LOCUS       Exported               13233 bp ds-DNA     circular SYN 25-MAR-2020
DEFINITION  Gateway cloning compatible binary vector for expression of gene by 
            CaMV35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pGWB402.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13233)
  AUTHORS   Nakagawa, T, Suzuki, T, Murata, S, Nakamura, S, Hino, T, Maeo, K, 
            Tabata, R, Kawai, T, Tanaka, K, Niwa, Y, Watanabe, Y, Nakamura, K, 
            Kimura, T, Ishiguro, S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 13233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 27, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13233
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    301..306
                     /label=HindIII
                     /note="Geneious type: motif"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower
                     mosaic virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    1166..1171
                     /label=XbaI
                     /note="Geneious type: motif"
     misc_feature    1172..4457
                     /label=GTTATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATG
                     /label=GTTATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATA 
                     TAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA 
                     AACACAACATATCCAGTCACTATGGCGGCCCCTCGAGGGGCCGCATTAGGCACCCCAG 
                     GCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGC 
                     GAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACC 
                     GTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTC 
                     AATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAA 
                     GAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAAT 
                     GCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTG 
                     TTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAG 
                     TGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGT 
                     TACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCT 
                     CAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAA 
                     CTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTG 
                     ATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAA 
                     TGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGG 
                     ATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGT 
                     ATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAA 
                     GCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATG 
                     ATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCG 
                     TGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATT 
                     GAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGTT 
                     TACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTA 
                     TTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGA 
                     TAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATG 
                     ATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATC 
                     TCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATA 
                     AATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATG 
                     TTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTG 
                     ATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGATAACA 
                     GCGCTT
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1172..1204
                     /label=SV40 large T antigen NLS
     misc_feature    1205..1210
                     /label=SpeI
     misc_feature    1211..1336
                     /label=attR1
                     /note="Geneious type: motif"
     misc_feature    1456..2113
                     /label=CmR
                     /note="Geneious type: motif"
     misc_feature    2457..2759
                     /label=ccdB
                     /note="Geneious type: motif"
     misc_feature    complement(2804..2927)
                     /label=attR2
                     /note="Geneious type: motif"
     misc_feature    2928..2933
                     /label=ApaI
                     /note="Geneious type: motif"
     misc_signal     2935..3630
                     /label=mScarlet-I
     misc_feature    3631..3636
                     /label=MluI
                     /note="Geneious type: motif"
     misc_feature    3637..3726
                     /label=F2A
                     
     misc_feature    3727..3732
                     /label=KpnI
                     /note="Geneious type: motif"
     CDS             3733..4455
                     /label=VENUS
     misc_feature    4459..4464
                     /label=SacI
                     /note="Geneious type: motif"
     terminator      4475..4727
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4761..4777)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4761..4777)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4774..4796)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4785..4801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4809..4839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4854..4875
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      4909..5161
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5551..6345)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5662..5681)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6272..6291
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6366..6549)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(6402..6581)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6755..6779
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7300..8091
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8337..8925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8826..8845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9079..9096
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9111..9251
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9177..9196)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     9337..9359
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9595..9789
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9855..10928)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11357..11986)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgccaaaa aagaagagaa aggtagaaga
     1201 ccccactagt acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     1261 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     1321 tatccagtca ctatggcggc ccctcgaggg gccgcattag gcaccccagg ctttacactt
     1381 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccggcgag attttcagga
     1441 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     1501 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     1561 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     1621 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     1681 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     1741 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     1801 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     1861 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     1921 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     1981 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc
     2041 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     2101 cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca gtatgcgtat
     2161 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     2221 aaaagaggtg tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     2281 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     2341 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     2401 cgcccggttt attgaaatga acggctcttt tgctgacgag aacagggact ggtgaaatgc
     2461 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     2521 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     2581 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     2641 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     2701 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     2761 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     2821 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     2881 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtggg cccaatggtg
     2941 tccaagggcg aagctgtgat taaagaattt atgcgtttta aggtccacat ggaaggatcc
     3001 atgaatggac atgaattcga aatcgagggg gagggcgagg gccgacctta tgagggtaca
     3061 cagaccgcga agctaaaggt aaccaaggga ggcccactac cctttagttg ggacatactc
     3121 agcccgcagt ttatgtatgg cagtcgtgcc tttataaagc acccagcgga tataccagac
     3181 tactacaaac agtccttccc agaggggttt aaatgggagc gagtgatgaa ctttgaggac
     3241 ggtggtgctg tgacggtaac tcaggacaca tcactcgaag acgggactct tatttacaaa
     3301 gtaaaactga gagggaccaa ttttccacca gatggccccg tcatgcaaaa aaagacgatg
     3361 ggttgggaag catctacaga acgactctat cccgaggacg gcgtcctcaa aggcgacatt
     3421 aaaatggcct tgaggttgaa agacggtgga cgttacttgg cggacttcaa aacaacttat
     3481 aaagcaaaaa agccagtgca aatgcctgga gcgtacaatg tagatagaaa acttgatata
     3541 acgtcacata atgaggacta taccgtggtt gagcaatacg agaggtcaga aggacgacac
     3601 agcaccggcg gtatggacga gttgtacaag acgcgtcata agcagaaaat cgttgcacca
     3661 gttaagcaga cgttgaactt tgatttgctt aaactagcag gtgatgttga atccaatcct
     3721 ggacctggta ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3781 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3841 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
     3901 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
     3961 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4021 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4081 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4141 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     4201 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     4261 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4321 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     4381 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4441 gcagctagat ccacctaaga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag
     4501 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     4561 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     4621 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     4681 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattggttcc
     4741 ggaaccaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     4801 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     4861 agctaactca cattaggctg aattaggcgc gcctatttct gaattcccga tctagtaaca
     4921 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
     4981 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
     5041 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca
     5101 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
     5161 cggggatcat ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatat
     5221 cgcggtgcat ctcggtcttg cctgggcagt cgccgccgac gccgttgatg tggacgccgg
     5281 gcccgatcat attgtcgctc aggatcgtgg cgttgtgctt gtcggccgtt gctgtcgtaa
     5341 tgatatcggc accttcgacc gcctgttccg cagagatccc gtgggcgaag aactccagca
     5401 tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca
     5461 acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt
     5521 ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
     5581 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     5641 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     5701 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     5761 attcggcaag caggcatcgc catgggtcac gacgagatca tcgccgtcgg gcatgcgcgc
     5821 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     5881 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     5941 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     6001 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     6061 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     6121 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc
     6181 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     6241 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     6301 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatccaga
     6361 tccggtgcag attatttgga ttgagagtga atatgagact ctaattggat accgagggga
     6421 atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct
     6481 taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc
     6541 cagaaacccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa
     6601 acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat
     6661 gatgaattca gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc
     6721 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     6781 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     6841 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     6901 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     6961 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt
     7021 cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     7081 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     7141 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     7201 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     7261 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc
     7321 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     7381 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     7441 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     7501 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     7561 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     7621 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     7681 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     7741 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     7801 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     7861 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     7921 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     7981 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     8041 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct
     8101 agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     8161 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     8221 cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa
     8281 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8341 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8401 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8461 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     8521 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8581 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8641 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8701 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8761 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8821 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8881 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8941 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     9001 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     9061 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     9121 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     9181 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     9241 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     9301 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     9361 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     9421 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     9481 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     9541 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     9601 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     9661 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     9721 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     9781 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     9841 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     9901 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     9961 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
    10021 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
    10081 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
    10141 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
    10201 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
    10261 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
    10321 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
    10381 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
    10441 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
    10501 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
    10561 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
    10621 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
    10681 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
    10741 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
    10801 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
    10861 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
    10921 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
    10981 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
    11041 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
    11101 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
    11161 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
    11221 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
    11281 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
    11341 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
    11401 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
    11461 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
    11521 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
    11581 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
    11641 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
    11701 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    11761 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    11821 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    11881 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    11941 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    12001 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    12061 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    12121 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    12181 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    12241 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    12301 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    12361 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    12421 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    12481 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    12541 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    12601 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    12661 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    12721 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    12781 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    12841 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    12901 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    12961 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    13021 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    13081 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    13141 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    13201 catgcacata caaatggacg aacggataaa cct
//
