LOCUS       pLz74_(CbbL-NTD-Strp_Cb 6498 bp ds-DNA     circular     06-OCT-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     mutation        1869..1871
                     /label="D341A"
     misc_feature    851..854
                     /label="Recombination Site A"
     misc_feature    2739..2742
                     /label="Recombination Site B"
     misc_feature    2275..2339
                     /label="polyPro_NTD_WK"
     misc_feature    complement(5515..5740)
                     /label="ROP/ROM"
     gene            2406..2738
                     /label="cbbS"
     misc_feature    2343..2366
                     /label="StrepII-tag"
     CDS             849..851
                     /label="CDS"
     misc_feature    complement(4537..4646)
                     /label="RNA1-for"
     primer_bind     770..789
                     /label="T7"
     rep_origin      4425..5107
                     /label="ColE1 origin"
     CDS             3470..4327
                     /label="AmpR"
     mutation        1836..1838
                     /label="E330A"
     misc_feature    2818..2858
                     /label="T7 Terminator"
     misc_feature    complement(5085..5094)
                     /label="ColE1 ORI"
     misc            1846..1860
                     /label="Primer overlap"
     gene            2367..2369
                     /label="cbbL"
ORIGIN
        1 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
       61 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
      121 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
      181 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
      241 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
      301 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
      361 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
      421 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
      481 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
      541 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
      601 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
      661 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
      721 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
      781 actataggga caccacaacg gtttccctct agaaataatt ttgtttaact ttaagaagga
      841 gatataccat gtccgcagtt aaaaagtata gtgctggtgt aaaagaatac cggcagacct
      901 attggatgcc ggaatacaca ccgttggatt ccgacatcct tgcatgcttc aaaatcaccc
      961 cacaaccggg tgttgatcgc gaagaagccg cagccgcggt tgcagcagaa tcttcaaccg
     1021 gcacatggac caccgtgtgg accgatttgc tgaccgacat ggactactac aaaggccgtg
     1081 cctatcgcat tgaagacgta cccggtgacg atgcggcatt ctatgccttt atcgcctacc
     1141 caatcgacct gttcgaagaa gggtcagttg ttaacgtgtt tacctcactg gttggtaacg
     1201 tgttcggctt caaagcggta cgcggcctgc gtctggaaga tgttcgcttc ccactcgcct
     1261 acgttaaaac ctgtggcggc ccaccgcacg gtattcaagt cgaacgcgac aagatgaaca
     1321 aatatggtcg cccactgttg ggttgcacca tcaagccaaa acttggtttg tctgcgaaaa
     1381 actacggccg tgccgtatac gagtgcctcc gtggcggcct cgacttcact aaagatgatg
     1441 aaaacatcaa ttctcagccg ttcatgcgct ggcgcgatcg cttcttgttc gtacaagacg
     1501 cgaccgaaac tgctgaagcc caaaccggcg aacgcaaagg ccattacctc aacgtaacgg
     1561 cgccaactcc tgaagaaatg tacaagcgcg cagaattcgc caaagaaatt ggcgcgccaa
     1621 tcattatgca cgactacatc accggtggct tcacggccaa cactggcttg gccaagtggt
     1681 gtcaagacaa cggcgtactg ctgcacatcc accgtgcgat gcatgcggtt atcgaccgta
     1741 acccgaacca cggtattcac ttccgtgttc tgaccaagat tctgcgtttg tcgggtggcg
     1801 atcacctgca caccggtacc gttgtcggca aactggaagg cgaccgtgcc tctactctgg
     1861 gctggattga tttgctccgc gaatcgttta tccctgaaga tcgctcgcgc ggtatcttct
     1921 tcgatcaaga ctggggttca atgccaggcg tattcgctgt ggcctctggt ggtattcacg
     1981 tatggcacat gcctgcgctg gttaacatct ttggtgacga ctctgtcctc caattcggtg
     2041 gcggtacgct gggtcatcca tggggcaacg ctgccggtgc tgctgccaac cgtgttgctc
     2101 tggaagcctg cgtagaagcg cgtaaccaag gccgcgatat cgaaaaagaa ggcaaagaaa
     2161 ttctgactgc tgctgcacag cacagcccag aactgaagat tgccatggaa acttggaaag
     2221 agatcaaatt cgaatttgac actgtcgaca aactcgacac tcaaaatcgG AGCagcGGAC
     2281 GTGATTTAGC TCGCGCTCGT CGTGAGGCAT TAAGTCAACA GGGTAAAGCA GCCGTCGGGT
     2341 CCTGGAGCCA CCCGCAGTTC GAAAAGtgat ccctcgtacc acacaacata ctaaggtgag
     2401 taaccatggc tgaaatgcag gattacaagc aaagcctcaa atatgagact ttctcttatc
     2461 ttccacccat gaacgcggaa cgcatccgcg ctcaaatcaa gtacgcaatt gctcaaggct
     2521 ggagccccgg cattgagcac gtagaagtga aaaactccat gaaccaatat tggtacatgt
     2581 ggaaacttcc cttcttcggc gaacaaaatg tcgacaacgt gttggctgaa attgaagcgt
     2641 gtcgtagtgc gtatccaaca caccaggtca aactggtggc ttatgacaac tatgcgcaaa
     2701 gcttaggtct ggccttcgtg gtctaccgcg gcaactaaag cggttaagat ccggctgcta
     2761 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     2821 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
     2881 gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc
     2941 gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg
     3001 catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg
     3061 tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct
     3121 acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac
     3181 ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg
     3241 ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt
     3301 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     3361 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3421 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3481 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3541 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3601 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3661 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     3721 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3781 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3841 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3901 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3961 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     4021 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4081 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4141 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggctctc gcggtatcat
     4201 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4261 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4321 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4381 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4441 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4501 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4561 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4621 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4681 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4741 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4801 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4861 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4921 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4981 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5041 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5101 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5161 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5221 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     5281 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
     5341 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     5401 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     5461 tgctcccggc atccgcttac agacaagctg tgaccgcctc cgggagctgc atgtgtcaga
     5521 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     5581 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     5641 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     5701 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     5761 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     5821 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     5881 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     5941 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     6001 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     6061 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     6121 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
     6181 acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
     6241 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     6301 ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
     6361 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
     6421 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
     6481 cggtccagtg atcgaagt
//