LOCUS       psL1180                 8270 bp ss-DNA     circular SYN 09-Mar-2020
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
SUMMARY     Length:           6906 bp (0 gaps)
            Reads:            3
            Avg Read Length:  972 bp (963 bp after trimming)
            Fold Coverage:    0.4 (Top strand 0.1, Bottom strand 0.3)
FEATURES             Location/Qualifiers
     source          <5580..5584
                     /note=" [Split]"
     CDS             complement(942..1802)
                     /note="Amp"
     regulatory      complement(1844..1872)
                     /note="AmpR_promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(2031..2053)
                     /note="pGEX_3_primer"
     rep_origin      complement(2321..2627)
                     /note="f1_origin"
     CDS             complement(2728..2871)
                     /note="lacZ_a"
     primer_bind     2842..2864
                     /note="M13_pUC_fwd_primer"
     primer_bind     2857..2873
                     /note="M13_forward20_primer"
     misc_feature    2971..2985
                     /note="15 bp left arm Nsil FWD"
     misc_feature    2986..3996
                     /note="Left arm"
     primer_bind     2986..3015
                     /note="DT_LeftBrp_Nsil_F"
     CDS             3785..3855
                     /note="Brp_3rdLastExon"
     primer_bind     3787..3804
                     /note="ZZ_MiSeq_brp3_R"
     CDS             3911..>3996
                     /codon_start=2
                     /note="Brp_2ndLastExon [Split]"
     misc_feature    3933..3957
                     /note="REV primer"
     primer_bind     complement(3968..3996)
                     /note="DT_LeftBrp_Nsil_R"
     primer_bind     3977..>3996
                     /note="ZZ_MiSeq_brp1_R [Split]"
     misc_RNA        3980..>3996
                     /note="sgRNA-brp3 [Split]"
     misc_feature    3996
                     /note="brp3-cutting [Split]"
     misc_feature    3997..4132
                     /note="Brp changed codons"
     misc_feature    4133..4147
                     /note="15 bp left arm Nsil REV"
     CDS             4134..4142
                     /note="GSG"
     CDS             4143..4196
                     /note="Plos-T2A"
     CDS             4197..5258
                     /note="QF2w"
     primer_bind     4197..4217
                     /note="ZZ_QF2_F"
     primer_bind     complement(4201..4224)
                     /note="Brp_T2A_R"
     misc_feature    4210..4224
                     /note="15 bp overhang"
     primer_bind     4210..4229
                     /note="DT_QF2w_Hsp70T_F"
     primer_bind     complement(4351..4370)
                     /note="ZZ_verify_QF2_R"
     primer_bind     complement(5493..5534)
                     /note="DT_QF2w_Hsp70T_R"
     misc_feature    <5520..5528
                     /note="LoxP [Split]"
     primer_bind     5520..5547
                     /note="Brp_T2A_QF2_F"
     misc_feature    5520..5534
                     /note="15bp overhang"
     misc_recomb     5530..5579
                     /note="attP"
     misc_feature    <5580..5584
                     /note=" [Split]"
     misc_feature    5585..5597
                     /note="Stuffer extension"
     misc_feature    5595..>5597
                     /note="Asc1 [Split]"
     CDS             5873..6553
                     /note="dsRED"
     primer_bind     complement(5892..5912)
                     /note="ZZ_sanger_dsRed2"
     primer_bind     6221..6239
                     /note="ZZ_sanger_dsRed1"
     primer_bind     complement(6362..6379)
                     /note="ZZ_sanger_dsRed3"
     primer_bind     6784..>6795
                     /note="SEM_Fu_3R_gsg_3R_psl1180_for [Split]"
     misc_feature    6787..6801
                     /note="15 bp overlap right arm FWD"
     misc_feature    join(6798..>6801,<7845..7854)
                     /note="15 bp overlap right arm REV [Split]"
     primer_bind     6802..6832
                     /note="DT_RightBrp_AvrII_F"
     misc_feature    6802
                     /note="right arm"
     misc_feature    6802..7844
                     /note="Right arm"
     misc_feature    <6802
                     /note=" [Split]"
     misc_RNA        <6802..6804
                     /note=" [Split]"
     primer_bind     <6802..6803
                     /note=" [Split]"
     CDS             <6802..6850
                     /codon_start=3
                     /note="Brp_2ndLastExon [Split]"
     primer_bind     6860..6881
                     /note="ZZ_MiSeq_brp3_F"
     CDS             6921..7011
                     /codon_start=2
                     /note="Brp_lastExon"
     misc_RNA        6970..6989
                     /note="sgRNA-brp2"
     misc_feature    6972..6973
                     /note="brp-cutting"
     misc_RNA        6985..7004
                     /note="sgNA-brp1"
     primer_bind     complement(7041..7061)
                     /note="ZZ_MiSeq_brp1_F"
     primer_bind     complement(7811..7844)
                     /note="DT_RightBrp_AvrII_R"
     primer_bind     complement(8047..8065)
                     /note="M13_reverse_primer"
     primer_bind     complement(8064..8086)
                     /note="M13_pUC_rev_primer"
     regulatory      complement(8100..8129)
                     /note="lac_promoter"
                     /regulatory_class="promoter"
BASE COUNT      2235 A      2054 C      1928 G      2053 T         0 OTHER
ORIGIN      ?
        1 TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA
       61 TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
      121 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG
      181 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG
      241 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
      301 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA
      361 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
      421 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT
      481 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
      541 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
      601 CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT
      661 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
      721 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT
      781 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
      841 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
      901 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
      961 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     1021 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     1081 CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     1141 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     1201 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     1261 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     1321 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     1381 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     1441 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     1501 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     1561 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     1621 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     1681 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     1741 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     1801 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     1861 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
     1921 AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG
     1981 CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC
     2041 ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC
     2101 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA
     2161 GAGCAGATTG TACTGAGAGT GCACCATAAA ATTGTAAACG TTAATATTTT GTTAAAATTC
     2221 GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC
     2281 CCTTATAAAT CAAAAGAATA GCCCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG
     2341 AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC
     2401 GATGGCCCAC TACGTGAACC ATCACCCAAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA
     2461 GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG
     2521 AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT
     2581 GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC
     2641 GCGTACTATG GTTGCTTTGA CGTATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA
     2701 AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG
     2761 TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA
     2821 GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGCCAAGC
     2881 TTAAGGTGCA CGGCCCACGT GGCCACTAGT ACTTCTCGAG CTCTGTACAT GTCCGCGGTC
     2941 GCGACGTACG CGTATCGATG GCGCCAGCTG CAGGCGGCCG CCATAATGAC CGGCTACCAT
     3001 GACCACTTTA TAGTAGGTTC CAGGTGCCCC GGGGGATGTG GACAATTCGG AACAGTGGTT
     3061 GCCCAGTGGT CAATATGCAT GGCCAAGGAA GTTCCTAAAA CTGGAACAAA TTTGCCGTCG
     3121 ACTGCTTCCA GATGCATCCA ATTTCATCCA TTTTTCATAA AGTTATAAGC ATTTGAATTT
     3181 TGGCAAAATT TTGCCTATCT CAATCGTTTT GCCTATCCCC TGTAGGATTC TGTTTGAATT
     3241 CTGACCAGGA TTCATTCTGG ATTGAACAGG TTTCTAGACA GGACTATGAA ACAAACCGTA
     3301 GGCATGATTT CTTAGAACAA AATCCTTTAT GGATTGATAG ATTAATAATT GAATTATCTT
     3361 TACTGAAAAT ACTTTCAACC GTCAGGTGGT TCGTCTTCAA TCTCCAATGT GGATTTCTGA
     3421 CCGAATTTTG AGAGGTTTTC TTCTCATCTG CATGCCAGTG CAAAATAGTT TTAAAAACTA
     3481 GACTAGATAA CACAACCCTG AGCACGGTTC TCACTGAATA CTCTGTACAA TGTTTGATAG
     3541 CAAGTATCTT GACCCAATTT TGGCCCATCA CCAAATACTG CTGCTGAGAT TTGTCTCACG
     3601 ATTTAAATAG TTTGGAGGGC CTGCTTTAGG TTCAAAACTT TCGTCAGCAG AAAGATTTTG
     3661 TGTACTTATT ACGTTGGATT CCTCTCTATG ATAAATCAAA TCATTTCATT TCATTTATAA
     3721 CAATATCCTT TCAACGTTTA GAATAACCGC TTCTAATATC TACAAATGTT TGATTTTTTT
     3781 GCAGGCTGGA GGTACAGCAG CAGCAGCCGG AGAATTGAAC AAACAGTTAA CAGAAACTCA
     3841 ACAACAATTG GACAAGTAAG TAAACCAAAA AGTCAGCAAA AACACTTCTT CAATGTTTCA
     3901 TAATCCACAG GATTACCAAA GAATTGAACG ACTCAAAAGA AGAGGCTCAG CGATCAGCAG
     3961 CCGAAACGGA ACGTTTACTG CAACTGGTAC AGATGACCCA GGAAGAACAG AACCAGAAGG
     4021 AAAAGACCAT CATGGATCTG CAGCAGGCCC TGAAGAACGC CCAGGCCAAG CTGAAGACCG
     4081 CCCAGTCGCA GCCGCAGGAT GCCGGACCGG CCGGATTCCT GAAGTCGTTC TTTGGATCGG
     4141 GAGAGGGCCG CGGCTCCCTG CTGACCTGCG GCGACGTGGA GGAGAACCCC GGCCCCATGC
     4201 CACCCAAGCG CAAAACGCTT AACGCTGCGG CTGAGGCTAA CGCTCATGCC GACGGACACG
     4261 CCGACGGAAA CGCCGACGGA CACGTGGCCA ATACGGCCGC GTCCTCGAAT AATGCGAGGT
     4321 TCGCTGATCT CACTAACATC GATACTCCGG GTCTGGGACC CACAACTACG ACCCTGCTCG
     4381 TGGAACCAGC ACGCTCAAAG CGTCAACGAG TGTCCCGCGC ATGCGACCAG TGCCGTGCAG
     4441 CCCGAGAGAA ATGCGACGGA ATACAGCCTG CGTGTTTCCC GTGCGTTTCC CAGGGAAGGT
     4501 CCTGCACTTA TCAGGCTTCG CCGAAAAAGA GGGGAGTTCA AACCGGTTAT ATTCGTACGC
     4561 TGGAGCTCGC CCTCGCCTGG ATGTTTGAAA ATGTCGCGCG TTCCGAAGAT GCCTTGCATA
     4621 ACCTCCTCGT CCGTGACGCC GGACAAGGAT CAGCTCTGCT CGTTGGTAAA GATTCGCCGG
     4681 CTGCCGAGCG ACTCCATGCC CGTTGGGCTA CTAGCCGTGT CAATAAGAGC ATTACCCGCC
     4741 TCCTCCGTCA GTTGGAGCTC CCTCCTACCG CCACGGCTAC GGCCTCGATA ATGCCGCACG
     4801 TGATGGAGCA GCCTCTCAGT ACCAGCATTA ACCCCGTCAA CGACCGCTTC AACGGTATTC
     4861 CCAACCCCAC TCCGTATAAC TCCGATGCAG CTCTCGATGC TATCACTCAG ACCAACGATT
     4921 ATGGAAGCGT AAATACACAT GGTATCCTCT CTACTTACCC GCCACCGGCT ACGCACCTTA
     4981 ATGAAGCTTC CGTCGCTCTC GCTCCCGGTG GCGCCCCCCC CCGACCGCCT CCTCCGTATG
     5041 TTGACAGCAC GACCAATCAC CCGCCGTACC ACTCGAATCT GGTTCCAATG GCGAACTTTG
     5101 GTTACTCGAC CGTTGATTAC GATGCCATGG TTGACGATTT GGCTAGCATT GAATACACGG
     5161 ACGCTGTGGA TGTCGACCCA CAGTTTATGA CCAATCTGGG ATTCGTTCCT GGATGTAACT
     5221 TCTCCGACAT TAATACATAC AAAAAGAAGA AATGATGACG TCTATCGATA CCGTCGACTA
     5281 AAGCCAAATA GAAAATTATT CAGTTCCTGG CTTAAGTTTT TAAAAGTGAT ATTATTTATT
     5341 TGGTTGTAAC CAACCAAAAG AATGTAAATA ACTAATACAT AATTATGTTA GTTTTAAGTT
     5401 AGCAACAAAT TGATTTTAGC TATATTAGCT ACTTGGTTAA TAAATAGAAT ATATTTATTT
     5461 AAAGATAATT GCGTTTTTAT TGTCAGGGAG TGAGTTTGCT TAAAAACTCG TTTAGATCCC
     5521 GAAGTTATCC TACGCCCCCA ACTGAGAGAA CTCAAAGGTT ACCCCAGTTG GGGCACTACT
     5581 CTAGATACGC GTACGGCAAT TCGAGCTCGC CCGGGGATCT AATTCAATTA GAGACTAATT
     5641 CAATTAGAGC TAATTCAATT AGGATCCAAG CTTATCGATT TCGAACCCTC GACCGCCGGA
     5701 GTATAAATAG AGGCGCTTCG TCTACGGAGC GACAATTCAA TTCAAACAAG CAAAGTGAAC
     5761 ACGTCGCTAA GCGAAAGCTA AGCAAATAAA CAAGCGCAGC TGAACAAGCT AAACAATCGG
     5821 GGTACCGCTA GAGTCGACGG TACCGCGGGC CCGGGATCCA CCGGTCGCCA CCATGGTGCG
     5881 CTCCTCCAAG AACGTCATCA AGGAGTTCAT GCGCTTCAAG GTGCGCATGG AGGGCACCGT
     5941 GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC CGCCCCTACG AGGGCCACAA
     6001 CACCGTGAAG CTGAAGGTGA CCAAGGGCGG CCCCCTGCCC TTCGCCTGGG ACATCCTGTC
     6061 CCCCCAGTTC CAGTACGGCT CCAAGGTGTA CGTGAAGCAC CCCGCCGACA TCCCCGACTA
     6121 CAAGAAGCTG TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG
     6181 CGGCGTGGTG ACCGTGACCC AGGACTCCTC CCTGCAGGAC GGCTGCTTCA TCTACAAGGT
     6241 GAAGTTCATC GGCGTGAACT TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA AGACCATGGG
     6301 CTGGGAGGCC TCCACCGAGC GCCTGTACCC CCGCGACGGC GTGCTGAAGG GCGAGATCCA
     6361 CAAGGCCCTG AAGCTGAAGG ACGGCGGCCA CTACCTGGTG GAGTTCAAGT CCATCTACAT
     6421 GGCCAAGAAG CCCGTGCAGC TGCCCGGCTA CTACTACGTG GACTCCAAGC TGGACATCAC
     6481 CTCCCACAAC GAGGACTACA CCATCGTGGA GCAGTACGAG CGCACCGAGG GCCGCCACCA
     6541 CCTGTTCCTG TAGCGGCCGC GACTCTAGAT CATAATCAGC CATACCACAT TTGTAGAGGT
     6601 TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC
     6661 AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT
     6721 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT
     6781 CATCAATGTA TCTTATCCTA GCACAGGAAG AGCAGAACCA AAAAGAAAAG ACCATTATGG
     6841 ATCTGCAACA GTGAGTTTTC TGGCATTAGT ATTGGCACAG TAAATGCACT ATATTCATAT
     6901 TATTCTTACC TTTTTTGCAG AGCTTTAAAA AACGCTCAAG CAAAGTTGAA GACAGCACAA
     6961 TCACAGCCGC AAGATGCTGG ACCAGCCGGT TTCCTGAAGA GCTTCTTCTA AGCAATACGC
     7021 CGCCGGTATT CCAACAATTT CTTCAAACTG CAGAGCACCA GAGATGGCGT TGTGTAGTTA
     7081 TCCATGTAGC CACTTGTTTA AGATTCCTTG AGTACAGTCC CCAAGTTATT GTGATTATCT
     7141 TGAATTCCTT GCATACTTAA TTACCTATGA ACACGTGTTG TTCCTGTTGA TTCGAATCAG
     7201 AGCAACGGTT ATTTTCTCAC CGTATGAATC GTATAGGGAA CCGATCAACA TCTTGTTCAT
     7261 AAGCAATATA TCATATGTAA AAATTGTTAG GACGTGTCGA TCTAGCGAAA TAATTGTTGA
     7321 AAGATACAAA CTAATACAAA CCATGCTGCC ATATTGATAA AACTAAGTAA AAGGGTAAGA
     7381 TAAACTACGG AAAAAGTGAT ATTTACCTAG TCATTATCGC TACACTCGAA GTACTTTAAC
     7441 CAGCGCATGA GAATTCGAGA TAGTCATTGA ATTATATACT GTTAAATAGA TATTTATTGT
     7501 AAAAAGTATA CACGCGGGTA ACGTGCATAT TTTTTCTATT TTCGCTTAAA TATAAAAAGG
     7561 CCCATTAGAT ATGGGCAGTT TGTTCACGAT TTCAATCATG ATCCACTTAG ATTCGACGAT
     7621 ACGTACTTCA ATTTTATTTT CGTTGAGACA TTTCATTATT TGATTCACCA ATTGAGGTTA
     7681 TCGGTACACT ATTTTGGATA GAATCCACTA CGTCTCCTAA CTAACAATCT AATTTACTAG
     7741 GCATTGTAAG AGTCTGGGTT TGATGGACTA CTCGCCTATC TTGAAGGAGC ACCACTGCTG
     7801 GTTGAACATG AGTTCTTATC GGGAAATTTG TCACAGATTC GAAACCTATT AATATTCCGG
     7861 AGTATACGTA GCCGGCTAAC GTTAACAACC GGTACCTCTA GAACTATAGC TAGCATGCGC
     7921 AAATTTAAAG CGCTGATATC GATCGCGCGC AGATCTGTCA TGATGATCAT TGCAATTGGA
     7981 TCCATATATA GGGCCCGGGT TATAATTACC TCAGGTCGAC GTCCCATGGC CATTCGAATT
     8041 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
     8101 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
     8161 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
     8221 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
//
