LOCUS       Exported                3407 bp ds-DNA     circular SYN 05-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 16, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
COMMENT     LOCUS pYPQ104_correct_ 3623 bp ds-DNA circular 02-MAR-2014 SOURCE 
            ORGANISM COMMENT COMMENT ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..13
                     /label=For Left homology arm
     promoter        21..397
                     /label=OsU3 promoter
                     /note="OsU3 promoter"
     promoter        21..43
                     /label=AAGGGATCTTTAAACATACGAAC
                     /note="pYPQ-13xD-F"
     primer_bind     21..43
                     /label=Primer 1
     misc_structure  399..430
                     /label=guide RNA cloning site
                     /note="guide RNA cloning site"
     misc_feature    431..506
                     /label=gRNA scaffold
                     /note="gRNA scaffold"
     misc_feature    507..536
                     /label=RT template + Primer binding
     misc_feature    508..513
                     /label=BsaI site
                     /note="BsaI site"
     misc_feature    530..535
                     /label=BsaI site
                     /note="BsaI site"
     terminator      537..543
                     /note="terminator"
     terminator      544..598
                     /label=OsU6-2 terminator
                     /note="OsU6-2 terminator"
     misc_feature    complement(576..598)
                     /label=AAGCTTATCGATACCCTCGACCT
                     /note="pYPQ13xD-R"
     primer_bind     complement(576..598)
                     /label=Primer 2
     misc_feature    606..705
                     /label=attL2
     primer_bind     complement(722..743)
                     /label=T7
     primer_bind     complement(742..762)
                     /label=M13-rev
     misc_feature    complement(746..762)
                     /label=M13-R
     misc_feature    840..973
                     /label=Spn promoter
     misc_feature    974..1984
                     /label=SpnR
     misc_feature    2051..2724
                     /label=pUC origin
     misc_feature    3247..3270
                     /label=M13-F1
                     /note="M13-F1"
     primer_bind     3263..3280
                     /label=M13-fwd
     misc_feature    3264..3279
                     /label=M13-F
     misc_feature    3282..3394
                     /label=attL5
ORIGIN
        1 ctagataacg caggatccta aagggatctt taaacatacg aacagatcac ttaaagttct
       61 tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag atgtgcagtc
      121 agggaccata gcacaggaca ggcgtcttct actggtgcta ccagcaaatg ctggaagccg
      181 ggaacactgg gtacgttgga aaccacgtga tgtggagtaa gataaactgt aggagaaaag
      241 catttcgtag tgggccatga agcctttcag gacatgtatt gcagtatggg ccggcccatt
      301 acgcaattgg acgacaacaa agactagtat tagtaccacc tcggctatcc acatagatca
      361 aagctggttt aaaagagttg tgcagatgat ccgtggcaga gacgagatct agtctgagtc
      421 gaccgtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
      481 ttgaaaaagt ggcaccgagt cggtgcggag accgacgtca attgctagcg gtctcatttt
      541 tttctgcaga tatccatcac actggcggcc gctcgaggtc gagggtatcg ataagcttga
      601 attcgaccca gctttcttgt acaaagttgg cattataaaa aataattgct catcaatttg
      661 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
      721 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
      781 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctggac
      841 cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc
      901 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
      961 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     1021 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     1081 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     1141 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga
     1201 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     1261 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     1321 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     1381 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     1441 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     1501 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     1561 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     1621 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     1681 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     1741 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     1801 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     1861 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     1921 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     1981 ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     2041 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2101 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2161 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2221 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2281 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2341 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2401 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2461 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2521 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2581 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2641 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2701 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2761 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2821 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2881 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2941 gcgcaacgca attaatacgc gtaccgctcg ccaggaagag tttgtagaaa cgcaaaaagg
     3001 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     3061 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
     3121 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
     3181 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgcttgcatg
     3241 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggcccaaata
     3301 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
     3361 tttataatgc caactttgta tacaaaagtt gccccatggc gttccct
//
