LOCUS       Exported                9558 bp ds-DNA     circular SYN 29-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Sho Yoshimatsu
  TITLE     Direct Submission
  JOURNAL   Exported Aug 5, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     439..458
                     /label=f1ori-3
     primer_bind     complement(504..514)
                     /label=NheI-hG-5
     primer_bind     552..571
                     /label=pCR-BEnd-3
     primer_bind     596..616
                     /label=AgeI-M13fwd-5
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     600..616
                     /label=pDEST-M13fwd
     primer_bind     600..616
                     /label=M13 fwd
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     623..641
                     /label=T7-5
     misc_feature    complement(656..965)
                     /gene="PB5'TR"
                     /label=PB5'TR
     primer_bind     656..680
                     /label=XhoI-PBR-R
     primer_bind     complement(785..802)
                     /label=PBR-F
     primer_bind     complement(869..891)
                     /label=PBLm-F2
     primer_bind     943..986
                     /label=bGHpA-5'PB-R
     primer_bind     968..982
                     /label=bGHpA-cjPLP1-CDS2-3
     polyA_signal    complement(969..1176)
                     /label=bGH poly(A) signal
     primer_bind     969..988
                     /label=bGHpA-BlnI-3
     primer_bind     969..988
                     /label=pUCDESTAvrII-bGHpA-3
     primer_bind     969..986
                     /label=bGHpA-R
     primer_bind     1022..1043
                     /label=bGHpA-3
     primer_bind     complement(1157..1196)
                     /label=RfA-bGHpA-F
     primer_bind     complement(1161..1176)
                     /label=EGFP-TAA-bGHpA-5
     protein_bind    1198..1222
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(1199..1205)
                     /label=att2 core
                     /note="att2
                     CARCTTT!CTTGTAC AAAGYKG
                     GTYGAAA GAACATG!TTTCRMC"
     misc_feature    1206..1222
                     /label=attL2
     misc_feature    complement(1206..1219)
                     /label=att2 core
                     /note="att2
                     CARCTTT!CTTGTAC AAAGYKG
                     GTYGAAA GAACATG!TTTCRMC"
     misc_feature    1238
                     /label=ORF(1)
     CDS             complement(1239..2057)
                     /codon_start=1
                     /label=NGN2
                     /translation="MFVKSETLELKEEEDVLVLLGSASPALAALTPLSSSADEEEEEEP
                     GASGGARRQRGAEAGQGARGGVAAGAEGCRPARLLGLVHDCKRRPSRARAVSRGAKTAE
                     TVQRIKKTRRLKANNRERNRMHNLNAALDALREVLPTFPEDAKLTKIETLRFAHNYIWA
                     LTETLRLADHCGGGGGGLPGALFSEAVLLSPGGASAALSSSGDSPSPASTWSCTNSPAP
                     SSSVSSNSTSPYSCTLSPASPAGSDMDYWQPPPPDKHRYAPHLPIARDCI"
     primer_bind     1242..1263
                     /label=NGN2-T2A-R
     primer_bind     1328..1338
                     /label=pCXLE-Puro-R
     primer_bind     1328..1338
                     /label=Puro-P2A-R
     primer_bind     1328..1338
                     /label=Puro-3
     primer_bind     1328..1338
                     /label=Puro-pPB-R
     misc_feature    1470
                     /label=a->c(Gly->Gly)
     misc_feature    1911
                     /label=c->t(Gly->Gly)
     primer_bind     complement(2040..2093)
                     /label=pENTR-NGN2-F
     protein_bind    complement(2078..2102)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2079..2087
                     /label=attL1
     misc_feature    2081..2087
                     /label=att1 core
                     /note="att1
                     CWRCTTT!TTTGTAC AAASTTG
                     GWYGAAA AAACATG!TTTSAAC"
     misc_feature    2088..2101
                     /label=att1 core
                     /note="att1
                     CWRCTTT!TTTGTAC AAASTTG
                     GWYGAAA AAACATG!TTTSAAC"
     promoter        complement(2112..2426)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     promoter        complement(2113..2172)
                     /label=minimal CMV promoter
     primer_bind     complement(2113..2132)
                     /label=mCMV-F
     protein_bind    complement(2186..2204)
                     /label=tet operator(6)
     protein_bind    complement(2222..2240)
                     /label=tet operator(5)
     protein_bind    complement(2257..2275)
                     /label=tet operator(4)
     protein_bind    complement(2293..2311)
                     /label=tet operator(3)
     protein_bind    complement(2329..2347)
                     /label=tet operator(2)
     protein_bind    complement(2364..2382)
                     /label=tet operator(1)
     protein_bind    complement(2400..2418)
                     /label=tet operator
     primer_bind     complement(2435..2454)
                     /label=TRE-EF-II
     primer_bind     2435..2454
                     /label=TRE-EF-I
     promoter        2463..3625
                     /label=EF1a promoter
     primer_bind     2463..2478
                     /label=EF-F
     primer_bind     2463..2478
                     /label=KpnI-EF-5
     primer_bind     complement(2552..2562)
                     /label=cjPLP1-aG2+7k-5
     intron          2694..3616
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2861..2870
                     /label=HPRTe2-s2
     primer_bind     complement(2955..2969)
                     /label=cjHPRTgg-R2
     primer_bind     3040..3054
                     /label=ApaI-EF-F
     primer_bind     3074..3084
                     /label=SOX2-gRNAmut-F
     primer_bind     complement(3149..3158)
                     /label=BRCA2-R1
     primer_bind     3214..3225
                     /label=pSp-AgeI-5
     primer_bind     3535..3554
                     /label=EFi-5
     primer_bind     3615..3658
                     /label=ef-iRFP-F
     primer_bind     complement(3615..3637)
                     /label=EF-R
     primer_bind     3626..3654
                     /label=pPB-NotI-HpaI-iRFP-F
     primer_bind     3638..3656
                     /label=Bam-k-iRFP-F
     primer_bind     3638..3654
                     /label=gi-iRFP-F
     regulatory      3638..3647
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     3638..3647
                     /label=T7-pDONR(CAG)-F
     CDS             3644..4591
                     /codon_start=1
                     /label=iRFP713
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     primer_bind     3644..3659
                     /label=P2A-iRFP-5
     primer_bind     complement(3989..4001)
                     /label=cjDPPA5-F1
     primer_bind     complement(4052..4075)
                     /label=iRFP-Delta-S-R
     primer_bind     4057..4081
                     /label=iRFP-Delta-S-F
     primer_bind     complement(4223..4234)
                     /label=SOX2-PvuII-F
     primer_bind     4248..4263
                     /label=mCherry-Delta-S2-R
     primer_bind     4569..4602
                     /label=BsrGI-T2A-5
     primer_bind     complement(4571..4611)
                     /label=T2A-iRFP-R
     primer_bind     complement(4571..4591)
                     /label=SalI-iRFP-R
     primer_bind     complement(4573..4591)
                     /label=iRFP-5'TR-3
     primer_bind     4590..4668
                     /label=FP-T2A-Puro-F
     CDS             4592..4645
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     4592..4611
                     /label=T2A-F
     primer_bind     complement(4593..4604)
                     /label=BRCA2-F2
     primer_bind     complement(4627..4645)
                     /label=T2A-R2
     primer_bind     complement(4627..4641)
                     /label=T2A-R
     primer_bind     4630..4671
                     /label=T2A-MS-Puro-F
     CDS             4646..4649
                     /codon_start=1
                     /label=rtTA2M2
                     /translation="M"
     CDS             4652..5248
                     /codon_start=1
                     /label=PuroR
                     /label=Pac
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4652..4671
                     /label=T2A-Puro-F
     primer_bind     4652..4671
                     /label=CACC-Puro-5
     primer_bind     4652..4666
                     /label=NotI-k-Puro-5
     primer_bind     4679..4698
                     /label=Puro-AatII-5
     primer_bind     4679..4688
                     /label=PV-gRNAmut-F
     primer_bind     4840..4856
                     /label=Puro-5
     primer_bind     complement(4928..4937)
                     /label=pDCAG-RAD51C-F
     primer_bind     4955..4969
                     /label=Neo End(pROX)-3
     primer_bind     4965..4983
                     /label=Puro111-F
     primer_bind     complement(4973..4991)
                     /label=cjPLP1 mRNA-5(2)
     primer_bind     complement(5132..5152)
                     /label=Puro166R-R
     primer_bind     complement(5133..5154)
                     /label=Puro-H166Rmut-3
     primer_bind     5135..5152
                     /label=Puro166R-F
     primer_bind     5142..5163
                     /label=Puro-H166Rmut-5
     primer_bind     complement(5229..5248)
                     /label=Puro-delStop-SnaBI-BamHI-3
     primer_bind     complement(5229..5248)
                     /label=Puro-delStop-XhoI-BamHI-3
     primer_bind     complement(5234..5286)
                     /label=Puro-P2A-R
     primer_bind     complement(5234..5248)
                     /label=pCXLE-Puro-R
     primer_bind     complement(5234..5248)
                     /label=Puro-3
     primer_bind     complement(5234..5248)
                     /label=Puro-pPB-R
     CDS             5249..5305
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     5249..5267
                     /label=FokI-P2A-5
     primer_bind     5249..5266
                     /label=rTAM2-P2A-F
     primer_bind     complement(5266..5291)
                     /label=P2A-deltaEsp3I-3
     primer_bind     5272..5325
                     /label=P2A-rtTA-F
     primer_bind     5277..5302
                     /label=P2A-deltaEsp3I-5
     primer_bind     complement(5282..5301)
                     /label=P2A-3
     CDS             5306..6052
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             5306..6046
                     /codon_start=1
                     /label=rtTA2M2
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLP"
     primer_bind     complement(5457..5475)
                     /label=ApaI-rtTA-R
     primer_bind     5847..5864
                     /label=rtTA-F
     primer_bind     complement(6068..6092)
                     /label=CSIV-Xba-R
     primer_bind     6097..6127
                     /label=BW-F
     primer_bind     6099..6116
                     /label=WPRE-F
     primer_bind     6099..6114
                     /label=BA-WPRE-F
     misc_feature    6102..6690
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     6102..6125
                     /label=BamHI-W-F
     primer_bind     complement(6338..6355)
                     /label=W-R
     primer_bind     6338..6355
                     /label=W-F
     primer_bind     complement(6568..6578)
                     /label=Bam-k-iRFP-F
     CDS             complement(6573..6584)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6644..6660
                     /label=Hyg-XbaI-3
     primer_bind     complement(6685..6694)
                     /label=SacI-W-R
     primer_bind     complement(6685..6694)
                     /label=BW-R
     primer_bind     complement(6685..6694)
                     /label=WPRE-pPBCla-R
     primer_bind     complement(6931..6952)
                     /label=BglII-SV40LT-R
     primer_bind     complement(6965..6984)
                     /label=Octet-MfeI-5
     polyA_signal    6974..7095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7094..7117)
                     /label=SV40pA-BamHI-3
     misc_feature    7116..7350
                     /gene="PB3'TR"
                     /label=PB3'TR
     primer_bind     complement(7321..7350)
                     /label=PBF-R
     primer_bind     complement(7324..7350)
                     /label=NcoI-NotI-PBLm-5
     primer_bind     complement(7329..7350)
                     /label=Sfo-cassette-5
     promoter        complement(7370..7388)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7370..7388)
                     /label=T3-5
     primer_bind     complement(7409..7425)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7409..7425)
                     /label=pDEST-M13rev
     primer_bind     complement(7409..7425)
                     /label=M13 rev
     primer_bind     complement(7430..7449)
                     /label=Lac ope-5
     protein_bind    7433..7449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7457..7487)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(7462..7481)
                     /label=Lac pro-5
     protein_bind    7502..7523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7502..7523)
                     /label=CAP-5
     primer_bind     complement(7610..7629)
                     /label=pCRstart-5
     primer_bind     complement(7756..7775)
                     /label=pPB-PciI-R
     primer_bind     7756..7773
                     /label=pSp-XbaI-3
     rep_origin      complement(7811..8399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7811..7830)
                     /label=Ori-end-5
     primer_bind     8062..8078
                     /label=Ori-ApaLI-3
     primer_bind     complement(8069..8088)
                     /label=Ori-ApaLI-5
     primer_bind     complement(8122..8143)
                     /label=Ori-5
     primer_bind     8380..8399
                     /label=Ori-start-3
     primer_bind     complement(8447..8466)
                     /label=AO-5
     CDS             complement(8570..9430)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8813..8832
                     /label=Amp-3
     primer_bind     complement(8863..8882)
                     /label=Amp-5
     primer_bind     complement(9044..9063)
                     /label=Amp422-5
     primer_bind     9104..9131
                     /label=Amp-ScaIfus-R
     primer_bind     9124..9143
                     /label=Amp-ScaI-3
     promoter        complement(9431..9535)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9431..9450
                     /label=AmpP-end-3
     primer_bind     complement(9481..9500)
                     /label=AmpP-5
     primer_bind     complement(9485..9535)
                     /label=pAmp2-Link-5
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatctc cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc
     1021 ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat
     1081 tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg
     1141 ggcaaacaac agatggctgg caactagaag gcacagatcg tcgagaccgg tagcatcacc
     1201 actttgtaca agaaagctgg gtcggcgcgc ccacccttct agatacaatc cctggctatg
     1261 gggaggtgag gtgcatagcg gtgcttgtcg ggaggtgggg gctgccaata gtccatgtct
     1321 gacccggccg ggctggcggg cgataaagtg cagctgtagg gggaggtgga attggaggac
     1381 acggaggagg acggcgcggg gctgttggtg caactccacg tggaggcggg cgaggggctg
     1441 tctccgctgc tgctcagggc ggcgctggcg cctcccgggc tcagcaacac tgcctcggag
     1501 aagagcgccc ccggcaggcc cccgccgccg cccccgcagt gatccgccag gcgcagggtc
     1561 tcggtgagtg cccagatgta gttgtgggcg aagcgcaggg tctcgatctt ggtgagcttg
     1621 gcgtcctcgg ggaacgtggg gagcacctcg cgcagcgcgt ccagtgccgc gttgaggttg
     1681 tgcatgcggt ttcgctcgcg gttgttggcc ttcagtctac gggtcttctt gatgcgctgc
     1741 accgtctcgg ccgtcttggc gcctcgggag acggcccgcg cccgggaagg gcgccgtttg
     1801 caatcgtgta ccagacccag cagccgtgcg ggccggcagc cctccgcacc cgcagccacg
     1861 ccgccccgcg ccccctgccc ggcctcagcc ccgcgctgcc gacgcgcccc acctgacgcg
     1921 cccggctcct cctcctcttc ttcgtcggcg ctggatgaca gcggggtcag ggccgccaag
     1981 gcgggggagg ccgatccgag cagcactaac acgtcctctt cctccttcaa ctccaaggtc
     2041 tcggatttga cgaacatggt gaagggggcg gccgcggagc ctgctttttt gtacaaactt
     2101 gtgatgctag cggcgatctg acggttcact aaacgagctc tgcttatata ggcctcccac
     2161 cgtacacgcc tacctcgaca tacgttctct atcactgata gggagtaaac tcgacatacg
     2221 ttctctatca ctgataggga taaactcgac atacgttctc tatcactgat agggagtaaa
     2281 ctcgacatac gttctctatc actgataggg agtaaactcg acatacgttc tctatcactg
     2341 atagggagta aactcgacat cgttctctat cactgatagg gagtaaactc gacatacgtt
     2401 ctctatcact gatagggagt aaactcgatt gaagacgaaa gggccgaatt caagcttcgt
     2461 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2521 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2581 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2641 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2701 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2761 ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc
     2821 ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     2881 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     2941 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     3001 aaatgcgggc caggatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     3061 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3121 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3181 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3241 aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg
     3301 agagcgggcg ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct cagccgtcgc
     3361 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt
     3421 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3481 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3541 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3601 ttttcttcca tttcaggtgt cgtgaacacg ctaccgggcc accatggccg aagggagcgt
     3661 ggcaagacag cccgatctgc tgacatgcga cgacgaacca atccacattc caggagctat
     3721 ccagcctcat ggactgctgc tggctctggc cgctgacatg actatcgtgg caggctctga
     3781 taacctgcca gagctgaccg ggctggctat cggagcactg attggccggt ccgcagccga
     3841 cgtgttcgat tctgagactc acaatagact gaccatcgcc ctggctgaac ctggggctgc
     3901 agtgggagcc ccaattaccg tcggcttcac aatgcgaaag gacgctggct ttatcgggtc
     3961 ctggcaccgc catgatcagc tgatttttct ggagctggaa ccacctcaga gagacgtggc
     4021 agagccacag gctttctttc ggagaacaaa cagcgccatc aggcgcctgc aggccgctga
     4081 gactctggaa tccgcttgcg cagccgctgc acaggaagtg aggaagatca ccgggttcga
     4141 tcgcgtcatg atctacagat tcgcctcaga ctttagcgga gaagtgatcg cagaagatcg
     4201 ctgtgccgag gtcgaaagta aactgggcct gcattaccca gcctcaaccg tgcctgcaca
     4261 ggcacgacgg ctgtatacaa tcaaccccgt gcggatcatt cccgacatta attacagacc
     4321 agtgcccgtc acacctgacc tgaacccagt gactgggagg cccatcgatc tgagcttcgc
     4381 cattctgcgc agcgtgtccc ctgtccacct ggagtttatg aggaatatcg gaatgcatgg
     4441 caccatgtct atcagtattc tgagaggcga aaggctgtgg gggctgatcg tgtgccacca
     4501 tcggacacct tactatgtgg acctggacgg caggcaggct tgtgaactgg tggctcaggt
     4561 gctggcttgg cagattggcg tgatggaaga agagggcaga ggaagtcttc taacatgcgg
     4621 tgacgtggag gagaatcccg gccctatgtc tatgaccgag tacaagccca cggtgcgcct
     4681 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     4741 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     4801 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     4861 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     4921 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     4981 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     5041 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5101 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     5161 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5221 ctggtgcatg acccgcaagc ccggtgccgc cacgaacttc tctctgttaa agcaagcagg
     5281 agacgtggaa gaaaaccccg gtcctatgtc tagactggac aagagcaaag tcataaacgg
     5341 cgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca
     5401 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     5461 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     5521 cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc
     5581 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     5641 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     5701 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     5761 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     5821 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     5881 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccgg ccgacgccct
     5941 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
     6001 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa
     6061 ggatcctcta gagttaacat cgagggatca agcttatcga taatcaacct ctggattaca
     6121 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6181 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6241 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6301 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6361 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6421 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6481 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     6541 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6601 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6661 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgagacctg gaaaaacatg
     6721 gagcaatcac aagtagcaac acagcagcta ccaatgctgc ttgtgcctgg ctagaagcac
     6781 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     6841 acaaggcagc tgtagatcta gataactgat cataatcagc cataccacat ttgtagaggt
     6901 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     6961 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7021 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7081 catcaatgta tcttaacgct cgagtgcatg gatcctttgt tactttatag aagaaatttt
     7141 gagtttttgt ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt
     7201 attagtatgt aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc
     7261 tcgatataca gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac
     7321 gtcacaatat gattatcttt ctagggttaa agagctccag cttttgttcc ctttagtgag
     7381 ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7441 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7501 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7561 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7621 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7681 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7741 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7801 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7861 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7921 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7981 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8041 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8101 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8161 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8221 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8281 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8341 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8401 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8461 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8521 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8581 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8641 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8701 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8761 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8821 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8881 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8941 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9001 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9061 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9121 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9181 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9241 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9301 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9361 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9421 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9541 ttccccgaaa agtgccac
//
