LOCUS       pHB-4                   6730 bp ds-DNA     circular     17-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    5352..5370
                     /label="N31"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1692..1709
                     /label="Seq P R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1505..1505
                     /label="G"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3357..4538
                     /label="phaA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5529..5547
                     /label="N23"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1401..1436
                     /label="Rho Independed Terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             1317..1382
                     /label="thrL CDS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1369..1370
                     /label="MM"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1371..1380
                     /label="stem1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    5765..6424
                     /label="cam"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1319..1319
                     /label="SE"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1128..1298
                     /label="PthrC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1381..1382
                     /label="KW"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     gene            1317..1382
                     /label="thrL -open leader peptide"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1..19
                     /label="N22"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1406..1415
                     /label="stem1rc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1298..1298
                     /label="SE"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4613..5351
                     /label="phaB"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1464..1504
                     /label="RBS1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1401..1414
                     /label="stem2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1505..5351
                     /label="RephaCAB"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1108..1126
                     /label="N21"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1505..3272
                     /label="phaC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    5371..5528
                     /label="pheA terminator fragment"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    73..985
                     /label="PAC(P15A\ori\)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1300..1315
                     /label="SE-assembly"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1354..1399
                     /label="PthrC3 mutation f"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1128..1462
                     /label="PthrC3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5351..5351
                     /label="T"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1421..1433
                     /label="stem2 rc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1443..1463)
                     /label="PthrCT1-R"
                     /note="sequence: aggttgttacctcgttacctt"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          1127..1147
                     /label="PthrC-F"
                     /note="sequence: Gagcttttcattctgactgca"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 tcACACAACA TAGCCACggt gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac
       61 cgccggacat cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt
      121 gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata
      181 tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
      241 tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga
      301 tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc
      361 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
      421 aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg
      481 gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca
      541 gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg
      601 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag
      661 caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc
      721 ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct
      781 cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt
      841 tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt
      901 aatcagataa aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
      961 gtcagcccca tacgatataa gttgtaattc tcatgtttga cagcttatca cccagtcctg
     1021 ctcgcttcgc tacttggcca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     1081 agcccgatct tccccatcgg tgatgtctcC CTCGACTCAC ACTTggGagc ttttcattct
     1141 gactgcaacg ggcaatatgt ctctgtgtgg attaaaaaaa gagtgtctga tagcagcttc
     1201 tgaactggtt acctgccgtg agtaaattaa aattttattg acttaggtca ctaaatactt
     1261 taaccaatat aggcatagcg cacagacaga taaaaattac agagtacaca acatccatga
     1321 aacgcattag caccaccatt accaccacca tcaccattac cacaggtaac ggtgcgggct
     1381 gacgcgtaca ggaaacacag aaaaaagccc gcacctgaca gtgcgggctt ttttttcgac
     1441 caaaggtaac gaggtaacaa ccTagaaata attTTGTTTA ACTTTAAGAA GGagatatac
     1501 atatGgcgac cggcaaaggc gcggcagctt ccacgcagga aggcaagtcc caaccattca
     1561 aggtcacgcc ggggccattc gatccagcca catggctgga atggtcccgc cagtggcagg
     1621 gcactgaagg caacggccac gcggccgcgt ccggcattcc gggcctggat gcgctggcag
     1681 gcgtcaagat cgcgccggcg cagctgggtg atatccagca gcgctacatg aaggacttct
     1741 cagcgctgtg gcaggccatg gccgagggca aggccgaggc caccggtccg ctgcacgacc
     1801 ggcgcttcgc cggcgacgca tggcgcacca acctcccata tcgcttcgct gccgcgttct
     1861 acctgctcaa tgcgcgcgcc ttgaccgagc tggccgatgc cgtcgaggcc gatgccaaga
     1921 cccgccagcg catccgcttc gcgatctcgc aatgggtcga tgcgatgtcg cccgccaact
     1981 tccttgccac caatcccgag gcgcagcgcc tgctgatcga gtcgggcggc gaatcgctgc
     2041 gtgccggcgt gcgcaacatg atggaagacc tgacacgcgg caagatctcg cagaccgacg
     2101 agagcgcgtt tgaggtcggc cgcaatgtcg cggtgaccga aggcgccgtg gtcttcgaga
     2161 acgagtactt ccagctgttg cagtacaagc cgctgaccga caaggtgcac gcgcgcccgc
     2221 tgctgatggt gccgccgtgc atcaacaagt actacatcct ggacctgcag ccggagagct
     2281 cgctggtgcg ccatgtggtg gagcagggac atacggtgtt tctggtgtcg tggcgcaatc
     2341 cggacgccag catggccggc agcacctggg acgactacat cgagcacgcg gccatccgcg
     2401 ccatcgaagt cgcgcgcgac atcagcggcc aggacaagat caacgtgctc ggcttctgcg
     2461 tgggcggcac cattgtctcg accgcgctgg cggtgctggc cgcgcgcggc gagcacccgg
     2521 ccgccagcgt cacgctgctg accacgctgc tggactttgc cgacacgggc atcctcgacg
     2581 tctttgtcga cgagggccat gtgcagttgc gcgaggccac gctgggcggc ggcgccggcg
     2641 cgccgtgcgc gctgctgcgc ggccttgagc tggccaatac cttctcgttc ttgcgcccga
     2701 acgacctggt gtggaactac gtggtcgaca actacctgaa gggcaacacg ccggtgccgt
     2761 tcgacctgct gttctggaac ggcgacgcca ccaacctgcc ggggccgtgg tactgctggt
     2821 acctgcgcca cacctacctg cagaacgagc tcaaggtacc gggcaagctg accgtgtgcg
     2881 gcgtgccggt ggacctggcc agcatcgacg tgccgaccta tatctacggc tcgcgcgaag
     2941 accatatcgt gccgtggacc gcggcctatg cctcgaccgc gctgctggcg aacaagctgc
     3001 gcttcgtgct gggtgcgtcg ggccatatcg ccggtgtgat caacccgccg gccaagaaca
     3061 agcgcagcca ctggactaac gatgcgctgc cggagtcgcc gcagcaatgg ctggccggcg
     3121 ccatcgagca tcacggcagc tggtggccgg actggaccgc atggctggcc gggcaggccg
     3181 gcgcgaaacg cgccgcgccc gccaactatg gcaatgcgcg ctatcgcgca atcgaacccg
     3241 cgcctgggcg atacgtcaaa gccaaggcat gacgcttgca tgagtgccgg cgtgcgtcat
     3301 gcacggcgcc ggcaggcctg caggttccct cccgtttcca ttgaaaggac tacacaatga
     3361 ctgacgttgt catcgtatcc gccgcccgca ccgcggtcgg caagtttggc ggctcgctgg
     3421 ccaagatccc ggcaccggaa ctgggtgccg tggtcatcaa ggccgcgctg gagcgcgccg
     3481 gcgtcaagcc ggagcaggtg agcgaagtca tcatgggcca ggtgctgacc gccggttcgg
     3541 gccagaaccc cgcacgccag gccgcgatca aggccggcct gccggcgatg gtgccggcca
     3601 tgaccatcaa caaggtgtgc ggctcgggcc tgaaggccgt gatgctggcc gccaacgcga
     3661 tcatggcggg cgacgccgag atcgtggtgg ccggcggcca ggaaaacatg agcgccgccc
     3721 cgcacgtgct gccgggctcg cgcgatggtt tccgcatggg cgatgccaag ctggtcgaca
     3781 ccatgatcgt cgacggcctg tgggacgtgt acaaccagta ccacatgggc atcaccgccg
     3841 agaacgtggc caaggaatac ggcatcacac gcgaggcgca ggatgagttc gccgtcggct
     3901 cgcagaacaa ggccgaagcc gcgcagaagg ccggcaagtt tgacgaagag atcgtcccgg
     3961 tgctgatccc gcagcgcaag ggcgacccgg tggccttcaa gaccgacgag ttcgtgcgcc
     4021 agggcgccac gctggacagc atgtccggcc tcaagcccgc cttcgacaag gccggcacgg
     4081 tgaccgcggc caacgcctcg ggcctgaacg acggcgccgc cgcggtggtg gtgatgtcgg
     4141 cggccaaggc caaggaactg ggcctgaccc cgctggccac gatcaagagc tatgccaacg
     4201 ccggtgtcga tcccaaggtg atgggcatgg gcccggtgcc ggcctccaag cgcgccctgt
     4261 cgcgcgccga gtggaccccg caagacctgg acctgatgga gatcaacgag gcctttgccg
     4321 cgcaggcgct ggcggtgcac cagcagatgg gctgggacac ctccaaggtc aatgtgaacg
     4381 gcggcgccat cgccatcggc cacccgatcg gcgcgtcggg ctgccgtatc ctggtgacgc
     4441 tgctgcacga gatgaagcgc cgtgacgcga agaagggcct ggcctcgctg tgcatcggcg
     4501 gcggcatggg cgtggcgctg gcagtcgagc gcaaataagg aaggggtttt ccggggccgc
     4561 gcgcggttgg cgcggacccg gcgacgataa cgaagccaat caaggagtgg acatgactca
     4621 gcgcattgcg tatgtgaccg gcggcatggg tggtatcgga accgccattt gccagcggct
     4681 ggccaaggat ggctttcgtg tggtggccgg ttgcggcccc aactcgccgc gccgcgaaaa
     4741 gtggctggag cagcagaagg ccctgggctt cgatttcatt gcctcggaag gcaatgtggc
     4801 tgactgggac tcgaccaaga ccgcattcga caaggtcaag tccgaggtcg gcgaggttga
     4861 tgtgctgatc aacaacgccg gtatcacccg cgacgtggtg ttccgcaaga tgacccgcgc
     4921 cgactgggat gcggtgatcg acaccaacct gacctcgctg ttcaacgtca ccaagcaggt
     4981 gatcgacggc atggccgacc gtggctgggg ccgcatcgtc aacatctcgt cggtgaacgg
     5041 gcagaagggc cagttcggcc agaccaacta ctccaccgcc aaggccggcc tgcatggctt
     5101 caccatggca ctggcgcagg aagtggcgac caagggcgtg accgtcaaca cggtctctcc
     5161 gggctatatc gccaccgaca tggtcaaggc gatccgccag gacgtgctcg acaagatcgt
     5221 cgcgacgatc ccggtcaagc gcctgggcct gccggaagag atcgcctcga tctgcgcctg
     5281 gttgtcgtcg gaggagtccg gtttctcgac cggcgccgac ttctcgctca acggcggcct
     5341 gcatatgggc TgaTGGGCTG AAGGGTTTaa Gcgaagacga acaataaggc ctcccaaatc
     5401 ggggggcctt ttttattgat aacaaaaagg caacactatg acatcggaaa acccgttact
     5461 ggcgctgcga gagaaaatca gcgcgctgga tgaaaaatta ttagcattac tggcagagcg
     5521 gcgcgaaTtC AAACAGAAAG GCCATggctg gcctcaggca tttgagaagc acacggtcac
     5581 actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg
     5641 accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc
     5701 ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa
     5761 aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     5821 cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct
     5881 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     5941 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     6001 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     6061 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     6121 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     6181 gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa
     6241 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     6301 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     6361 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     6421 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     6481 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc
     6541 gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt atttattctg
     6601 cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca
     6661 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
     6721 agctgtccct
//