LOCUS       pLz56_(fishing_NTD_>_MR 5039 bp ds-DNA     circular     13-FEB-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    645..743
                     /label="RADAR repeat 4"
     primer_bind     272..291
                     /label="T7"
     misc_feature    594..743
                     /label="Cheryl's M-repeat 1"
     misc_feature    861..901
                     /label="T7 Terminator"
     rep_origin      2468..3150
                     /label="ColE1 origin"
     CDS             1513..2370
                     /label="AmpR"
     CDS             363..380
                     /label="6xHis"
     misc_feature    complement(2580..2689)
                     /label="RNA1-for"
     CDS             351..353
                     /label="CDS"
     misc_feature    complement(3128..3137)
                     /label="ColE1 ORI"
     misc_feature    complement(3558..3783)
                     /label="ROP/ROM"
ORIGIN
        1 taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc
       61 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
      121 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
      181 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
      241 gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg gacaccacaa
      301 cggtttccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgtccTGGA
      361 AGcaccatca ccatcaccat gaaaacctgt acttccagtc cGCAGCTGTT GGTGGGGGAT
      421 CTGGAGGTGG GTCTGGTGGA CCTCCCCCGC CTCCACCGCC ACCGCCAGCT CCTGCACCTG
      481 CGCCTGCTCC TCCGCCACCC CCTCCACCGC CACCTGCTCC TGCTCCTGCT CCGGCACCAC
      541 CACCTCCGCC TCCGCCACCT GGAGGTGGAT CAGGAGGTGG TTCAGGTGGA GGAaaggttg
      601 aaaccggtca caccctatcc ggcggatctg ttacgggcac gcaagtggat cgtaaatctc
      661 atgtgactgg aaacgagccg ggcacttgcc gagcagtcac gggcaccgag tacgtaggta
      721 ctgagcaatt cacctctttt tgcGGTGGCG GTAGCGGAGG TGGTAGTGGT GGAAGCGgtt
      781 aAGCGgttaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      841 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      901 gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt
      961 agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg
     1021 acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca
     1081 cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta
     1141 ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga
     1201 gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac
     1261 taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag
     1321 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     1381 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1441 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1501 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1561 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1621 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1681 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1741 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1801 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1861 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1921 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1981 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2041 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2101 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2161 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2221 gagcgtggct ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2281 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2341 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     2401 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2461 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2521 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2581 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2641 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     2701 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2761 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2821 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2881 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2941 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3001 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3061 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3181 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3241 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3301 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3361 caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     3421 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     3481 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgc
     3541 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     3601 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     3661 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     3721 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     3781 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     3841 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     3901 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     3961 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     4021 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     4081 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     4141 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     4201 gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct
     4261 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     4321 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg
     4381 gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag
     4441 acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg
     4501 gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg
     4561 agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc
     4621 aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag
     4681 cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata
     4741 atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg
     4801 gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag
     4861 cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata
     4921 aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg
     4981 actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcat
//