rmut-3
     primer_bind     5935..5952
                     /label=Puro166R-F
     primer_bind     5942..5963
                     /label=Puro-H166Rmut-5
     primer_bind     complement(6029..6048)
                     /label=Puro-delStop-XhoI-BamHI-3
     primer_bind     complement(6029..6048)
                     /label=Puro-delStop-SnaBI-BamHI-3
     primer_bind     complement(6034..6086)
                     /label=Puro-P2A-R
     primer_bind     complement(6034..6048)
                     /label=Puro-pPB-R
     primer_bind     complement(6034..6048)
                     /label=Puro-3
     primer_bind     complement(6034..6048)
                     /label=pCXLE-Puro-R
     CDS             6049..6105
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     6049..6067
                     /label=FokI-P2A-5
     primer_bind     6049..6066
                     /label=rTAM2-P2A-F
     primer_bind     complement(6066..6091)
                     /label=P2A-deltaEsp3I-3
     primer_bind     6072..6125
                     /label=P2A-rtTA-F
     primer_bind     6077..6102
                     /label=P2A-deltaEsp3I-5
     primer_bind     complement(6082..6101)
                     /label=P2A-3
     CDS             6106..6852
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             6106..6846
                     /codon_start=1
                     /label=rtTA2M2
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLP"
     primer_bind     complement(6257..6275)
                     /label=ApaI-rtTA-R
     primer_bind     6647..6664
                     /label=rtTA-F
     primer_bind     complement(6868..6892)
                     /label=CSIV-Xba-R
     primer_bind     6897..6927
                     /label=BW-F
     primer_bind     6899..6916
                     /label=WPRE-F
     primer_bind     6899..6914
                     /label=BA-WPRE-F
     misc_feature    6902..7490
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     6902..6925
                     /label=BamHI-W-F
     primer_bind     7138..7155
                     /label=W-F
     primer_bind     complement(7138..7155)
                     /label=W-R
     primer_bind     complement(7368..7378)
                     /label=Bam-k-iRFP-F
     CDS             complement(7373..7384)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7444..7460
                     /label=Hyg-XbaI-3
     primer_bind     complement(7485..7494)
                     /label=WPRE-pPBCla-R
     primer_bind     complement(7485..7494)
                     /label=BW-R
     primer_bind     complement(7485..7494)
                     /label=SacI-W-R
     primer_bind     complement(7731..7752)
                     /label=BglII-SV40LT-R
     primer_bind     complement(7765..7784)
                     /label=Octet-MfeI-5
     polyA_signal    7774..7895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7894..7917)
                     /label=SV40pA-BamHI-3
     misc_feature    7916..8150
                     /gene="PB3'TR"
                     /label=PB3'TR
     primer_bind     complement(8121..8150)
                     /label=PBF-R
     primer_bind     complement(8124..8150)
                     /label=NcoI-NotI-PBLm-5
     primer_bind     complement(8129..8150)
                     /label=Sfo-cassette-5
     promoter        complement(8170..8188)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8170..8188)
                     /label=T3-5
     primer_bind     complement(8209..8225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8209..8225)
                     /label=M13 rev
     primer_bind     complement(8209..8225)
                     /label=pDEST-M13rev
     primer_bind     complement(8230..8249)
                     /label=Lac ope-5
     protein_bind    8233..8249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8257..8287)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(8262..8281)
                     /label=Lac pro-5
     protein_bind    8302..8323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8302..8323)
                     /label=CAP-5
     primer_bind     complement(8410..8429)
                     /label=pCRstart-5
     primer_bind     complement(8556..8575)
                     /label=pPB-PciI-R
     primer_bind     8556..8573
                     /label=pSp-XbaI-3
     rep_origin      complement(8611..9199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8611..8630)
                     /label=Ori-end-5
     primer_bind     8862..8878
                     /label=Ori-ApaLI-3
     primer_bind     complement(8869..8888)
                     /label=Ori-ApaLI-5
     primer_bind     complement(8922..8943)
                     /label=Ori-5
     primer_bind     9180..9199
                     /label=Ori-start-3
     primer_bind     complement(9247..9266)
                     /label=AO-5
     CDS             complement(9370..10230)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9613..9632
                     /label=Amp-3
     primer_bind     complement(9663..9682)
                     /label=Amp-5
     primer_bind     complement(9844..9863)
                     /label=Amp422-5
     primer_bind     9904..9931
                     /label=Amp-ScaIfus-R
     primer_bind     9924..9943
                     /label=Amp-ScaI-3
     promoter        complement(10231..10335)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10231..10250
                     /label=AmpP-end-3
     primer_bind     complement(10281..10300)
                     /label=AmpP-5
     primer_bind     complement(10285..10335)
                     /label=pAmp2-Link-5
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatctc cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc
     1021 ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat
     1081 tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg
     1141 ggcaaacaac agatggctgg caactagaag gcacagatcg tcgagaccgg tagcatcacc
     1201 actttgtaca agaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
     1261 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
     1321 tggtcgacct gcagactggc tgtgtataag ggagcctgac atttatattc cccagaacat
     1381 caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc
     1441 gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc
     1501 gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc
     1561 cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa
     1621 cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt caccagtccc
     1681 tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt
     1741 cctgattttc cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg
     1801 tgcttaccag accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct
     1861 gtcaactgtc actgtaatac gctgcttcat agcacacctc tttttgacat acttcgggta
     1921 tacatatcag tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat
     1981 ctggctttta gtaagccgga tccacgcgtt tacgccccgc cctgccactc atcgcagtac
     2041 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc
     2101 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     2161 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     2221 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     2281 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     2341 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     2401 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
     2461 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     2521 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     2581 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     2641 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgcc
     2701 ggatcctaac tcaaaatcca cacattatac gagccggaag cataaagtgt aaagcctggg
     2761 gtgcctaatg cggccgccat agtgactgga tatgttgtgt tttacagtat tatgtagtct
     2821 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt
     2881 cagctttttt gtacaaactt gtgatgctag cggcgatctg acggttcact aaacgagctc
     2941 tgcttatata ggcctcccac cgtacacgcc tacctcgaca tacgttctct atcactgata
     3001 gggagtaaac tcgacatacg ttctctatca ctgataggga taaactcgac atacgttctc
     3061 tatcactgat agggagtaaa ctcgacatac gttctctatc actgataggg agtaaactcg
     3121 acatacgttc tctatcactg atagggagta aactcgacat cgttctctat cactgatagg
     3181 gagtaaactc gacatacgtt ctctatcact gatagggagt aaactcgatt gaagacgaaa
     3241 gggccgaatt caagcttcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     3301 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
     3361 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     3421 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     3481 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     3541 tggcccttgc gtgccttgaa ttacttccac ctggctccag tacgtgattc ttgatcccga
     3601 gctggagcca ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag
     3661 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     3721 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     3781 ttctggcaag atagtcttgt aaatgcgggc caggatctgc acactggtat ttcggttttt
     3841 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     3901 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     3961 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     4021 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctccagg gggctcaaaa
     4081 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaggggcc
     4141 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     4201 ctcgattagt tctggagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     4261 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     4321 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     4381 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaacacg ctaccgggcc
     4441 accatggccg aagggagcgt ggcaagacag cccgatctgc tgacatgcga cgacgaacca
     4501 atccacattc caggagctat ccagcctcat ggactgctgc tggctctggc cgctgacatg
     4561 actatcgtgg caggctctga taacctgcca gagctgaccg ggctggctat cggagcactg
     4621 attggccggt ccgcagccga cgtgttcgat tctgagactc acaatagact gaccatcgcc
     4681 ctggctgaac ctggggctgc agtgggagcc ccaattaccg tcggcttcac aatgcgaaag
     4741 gacgctggct ttatcgggtc ctggcaccgc catgatcagc tgatttttct ggagctggaa
     4801 ccacctcaga gagacgtggc agagccacag gctttctttc ggagaacaaa cagcgccatc
     4861 aggcgcctgc aggccgctga gactctggaa tccgcttgcg cagccgctgc acaggaagtg
     4921 aggaagatca ccgggttcga tcgcgtcatg atctacagat tcgcctcaga ctttagcgga
     4981 gaagtgatcg cagaagatcg ctgtgccgag gtcgaaagta aactgggcct gcattaccca
     5041 gcctcaaccg tgcctgcaca ggcacgacgg ctgtatacaa tcaaccccgt gcggatcatt
     5101 cccgacatta attacagacc agtgcccgtc acacctgacc tgaacccagt gactgggagg
     5161 cccatcgatc tgagcttcgc cattctgcgc agcgtgtccc ctgtccacct ggagtttatg
     5221 aggaatatcg gaatgcatgg caccatgtct atcagtattc tgagaggcga aaggctgtgg
     5281 gggctgatcg tgtgccacca tcggacacct tactatgtgg acctggacgg caggcaggct
     5341 tgtgaactgg tggctcaggt gctggcttgg cagattggcg tgatggaaga agagggcaga
     5401 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgtc tatgaccgag
     5461 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     5521 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     5581 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     5641 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     5701 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     5761 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     5821 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     5881 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     5941 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6001 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgc cacgaacttc
     6061 tctctgttaa agcaagcagg agacgtggaa gaaaaccccg gtcctatgtc tagactggac
     6121 aagagcaaag tcataaacgg cgctctggaa ttactcaatg gagtcggtat cgaaggcctg
     6181 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
     6241 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
     6301 cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
     6361 ttccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
     6421 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
     6481 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
     6541 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
     6601 atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
     6661 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
     6721 ggcgggccgg ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
     6781 gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg
     6841 ctccccgggt aactaagtaa ggatcctcta gagttaacat cgagggatca agcttatcga
     6901 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6961 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7021 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7081 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7141 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7201 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7261 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7321 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7381 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7441 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     7501 tcgagacctg gaaaaacatg gagcaatcac aagtagcaac acagcagcta ccaatgctgc
     7561 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     7621 acctttaaga ccaatgactt acaaggcagc tgtagatcta gataactgat cataatcagc
     7681 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     7741 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     7801 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7861 agttgtggtt tgtccaaact catcaatgta tcttaacgct cgagtgcatg gatcctttgt
     7921 tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata aacataaata
     7981 aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac ttaatatcta
     8041 ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt caattttacg
     8101 catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa agagctccag
     8161 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     8221 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8281 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8341 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8401 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8461 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8521 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8581 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8641 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9061 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9121 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9181 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9241 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9301 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9361 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9421 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9481 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9541 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9601 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9661 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9721 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9781 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9841 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9901 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9961 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10021 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10081 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10141 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10201 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10261 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10321 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
