LOCUS       Exported                4814 bp ds-DNA     circular SYN 13-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 13, 2020 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        10..24
                     /label=T5 promoter
     promoter        25..30
                     /label=-35
                     /label=T5 promoter
     protein_bind    30..46
                     /label=lac operator
     promoter        31..47
                     /label=T5 promoter
     promoter        48..54
                     /label=-10
                     /label=T5 promoter
     protein_bind    62..78
                     /label=lac operator
     RBS             97..108
                     /label=RBS
     CDS             127..144
                     /label=6xHis
     misc_feature    151..1539
                     /label=RexYFP
     misc_feature    388..396
                     /label=linker 1
     misc_feature    397..1134
                     /label=cpYFP
     misc_feature    1135..1140
                     /label=linker 2
     terminator      1561..1655
                     /label=lambda t0 terminator
     CDS             1699..2358
                     /label=CmR
     terminator      2423..2509
                     /label=rrnB T1 terminator
     misc_feature    2664..2804
                     /label=bom
     rep_origin      complement(2990..3578)
                     /direction=LEFT
                     /label=ori
     CDS             complement(3749..4540)
                     /label=AmpR
     CDS             complement(4541..4609)
                     /label=signal sequence
                     /label=AmpR
     promoter        complement(4610..4714)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc atgaaggtcc ccgaagcggc catcagccgc
      181 ctgatcacct acctccgcat cctggaggag ctggaggccc agggggttca ccggacgagc
      241 tccgagcagc ttggggagct cgcccaggtc acggccttcc aggtgcggaa ggacctttcc
      301 tacttcggct cctacgggac gcgcggggtg gggtacaccg tccccgtcct gaagcgggag
      361 ctccgccaca tcctggggct caaccgctcc gccggctaca acagcgacaa cgtctatatc
      421 atggccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacgtcgag
      481 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      541 gtgctgctgc ccgacaacca ctacctgagc ttccagtccg tcctgagcaa agaccccaac
      601 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactcccggc
      661 atggacgagc tgaacaacgt ggatggcggt agcggtggca ccggcagcaa gggcgaggag
      721 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      781 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
      841 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctcggctac
      901 ggcctgaagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      961 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1021 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1081 ggcatcggct tcaaggagga cggcaacatc ctggggcaca agctggagta caacggcacc
     1141 aagtggggcc tgtgcatcgt gggcatgggc cggctcggga gcgccctggc cgactacccg
     1201 gggttcgggg agagctttga gctcaggggc ttctttgacg tggacccgga gaaggtgggc
     1261 cgcccggtgc ggggcggggt cattgagcac gtggacctcc tgccccagcg ggttcccggc
     1321 cgcattgaga tcgccctcct caccgtgccc cgggaagcgg cccagaaggc ggcggacctg
     1381 ttggtggcgg cggggatcaa ggggatcctg aacttcgccc cggtggtcct cgaggtgccc
     1441 aaggaggtgg cggtggagaa cgtggacttc ttggcggggc tcacgcggct ttctttcgct
     1501 atcctgaacc ccaagtggcg ggaagagatg atggggtgaa agcttaatta gctgagcttg
     1561 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     1621 tcggttgccg ccgggcgttt tttattggtg agaatccaag ctagcttggc gagattttca
     1681 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     1741 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     1801 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     1861 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt
     1921 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     1981 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     2041 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     2101 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     2161 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     2221 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     2281 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     2341 tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggggta
     2401 atgactctct agcttgaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     2461 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccctctag
     2521 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     2581 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     2641 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     2701 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     2761 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     2821 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2881 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2941 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3001 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3061 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3121 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3181 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3241 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3301 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3361 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3421 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3481 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3541 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3601 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3661 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3721 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     3781 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     3841 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3901 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3961 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4021 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4081 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4141 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4201 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4261 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4321 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4381 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4441 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4501 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4561 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4621 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4681 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     4741 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     4801 cctttcgtct tcac
//
