LOCUS       pLz54_(fishing_NTD_>_20 4949 bp ds-DNA     circular     13-FEB-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(2659..2768)
                     /label="RNA1-for"
     misc_feature    complement(3637..3862)
                     /label="ROP/ROM"
     CDS             532..549
                     /label="6xHis"
     CDS             520..522
                     /label="CDS"
     misc_feature    complement(3207..3216)
                     /label="ColE1 ORI"
     protein         763..822
                     /label="synth NTD"
     CDS             1592..2449
                     /label="AmpR"
     primer_bind     441..460
                     /label="T7"
     misc_feature    940..980
                     /label="T7 Terminator"
     rep_origin      2547..3229
                     /label="ColE1 origin"
ORIGIN
        1 cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag
       61 tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga
      121 aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
      181 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
      241 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
      301 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
      361 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
      421 agatctcgat cccgcgaaat taatacgact cactataggg acaccacaac ggtttccctc
      481 tagaaataat tttgtttaac tttaagaagg agatatacca tgtccTGGAA Gcaccatcac
      541 catcaccatg aaaacctgta cttccagtcc GCAGCTGTTG GTGGGGGATC TGGAGGTGGG
      601 TCTGGTGGAC CTCCCCCGCC TCCACCGCCA CCGCCAGCTC CTGCACCTGC GCCTGCTCCT
      661 CCGCCACCCC CTCCACCGCC ACCTGCTCCT GCTCCTGCTC CGGCACCACC ACCTCCGCCT
      721 CCGCCACCTG GAGGTGGATC AGGAGGTGGT TCAGGTGGAG GAGGGACAGG GTCCACAGGA
      781 TCCGGATCGT CGTCTTCCGG CTCCGGAACA TCTGGGACAG GCGGTGGCGG TAGCGGAGGT
      841 GGTAGTGGTG GAAGCGgtta AGCGgttaag atccggctgc taacaaagcc cgaaaggaag
      901 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      961 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt
     1021 gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg
     1081 cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac
     1141 gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc
     1201 cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg
     1261 gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag
     1321 caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga
     1381 gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1441 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1501 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1561 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1621 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1681 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1741 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1801 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     1861 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1921 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1981 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     2041 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     2101 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     2161 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     2221 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     2281 tgataaatct ggagccggtg agcgtggctc tcgcggtatc attgcagcac tggggccaga
     2341 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     2401 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2461 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2521 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2581 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2641 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2701 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2761 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2821 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2881 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2941 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3001 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3061 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3361 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     3421 gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc
     3481 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     3541 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     3601 acagacaagc tgtgaccgcc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     3661 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     3721 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     3781 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     3841 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     3901 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     3961 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     4021 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     4081 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     4141 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     4201 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     4261 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     4321 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     4381 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     4441 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     4501 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     4561 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     4621 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     4681 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     4741 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     4801 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     4861 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     4921 cgtcgcgctc cagcgaaagc ggtcctcgc
//