LOCUS       Exported                5149 bp ds-DNA     circular SYN 12-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2020 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             92..1168
                     /codon_start=1
                     /label=Grx1-roCherry
                     /translation="MAQEFVNCKIQPGKVVVFIKPTCPYCRRAQEILSQLPIKQGLLEF
                     VDITATNHTNEIQDYLQQLTGARTVPRVFIGKDCIGGCSDLVSLQQSGELLTRLKQIGA
                     LQTSGTGGNASDGGGSGGVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRP
                     YEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWECTESERMYPED
                     GALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNICLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK"
     promoter        complement(1175..1193)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1198..1332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1445..1463)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1484..1500)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1508..1524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1532..1562)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1577..1598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1886..2474)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2645..3505)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3506..3610)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3636..4091)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4165..5149
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tatggctcaa gagtttgtga actgcaaaat
      121 ccagcctggg aaggtggttg tgttcatcaa gcccacctgc ccgtactgca ggagggccca
      181 agagatcctc agtcaattgc ccatcaaaca agggcttctg gaatttgtcg atatcacagc
      241 caccaaccac actaacgaga ttcaagatta tttgcaacag ctcacgggag caagaacggt
      301 gcctcgagtc tttattggta aagattgtat aggcggatgc agtgatctag tctctttgca
      361 acagagtggg gaactgctga cgcggctaaa gcagattgga gctctgcaga cttccggaac
      421 tgggggcaac gcctctgacg gtggtgggtc tggtggtgtg agcaagggcg aggaggataa
      481 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      541 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      601 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      661 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      721 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      781 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      841 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      901 gtgcaccgaa tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
      961 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     1021 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atctgcttgg acatcacctc
     1081 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     1141 cggcggcatg gacgagctgt acaagtaatc tagaactata gtgagtcgta ttacgtagat
     1201 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     1261 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     1321 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     1381 tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta cccagctttt
     1441 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     1501 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1561 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1621 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1681 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1741 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1801 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1861 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1921 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1981 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2041 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2101 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2161 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2221 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2281 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2341 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2401 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2461 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2521 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2581 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2641 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2701 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2761 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2821 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2881 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2941 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3001 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3061 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3121 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3181 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3241 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3301 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3361 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3421 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3481 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3541 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3601 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     3661 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     3721 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     3781 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     3841 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     3901 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     3961 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     4021 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     4081 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     4141 ggcctcttcg ctattacgcc agtcgaccat agccaattca atatggcgta tatggactca
     4201 tgccaattca atatggtgga tctggacctg tgccaattca atatggcgta tatggactcg
     4261 tgccaattca atatggtgga tctggacccc agccaattca atatggcgga cttggcacca
     4321 tgccaattca atatggcgga cttggcactg tgccaactgg ggaggggtct acttggcacg
     4381 gtgccaagtt tgaggagggg tcttggccct gtgccaagtc cgccatattg aattggcatg
     4441 gtgccaataa tggcggccat attggctata tgccaggatc aatatatagg caatatccaa
     4501 tatggcccta tgccaatatg gctattggcc aggttcaata ctatgtattg gccctatgcc
     4561 atatagtatt ccatatatgg gttttcctat tgacgtagat agcccctccc aatgggcggt
     4621 cccatatacc atatatgggg cttcctaata ccgcccatag ccactccccc attgacgtca
     4681 atggtctcta tatatggtct ttcctattga cgtcatatgg gcggtcctat tgacgtatat
     4741 ggcgcctccc ccattgacgt caattacggt aaatggcccg cctggctcaa tgcccattga
     4801 cgtcaatagg accacccacc attgacgtca atgggatggc tcattgccca ttcatatccg
     4861 ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc cacttggcag tacatcaata
     4921 tctattaata gtaacttggc aagtacatta ctattggaag gacgccaggg tacattggca
     4981 gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat ggctgccaag tacatcccca
     5041 ttgacgtcaa tggggagggg caatgacgca aatgggcgtt ccattgacgt aaatgggcgg
     5101 taggcgtgcc taatgggagg tctatataag caatgctcgt ttagggaac
//
