LOCUS       pBz110_(His-CTD)        5468 bp ds-DNA     circular     21-JUN-2019
DEFINITION  .
KEYWORDS    "creator:Savage" "marker:AmpR"                                     
FEATURES             Location/Qualifiers
     rep_origin      1164..1846
                     /label="ColE1 origin"
     misc_feature    complement(1824..1833)
                     /label="ColE1 ORI"
     CDS             209..1066
                     /label="AmpR"
     CDS             4109..4937
                     /label="carboxysome shell polypeptide"
     CDS             4086..4087
                     /label="CDS"
     misc_feature    5025..5065
                     /label="T7 Terminator"
     misc_feature    4946..4949
                     /label="Recombination Site B"
     misc_feature    complement(2254..2479)
                     /label="ROP/ROM"
     misc_feature    4109..4945
                     /label="TN_Carb_Seq_Cloning"
     misc_feature    complement(1276..1385)
                     /label="RNA1-for"
     gene            4109..4937
                     /label="csoS2"
     primer_bind     4007..4026
                     /label="T7"
     misc_feature    4109..4945
                     /label="Cloned to pSAV038"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtggctctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca
     2101 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     2161 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     2221 gacaagctgt gaccgcctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     2281 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt
     2341 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
     2401 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
     2461 aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga
     2521 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg
     2581 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta
     2641 atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca
     2701 taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga
     2761 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct
     2821 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc
     2881 tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc
     2941 gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt
     3001 gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt
     3061 tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg
     3121 caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca
     3181 tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag
     3241 gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct
     3301 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
     3361 ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc
     3421 cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac
     3481 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
     3541 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
     3601 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
     3661 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt
     3721 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc
     3781 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc
     3841 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc
     3901 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
     3961 cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact
     4021 atagggacac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat
     4081 ataccatGTC CCACCATCAT CATCATCATa cgagcacccc agagcccgaa gctcaatcaa
     4141 ctgaacaatc attgacctgt gaaggacaaa ttattagcgg cacttcagtt gacgccagtg
     4201 atttggtcac aggaaatgaa atcggtgaac agcaactcat cagcggtgac gcctatgttg
     4261 gcgcgcagca gacaggttgc cttcccacta gtccacgctt caaccaaact ggcaatgttc
     4321 agtcaatggg ttttaagaac accaatcagc cagaacaaaa ctttgcacca ggtgaagtaa
     4381 tgcctactga ctttagtatt caaaccccag ctcgctcggc tcagaatcgc attacaggta
     4441 acgacattgc gccctcaggt cgcattacag gccctggtat gctggcaacc ggcttgatta
     4501 caggaacccc cgaattcagg cacgctgcgc gcgagttggt tggttctcca caacccatgg
     4561 caatggccat ggccaaccgt aataaggctg ctcaagcacc tgttgtgcag ccagaagtgg
     4621 ttgcaactca ggaaaagcct gagttggtat gtgcaccaag aagcgatcaa atggatcgtg
     4681 tgagtggcga aggcaaagaa cgttgccaca tcactggcga tgactggtca gttaacaagc
     4741 acatcaccgg tacagccggt caatgggcga gtggtcgcaa cccttccatg cgcggtaatg
     4801 cgcgtgtggt cgaaaccagc gcgtttgcca atcgcaatgt gccaaaacct gaaaagccgg
     4861 gctccaagat cacgggcagt agtggtaatg acacccaagg tagtctgatc acttactccg
     4921 gcggcgcgcg cggttgatta agtaaagcgg ttaagatccg gctgctaaca aagcccgaaa
     4981 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     5041 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga
     5101 cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga
     5161 ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca
     5221 tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga
     5281 tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg
     5341 tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc
     5401 gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa
     5461 catgagaa
//