LOCUS       pUC19                   7249 bp    DNA     circular SYN 24-Aug-2018
DEFINITION  Cloning vector pUC19, complete sequence.
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     See also GenBank accession L09137.
FEATURES             Location/Qualifiers
     source          join(<4987..>7249,<1..>416)
                     /mol_type="other DNA"
                     /organism="Cloning vector pUC19"
                     /note="/dnas_title=""pUC19"" [Split]"
     gene            complement(join(146..>417,<4987..5032))
                     /gene="lacZalpha"
                     /note="/dnas_title=""lacZalpha"" [Split]"
     misc_feature    join(396..>417,<4987..5015)
                     /gene="lacZalpha"
                     /note=" [Split]"
     frag            418..4986
                     /date="09-Feb-2018"
                     /note="1 to 4569 of IR7f_QF2_plasmid"
     frag            <418..>1416
                     /date="08-Feb-2018"
                     /note="1 to 2422 of Ir7f_gDNA_homologyarms"
     variation       418..1416
                     /note="upstream_arm"
     variation       496..498
                     /note="Start codon"
     variation       complement(496..>1416)
                     /note="exon1 [Split]"
     variation       1407
                     /note="C>T"
     variation       1410
                     /note="T>C"
     variation       1413
                     /note="A>G"
     variation       1416
                     /note="C>T"
     misc_feature    1417..1473
                     /label="T2A"
                     /note="T2A"
                     /note="; /ApEinfo_fwdcolor=""pink"
                     /note="; /ApEinfo_revcolor=""pink"
                     /note="; /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0
                     -1} {} 0} width 5 offset 0"""
     frag            1417..3985
                     /date="08-Feb-2018"
                     /note="3218 to 5786 of pTS1_Dop1R1_del_"
     variation       1417..1419
                     /note="T2A infame"
     misc_feature    1474..2529
                     /label="QF2"
                     /note="QF2"
                     /note="; /ApEinfo_fwdcolor=""pink"
                     /note="; /ApEinfo_revcolor=""pink"
                     /note="; /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0
                     -1} {} 0} width 5 offset 0"""
     misc_feature    2534..2790
                     /label="SV40 Term"
                     /note="SV40 Term"
                     /note="; /ApEinfo_fwdcolor=""pink"
                     /note="; /ApEinfo_revcolor=""pink"
                     /note="; /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0
                     -1} {} 0} width 5 offset 0"""
     misc_feature    2791..3036
                     /label="3xP3"
                     /note="3xP3"
                     /note="; /ApEinfo_fwdcolor=""pink"
                     /note="; /ApEinfo_revcolor=""pink"
                     /note="; /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0
                     -1} {} 0} width 5 offset 0"""
     misc_feature    3066..3743
                     /label="dsRed"
                     /note="dsRed"
                     /note="; /ApEinfo_fwdcolor=""pink"
                     /note="; /ApEinfo_revcolor=""pink"
                     /note="; /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0
                     -1} {} 0} width 5 offset 0"""
     misc_feature    3356
                     /label="G>T mutation"
                     /note="; /ApEinfo_fwdcolor=""cyan"
                     /note="; /ApEinfo_revcolor=""#27f300"
                     /note="; /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0
                     -1} {} 0} width 5 offset 0"""
     misc_feature    3758..3985
                     /label="SV40"
                     /note="SV40"
                     /note="; /ApEinfo_fwdcolor=""pink"
                     /note="; /ApEinfo_revcolor=""pink"
                     /note="; /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0
                     -1} {} 0} width 5 offset 0"""
     variation       complement(<3986..4478)
                     /note=" [Split]"
     variation       3986..4986
                     /note="downstream_arm"
     frag            3986..4986
                     /date="08-Feb-2018"
                     /note="2423 to 3423 of Ir7f_gDNA_homologyarms"
     variation       <3986..3988
                     /note=" [Split]"
     variation       3986
                     /note="cut"
     variation       3989..3991
                     /note="PAM"
     variation       complement(4538..>4986)
                     /note="exon2 [Split]"
     -10_signal      complement(5077..5082)
                     /note="Plac promoter (drives lacZalpha; counter-clockwise);
                     TATGTT"
     -35_signal      complement(5101..5106)
                     /note="Plac promoter (drives lacZalpha; counter-clockwise);
                     TTTACA"
     protein_bind    complement(5126..5138)
                     /bound_moiety="catabolite activator protein (CAP)"
                     /note="CAP protein binding site"
     misc_RNA        complement(5430..5982)
                     /note="RNAII transcript (complementary strand)"
     rep_origin      complement(5430..6018)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     -35_signal      5836..5841
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      5858..5863
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        5872..5979
                     /note="RNAI transcript"
     -10_signal      complement(5992..5997)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(6013..6018)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     gene            complement(6189..7049)
                     /gene="bla"
                     /note="/dnas_title=""bla"""
     sig_peptide     complement(6981..7049)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved off
                     to form the mature beta-lactamase protein."
                     /note="/dnas_title=""bla"""
     -10_signal      complement(7093..7098)
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(7114..7119)
                     /note="bla promoter (counter-clockwise); TTCAAA"
BASE COUNT  1791 A  1915 C  1815 G  1728 T  0 OTHER
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCCGGGTGA
      421 CTGGCTGATT AGCTCATCCT ATATAAGAAT AATCCTCAAT CTGTTGGACT ACATCGCACA
      481 CCAGATCCGC GTACCATGAG ATTCCTCACA GTACTCGTAA CCTTAAGCGT GGTTTCGTGC
      541 GAGGCATCGC ACTTACTCCT GCCCAAGAAA TCTTCCACCG ATGACTTCAG CAACGCCATC
      601 ATTGCCATCC TGAGGCGACA TTTCGTTTCA AATCACGCTT CAACGATGAT CACCCAATCC
      661 GCCAAGGTAG ACACCTACAC GGAGTATCTC CAAATAGACA TCCTCGATCG CATACTGCTC
      721 CGAACGTCCG GTGAAATCGC CTACGTCTTC AGCACTCCGA TCCGACCGCG ACGACCTCGG
      781 TTCTTCAACA TACTGCTGTT CGACGGCTAC CTAGCATTCC GTAGCATTCT GCGCCAGCTT
      841 CACCCAAACA AGTACGACTT CTCTGGATAT TATCTGCTGA TCTTGACGGC ACCACGACCC
      901 GTGTTCAACA GAACCATTCA GCAGATGTTC ATTGATCTGT GGTCGTACAA TATCGTCAAC
      961 GTGGTGCTGG CAGCCTCCAG TATTGTCGGT TCGAAAAGCC CTGAAGGTAC ACAGTTGTTC
     1021 ACCTACTTCC CATACGTTGA ACATCACTGT GAACATGCCC ACCCCGTATT ACTATATAAC
     1081 TTGACCCCGT CCACGAAGCT CGACAAATCC GTGAACTTGT TTCCGCCGAA GCTTTCCAAC
     1141 TTTCACGGTT GCCCGATCCG TATCGCTGCC ATCGACTACC CACCGTACAC AATGATCGAA
     1201 TGGATTGGAG GTCGCCGAAG ACTACGTGGA ATAGAAGGCG ACATGCTGAA CATGATTTCC
     1261 TCGGAGCTGA ACTTCACGTA CGAGGTAGTG GAGATGACGG GCGACGATCG TTGGGGACAA
     1321 ATCTACGGGA ACGGAACGTT CACTGGAGCG ATAAAGTTGG TGGTTGAAGG CTCTGTCAAC
     1381 ATGTCGACCG GGTGTTTCTC GATGCGTGGC GAGCGTGGAG AGGGCAGAGG AAGTCTTCTA
     1441 ACATGCGGTG ACGTGGAGGA GAATCCCGGC CCTATGCCAC CCAAGCGCAA AACGCTTAAC
     1501 GCTGCGGCTG AGGCTAACGC TCATGCCGAC GGACACGCCG ACGGAAACGC CGACGGACAC
     1561 GTGGCCAATA CGGCCGCGTC CTCGAATAAT GCGAGGTTCG CTGATCTCAC TAACATCGAT
     1621 ACTCCGGGTC TGGGACCCAC AACTACGACC CTGCTCGTGG AACCAGCACG CTCAAAGCGT
     1681 CAACGAGTGT CCCGCGCATG CGACCAGTGC CGTGCAGCCC GAGAGAAATG CGACGGAATA
     1741 CAGCCTGCGT GTTTCCCGTG CGTTTCCCAG GGAAGGTCCT GCACTTATCA GGCTTCGCCG
     1801 AAAAAGAGGG GAGTTCAAAC CGGTTATATT CGTACGCTGG AGCTCGCCCT CGCCTGGATG
     1861 TTTGAAAATG TCGCGCGTTC CGAAGATGCC TTGCATAACC TCCTCGTCCG TGACGCCGGA
     1921 CAAGGATCAG CTCTGCTCGT TGGTAAAGAT TCGCCGGCTG CCGAGCGACT CCATGCCCGT
     1981 TGGGCTACTA GCCGTGTCAA TAAGAGCATT ACCCGCCTCC TCCGTCAGTT GGAGCTCCCT
     2041 CCTACCGCCA CGGCTACGGC CTCGATAATG CCGCACGTGA TGGAGCAGCC TCTCAGTACC
     2101 AGCATTAACC CCGTCAACGA CCGCTTCAAC GGTATTCCCA ACCCCACTCC GTATAACTCC
     2161 GATGCAGCTC TCGATGCTAT CACTCAGACC AACGATTATG GAAGCGTAAA TACACATGGT
     2221 ATCCTCTCTA CTTACCCGCC ACCGGCTACG CACCTTAATG AAGCTTCCGT CGCTCTCGCT
     2281 CCCGGTGGCG CCCCCCCCCG ACCGCCTCCT CCGTATGTTG ACAGCACGAC CAATCACCCG
     2341 CCGTACCACT CGAATCTGGT TCCAATGGCG AACTTTGGTT ACTCGACCGT TGATTACGAT
     2401 GCCATGGTTG ACGATTTGGC TAGCATTGAA TACACGGACG CTGTGGATGT CGACCCACAG
     2461 TTTATGACCA ATCTGGGATT CGTTCCTGGA TGTAACTTCT CCGACATTAA TACATACGAA
     2521 CAGTGATGAC GTCTATCGAT ACCGTCGACT AAAGCCAAAT AGAAAATTAT TCAGTTCCTG
     2581 GCTTAAGTTT TTAAAAGTGA TATTATTTAT TTGGTTGTAA CCAACCAAAA GAATGTAAAT
     2641 AACTAATACA TAATTATGTT AGTTTTAAGT TAGCAACAAA TTGATTTTAG CTATATTAGC
     2701 TACTTGGTTA ATAAATAGAA TATATTTATT TAAAGATAAT TGCGTTTTTA TTGTCAGGGA
     2761 GTGAGTTTGC TTAAAAACTC GTTTAGATCC AATTCGAGCT CGCCCGGGGA TCTAATTCAA
     2821 TTAGAGACTA ATTCAATTAG AGCTAATTCA ATTAGGATCC AAGCTTATCG ATTTCGAACC
     2881 CTCGACCGCC GGAGTATAAA TAGAGGCGCT TCGTCTACGG AGCGACAATT CAATTCAAAC
     2941 AAGCAAAGTG AACACGTCGC TAAGCGAAAG CTAAGCAAAT AAACAAGCGC AGCTGAACAA
     3001 GCTAAACAAT CGGGGTACCG CTAGAGTCGA CGGTACCGCG GGCCCGGGAT CCACCGGTCG
     3061 CCACCATGGC CTCCTCCGAG AACGTCATCA CCGAGTTCAT GCGCTTCAAG GTGCGCATGG
     3121 AGGGCACCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC CGCCCCTACG
     3181 AGGGCCACAA CACCGTGAAG CTGAAGGTGA CCAAGGGCGG CCCCCTGCCC TTCGCCTGGG
     3241 ACATCCTGTC CCCCCAGTTC CAGTACGGCT CCAAGGTGTA CGTGAAGCAC CCCGCCGACA
     3301 TCCCCGACTA CAAGAAGCTG TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC GTGATGAACT
     3361 TCGAGGACGG CGGCGTGGCG ACCGTGACCC AGGACTCCTC CCTGCAGGAC GGCTGCTTCA
     3421 TCTACAAGGT GAAGTTCATC GGCGTGAACT TCCCCTCCGA CGGCCCCGTG ATGCAGAAGA
     3481 AGACCATGGG CTGGGAGGCC TCCACCGAGC GCCTGTACCC CCGCGACGGC GTGCTGAAGG
     3541 GCGAGACCCA CAAGGCCCTG AAGCTGAAGG ACGGCGGCCA CTACCTGGTG GAGTTCAAGT
     3601 CCATCTACAT GGCCAAGAAG CCCGTGCAGC TGCCCGGCTA CTACTACGTG GACGCCAAGC
     3661 TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA GCAGTACGAG CGCACCGAGG
     3721 GCCGCCACCA CCTGTTCCTG TAGCGGCCGC GACTCTAGAT CATAATCAGC CATACCACAT
     3781 TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA
     3841 AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA
     3901 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT
     3961 TGTCCAAACT CATCAATGTA TCTTATGTCG GTGATGAGGT CCAGCGCGAG TTACTACACG
     4021 ATCAAGATGA TGTTTGCCGT TCCACCGGGA AGGCCCTACA CGGCGTTCGA GAAGATGTTC
     4081 CGACCGTTTA GTGCTGTCAC CTGGATGATG GTGACCTTGT ACACGTTGGC TGGCGCTGGA
     4141 GTGATCCTCG TGTTGCAGTT CTACCCGTGG AGTGTGCGGA ACTTTGTCTA CGGTCGGAGT
     4201 ACGGGGATGG CTTTCATGAA CTTTATGAAT ATCTTCTTCG GTGGTGCGAT GAGTCGAACT
     4261 CCGGGGAGAA ATTTCGCGAG GGCTTTGCTG TTTTTGTGGT TGCTTCAGTG TTTCGTAATG
     4321 CGGACTCTGT ACCAGGGATC GCTGTTTGAG TACCTGCAGC AATCGAAGAA TTTCAGCCAC
     4381 GTGGATTCGC TCAAGCGCAT CGAGGCCGAG AAGATGCCGT ACATAATGTC GCAAGGTAAT
     4441 ATCATGTTCG TCAGAGATAT TTCCAGTATT CGCAAGAGGT AACCCGAAAA CCCTGAGATT
     4501 AGTTCCAAAA ATTTCAATAA GAGTTCATAA TTTCTAGGAC AACATTGATC CCAGACAGGA
     4561 CCGAGGACCG CTTCGAGATC TTCAGGCAAC AAAGCGTGAA TTACCTGCAG GTGGCCATGC
     4621 TAACGGCGTT CGACCACCTG GCGGCCTTTA ACGAGCGGTT CTACAAACGA GGCATCGTAC
     4681 ATGTGGCGAA GGACGTGCTC ATAACTTCGC CGATCTGCTT TTACTATCCG CAGAAATCCT
     4741 GTCTGGCTGC ACGGTTTGAC GTGGAAATCG GGAGGATTCA GCGAGCCGGT CTGATCGAAC
     4801 ATTGGCTGCA CGGATACATC GGATATCGGT TCTTCCGGAA GCCGAAGACT CATCACCACA
     4861 TTCCGATGGT GCTGACCAAT GAGCACTTGT TGGGATGCTA TGAAACGTGG CTTGCTATGT
     4921 TGGTGCTGGC ATGTGGGCTG TTTGCAATGG AGGTGCTGTC GGAGAGGGTG CACATTTTGA
     4981 GGCGGGCTAG AGTCGACCTG CAGGCATGCA AGCTTGGCGT AATCATGGTC ATAGCTGTTT
     5041 CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG
     5101 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG
     5161 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG
     5221 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC
     5281 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
     5341 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
     5401 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
     5461 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
     5521 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
     5581 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
     5641 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
     5701 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
     5761 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
     5821 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT
     5881 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
     5941 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
     6001 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     6061 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     6121 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     6181 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     6241 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     6301 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA
     6361 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     6421 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     6481 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     6541 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     6601 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     6661 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     6721 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     6781 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     6841 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     6901 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     6961 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     7021 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     7081 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     7141 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT
     7201 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTC
//
