LOCUS       pLz58_(fishing_NTD_>_CT 4985 bp ds-DNA     circular     13-FEB-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             359..376
                     /label="6xHis"
     CDS             590..685
                     /label="carboxysome shell polypeptide"
     primer_bind     268..287
                     /label="T7"
     CDS             1455..2312
                     /label="AmpR"
     misc_feature    590..685
                     /label="Cloned to pSAV038"
     rep_origin      2410..3092
                     /label="ColE1 origin"
     gene            590..685
                     /label="csoS2"
     misc_feature    590..685
                     /label="TN_Carb_Seq_Cloning"
     misc_feature    complement(3070..3079)
                     /label="ColE1 ORI"
     misc_feature    complement(3500..3725)
                     /label="ROP/ROM"
     misc_feature    803..843
                     /label="T7 Terminator"
     CDS             347..349
                     /label="CDS"
     misc_feature    complement(2522..2631)
                     /label="RNA1-for"
ORIGIN
        1 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
       61 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
      121 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      181 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      241 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tatagggaca ccacaacggt
      301 ttccctctag aaataatttt gtttaacttt aagaaggaga tataccatgt ccTGGAAGca
      361 ccatcaccat caccatgaaa acctgtactt ccagtccGCA GCTGTTGGTG GGGGATCTGG
      421 AGGTGGGTCT GGTGGACCTC CCCCGCCTCC ACCGCCACCG CCAGCTCCTG CACCTGCGCC
      481 TGCTCCTCCG CCACCCCCTC CACCGCCACC TGCTCCTGCT CCTGCTCCGG CACCACCACC
      541 TCCGCCTCCG CCACCTGGAG GTGGATCAGG AGGTGGTTCA GGTGGAGGAg tgccaaaacc
      601 tgaaaagccg ggctccaaga tcacgggcag tagtggtaat gacacccaag gtagtctgat
      661 cacttactcc ggcggcgcgc gcggtGGTGG CGGTAGCGGA GGTGGTAGTG GTGGAAGCGg
      721 ttaAGCGgtt aagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      781 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      841 ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc
      901 gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc
      961 ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag
     1021 cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag
     1081 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat
     1141 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa
     1201 actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa
     1261 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     1321 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1381 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1441 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1501 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1561 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1621 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1681 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1741 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1801 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1861 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1921 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1981 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     2041 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2101 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2161 gtgagcgtgg ctctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2221 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2281 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2341 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2401 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2461 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2521 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2581 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2641 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2701 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2761 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2821 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2881 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2941 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3001 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3061 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3121 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3181 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3241 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3301 cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     3361 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     3421 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3481 gcctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     3541 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     3601 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     3661 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     3721 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     3781 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     3841 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
     3901 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
     3961 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     4021 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     4081 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     4141 acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg
     4201 ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt
     4261 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
     4321 cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc
     4381 agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg
     4441 cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg
     4501 cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
     4561 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
     4621 agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga
     4681 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
     4741 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     4801 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     4861 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     4921 tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca
     4981 ttagg
//