LOCUS       Exported                4215 bp ds-DNA    circular SYN 05-MAR-2020
DEFINITION  Expression vector pTNT(TM), complete sequence.
ACCESSION   AF479322
VERSION     .
KEYWORDS    pTNT-pT7-TetR-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   English J.
  TITLE     pTNT(TM) Vector Technical Bulletin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   English J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2002) Technical Writing, Promega Corporation, 2800
            Woods Hollow Road, Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 4215)
  AUTHORS   Unspecified
  TITLE     Direct Submission
  JOURNAL   Exported Mar 9, 2020 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1457..1456
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1463..75
                     /organism="Expression vector pTNT(TM)"
                     /mol_type="genomic DNA"
                     /note="designed for expressing cloned genes in vivo or in 
                     vitro"
                     /db_xref="taxon:187378"
     regulatory      35..53
     regulatory      59..75
     RBS             112..119
     CDS             128..748
                     /codon_start=1
                     /note="TetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             749..1456
                     /codon_start=1
                     /note="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    1494..1523
                     /note="synthetic poly(A)30 region"
     regulatory      1524..1571
     misc_feature    1650..2105
                     /note="phage f1 region"
     CDS             2542..3402
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /note="AmpR"
                     /note="beta-lactamase"
                     /db_xref="GI:18997125"
                     /protein_id="AAL83304.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cacatggctc gacagatctt aaggctagag tacttaatac gactcactat aggctagcat
       61 ttaggtgaca ctataagcac atcagcagga cgcactgacc gaattcatta aagaggagaa
      121 aggtaccatg tccagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa
      181 tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca
      241 gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga
      301 gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt
      361 tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
      421 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
      481 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg
      541 gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag
      601 ggaaacacct actactgata gtatgccgcc attattacga caagctatcg aattatttga
      661 tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga
      721 aaaacaactt aaatgtgaaa gtgggtctat ggtgagcaag ggcgaggagg ataacatggc
      781 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      841 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      901 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      961 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     1021 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     1081 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     1141 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1201 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     1261 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     1321 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     1381 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     1441 ggacgagctg tacaagtaat aatggtacct ctagagtcga cccgggcggc cgcaaaaaaa
     1501 aaaaaaaaaa aaaaaaaaaa aaactagcat aaccccttgg ggcctctaaa cgggtcttga
     1561 ggggtttttt ggatccgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     1621 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc
     1681 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     1741 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     1801 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     1861 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     1921 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     1981 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     2041 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     2101 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     2161 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     2221 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     2281 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     2341 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     2401 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2461 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2521 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2581 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2641 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2701 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2761 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2821 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2881 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2941 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3001 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3061 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3121 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3181 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3241 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3301 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3361 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3421 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3481 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3541 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3601 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3661 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3721 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3781 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3901 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3961 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4021 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4081 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4141 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4201 tgctggcctt ttgct
//
