LOCUS       RA035                   8280 bp ds-DNA     circular     10-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(3506..3522)
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    4560..4578
                     /label="XylI2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(3127..3145)
                     /label="R0071 pRhl from pseudomonas PAO1"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    complement(3583..4147)
                     /label="Spacer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4579..4584
                     /label="-35"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(3072..3090)
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    4717..5465
                     /label="AiiA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4641..4706
                     /label="BCD"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4537..4557
                     /label="XylI1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5467..5499
                     /label="ssrA"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    91..185
                     /label="B0014terminator"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     misc_feature    4615..4633
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    complement(1942..2988)
                     /label="LacI_min11"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    complement(2999..3064)
                     /label="BCD"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4602..4607
                     /label="-10"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(3104..3120)
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    complement(5960..6032)
                     /label="pSC101"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(3121..3126)
                     /label="-35"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(6036..8175)
                     /label="pSC101"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4162..4178
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    4585..4601
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    6033..6035
                     /label="Extra bp"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(3098..3103)
                     /label="-10"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1664..1758)
                     /label="B0014terminator"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     misc_feature    complement(735..1394)
                     /label="CAM_R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1903..1941)
                     /label="ssrA"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 GAAGTTCCTA TTCtctagaa aGTATAGGAA CTTCaacgcc atgagcggcc tcatttctta
       61 ttctgagtta caacagtccg caccgctgtc tcacactggc tcaccttcgg gtgggccttt
      121 ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg gcttttttat
      181 tatttcctag cggccgcagg cggtagctcc ttccggtggg cgcggggcat gactatcgtc
      241 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgccc
      301 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gccctgcacc
      361 attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct
      421 gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa atgatcatat
      481 cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc
      541 acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg
      601 ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc
      661 attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa
      721 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      781 agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc
      841 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
      901 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
      961 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1021 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1081 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1141 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     1201 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1261 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1321 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1381 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1441 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     1501 tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac accGAAGTTC
     1561 CTATTCtcta gaaaGTATAG GAACTTCtgc tcatgtttga cagcttatca tcgatgcata
     1621 atgtgcctgt caaatggacg aagcagggat tctGCTAGCt aggaaataat aaaaaagccg
     1681 gattaataat ctggcttttt atattctctc tctagtatat aaacgcagaa aggcccaccc
     1741 gaaggtgagc cagtgtgaga cagcggtgcg gactgttgta actcagaata agaaatgagg
     1801 ccgctcatgg cgttgactct cagtcatagt atcgtggtat caccggttgg ttccactctc
     1861 tgttgcgggc aacttcagca gcacgtaggg gAAGCTTGGt caCGCTGCAA GGGCGTAATT
     1921 TTCGTCGTTC GCTGCACTAG Tcagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1981 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     2041 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     2101 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     2161 ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat
     2221 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     2281 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     2341 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     2401 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     2461 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     2521 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     2581 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     2641 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     2701 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     2761 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     2821 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     2881 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     2941 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcatac tagtatttct
     3001 ccttagcatg attaagatgt ttcagtacga aaattgcttt cattgttgat ctccttttta
     3061 agtggcatgc atctctatca ctgataggga tGTGCTCAGT ATCtctatca ctgataggga
     3121 AGTAAAactg ccagatttca caggatcgac agttcatagg tgattgctca ggacatttct
     3181 gttagaagga atcgttttcc ttacttttcc ttacgcacaa gagttccgta gctgttcaag
     3241 tttgtgtttc aactgttctc gtcgtttccg caacaagtcc tcttcagaaa tgagcttttg
     3301 ctcctctgct tggacggaca ggatgtatgc tgtggctttt ttaaggataa ctaccttggg
     3361 ggccttttca ttgttttcca actccgggat ctggtcacgc agggcaaaaa agctccgttt
     3421 tagctcgttc ctcctctggc gctccaagac gttgtgtgtt cgcctcttga cattctcctc
     3481 ggtgtccgag ggccctgtgt gaatctctat cactgatagg gatccacaca gacgtcggaa
     3541 ttgccagctg gggcgccctc tggtaaggtt gggaagCTCG AGaagcgcgt ctgaataggc
     3601 ttcttgtatt cctgccacac agggaaacca tcaggagtta cccaatgcac agcgcaacgc
     3661 ttgcgaagaa tctctccagt cttcttatct ttgacctcag cagccagcag cttagcagca
     3721 gacttaagcc agttcattgc ttcaaccgca gctaccaccg tcacgctcac agattcccaa
     3781 atcagcttag ccatgtatcc agcagcctga ttcggctgag tgaacatcag acccttgccg
     3841 gaatcaatag ctggctgaat ggtatcttcc agcacttgtt gacggaagcc gaactctttg
     3901 gacccgtaag ccagcgtcat gactgaacgc ttagtcacac tgcgagtaac accgtaagcc
     3961 agccattgac cagccagtgc cttagtgccc agcttgactt tctcagagat ttcaccagtg
     4021 ttctcatcgg tcacggtaac tacttcgtta tcggtcccat tgattgcgtc tgcttgtaga
     4081 atctcgttga ctttcttagc aacaatcccg tagatgtcct gaacggtttc actaggaagc
     4141 aagttaagac gtctgtgtgg atccctatca gtgatagaga ttcacacagg gccctcggac
     4201 accgaggaga atgtcaagag gcgaacacac aacgtcttgg agcgccagag gaggaacgag
     4261 ctaaaacgga gcttttttgc cctgcgtgac cagatcccgg agttggaaaa caatgaaaag
     4321 gcccccaagg tagttatcct taaaaaagcc acagcataca tcctgtccgt ccaagcagag
     4381 gagcaaaagc tcatttctga agaggacttg ttgcggaaac gacgagaaca gttgaaacac
     4441 aaacttgaac agctacggaa ctcttgtgcg taaggaaaag taaggaaaac gattccttct
     4501 aacagaaatg tcctgagcaa tcacctatga actgtcgaaa taaaccaaaa atcgtaatcg
     4561 aaagataaaa atctgtaaTT TACTtcccta tcagtgatag aGATACTGAG CACatcccta
     4621 tcagtgatag agatgcatgc cacttaaaaa ggagatcaac aatgaaagca attttcgtac
     4681 tgaaacatct taatcatgct aaggagaaat actagtatga cagtaaagaa actttatttc
     4741 atcccagcag gtcgttgcat gttggatcat tcgtctgtta acagtgcgtt aacaccgggg
     4801 aaactattaa acttgccggt gtggtgttat cttttggaga cggaagaagg tcctatttta
     4861 gtagacacag gtatgccaga aagtgcagtt aataatgaag ggctttttaa cggtacattt
     4921 gttgaaggac agatcttacc gaaaatgact gaagaagata gaatcgtgaa tatattaaag
     4981 cgtgtagggt atgagccgga cgacctttta tatattatta gttctcactt acattttgat
     5041 catgcaggag gaaacggtgc ttttacaaat acaccaatta ttgtgcagcg aacggaatat
     5101 gaggcagcac ttcatagaga agaatatatg aaagaatgta tattaccgca tttgaactac
     5161 aaaattattg aaggggatta tgaagtggta ccaggtgttc aattattgta tacgccaggt
     5221 cattctccag gccatcagtc gctattcatt gagacggagc aatccggttc agttttatta
     5281 acgattgatg catcgtacac gaaagagaat tttgaagatg aagtgccgtt cgcaggattt
     5341 gatccagaat tagctttatc ttcaattaaa cgtttaaaag aagttgtgaa aaaagagaaa
     5401 ccaattattt tctttggtca tgatatagag caggaaaaga gttgtagagt gttcccggaa
     5461 tatatagcag cgaacgacga aaattacgcc cttgcagcgt aataaGAGCT CCTAGAGTGC
     5521 GCGACAGGTT TGATGACAAA AAATTAGCGC AAGAAGACAA AAATCACCTT GCGCTAATGC
     5581 TCTGTTACAG GTCACTAATA CCATCTAAGT AGTTGATTCA TAGTGACTGC ATATGTTGTG
     5641 TTTTACAGTA TTATGTAGTC TGTTTTTTAT GCAAAATCTA ATTTAATATA TTGATATTTA
     5701 TATCATTTTA CGTTTCTCGT TCAgcttttt tatactaaGT TGGCATTATA AAAAAGCATT
     5761 GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGAT
     5821 TTCAATTTTG TCCCACTCCC CCTAGAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA
     5881 CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC
     5941 GCCGCCCTAG ACCTAGGGTA CGGGTTTTGC TGCCCGCAAA CGGGCTGTTC TGGTGTTGCT
     6001 AGTTTGTTAT CAGAATCGCA GATCCGGCTT CAGCCGGTTT GCCGGCTGAA AGCGCTATTT
     6061 CTTCCAGAAT TGCCATGATT TTTTCCCCAC GGGAGGCGTC ACTGGCTCCC GTGTTGTCGG
     6121 CAGCTTTGAT TCGATAAGCA GCATCGCCTG TTTCAGGCTG TCTATGTGTG ACTGTTGAGC
     6181 TGTAACAAGT TGTCTCAGGT GTTCAATTTC ATGTTCTAGT TGCTTTGTTT TACTGGTTTC
     6241 ACCTGTTCTA TTAGGTGTTA CATGCTGTTC ATCTGTTACA TTGTCGATCT GTTCATGGTG
     6301 AACAGCTTTA AATGCACCAA AAACTCGTAA AAGCTCTGAT GTATCTATCT TTTTTACACC
     6361 GTTTTCATCT GTGCATATGG ACAGTTTTCC CTTTGATATC TAACGGTGAA CAGTTGTTCT
     6421 ACTTTTGTTT GTTAGTCTTG ATGCTTCACT GATAGATACA AGAGCCATAA GAACCTCAGA
     6481 TCCTTCCGTA TTTAGCCAGT ATGTTCTCTA GTGTGGTTCG TTGTTTTTGC GTGAGCCATG
     6541 AGAACGAACC ATTGAGATCA TGCTTACTTT GCATGTCACT CAAAAATTTT GCCTCAAAAC
     6601 TGGTGAGCTG AATTTTTGCA GTTAAAGCAT CGTGTAGTGT TTTTCTTAGT CCGTTACGTA
     6661 GGTAGGAATC TGATGTAATG GTTGTTGGTA TTTTGTCACC ATTCATTTTT ATCTGGTTGT
     6721 TCTCAAGTTC GGTTACGAGA TCCATTTGTC TATCTAGTTC AACTTGGAAA ATCAACGTAT
     6781 CAGTCGGGCG GCCTCGCTTA TCAACCACCA ATTTCATATT GCTGTAAGTG TTTAAATCTT
     6841 TACTTATTGG TTTCAAAACC CATTGGTTAA GCCTTTTAAA CTCATGGTAG TTATTTTCAA
     6901 GCATTAACAT GAACTTAAAT TCATCAAGGC TAATCTCTAT ATTTGCCTTG TGAGTTTTCT
     6961 TTTGTGTTAG TTCTTTTAAT AACCACTCAT AAATCCTCAT AGAGTATTTG TTTTCAAAAG
     7021 ACTTAACATG TTCCAGATTA TATTTTATGA ATTTTTTTAA CTGGAAAAGA TAAGGCAATA
     7081 TCTCTTCACT AAAAACTAAT TCTAATTTTT CGCTTGAGAA CTTGGCATAG TTTGTCCACT
     7141 GGAAAATCTC AAAGCCTTTA ACCAAAGGAT TCCTGATTTC CACAGTTCTC GTCATCAGCT
     7201 CTCTGGTTGC TTTAGCTAAT ACACCATAAG CATTTTCCCT ACTGATGTTC ATCATCTGAG
     7261 CGTATTGGTT ATAAGTGAAC GATACCGTCC GTTCTTTCCT TGTAGGGTTT TCAATCGTGG
     7321 GGTTGAGTAG TGCCACACAG CATAAAATTA GCTTGGTTTC ATGCTCCGTT AAGTCATAGC
     7381 GACTAATCGC TAGTTCATTT GCTTTGAAAA CAACTAATTC AGACATACAT CTCAATTGGT
     7441 CTAGGTGATT TTAATCACTA TACCAATTGA GATGGGCTAG TCAATGATAA TTACTAGTCC
     7501 TTTTCCTTTG AGTTGTGGGT ATCTGTAAAT TCTGCTAGAC CTTTGCTGGA AAACTTGTAA
     7561 ATTCTGCTAG ACCCTCTGTA AATTCCGCTA GACCTTTGTG TGTTTTTTTT GTTTATATTC
     7621 AAGTGGTTAT AATTTATAGA ATAAAGAAAG AATAAAAAAA GATAAAAAGA ATAGATCCCA
     7681 GCCCTGTGTA TAACTCACTA CTTTAGTCAG TTCCGCAGTA TTACAAAAGG ATGTCGCAAA
     7741 CGCTGTTTGC TCCTCTACAA AACAGACCTT AAAACCCTAA AGGCTTAAGT AGCACCCTCG
     7801 CAAGCTCGGG CAAATCGCTG AATATTCCTT TTGTCTCCGA CCATCAGGCA CCTGAGTCGC
     7861 TGTCTTTTTC GTGACATTCA GTTCGCTGCG CTCACGGCTC TGGCAGTGAA TGGGGGTAAA
     7921 TGGCACTACA GGCGCCTTTT ATGGATTCAT GCAAGGAAAC TACCCATAAT ACAAGAAAAG
     7981 CCCGTCACGG GCTTCTCAGG GCGTTTTATG GCGGGTCTGC TATGTGGTGC TATCTGACTT
     8041 TTTGCTGTTC AGCAGTTCCT GCCCTCTGAT TTTCCAGTCT GACCACTTCG GATTATCCCG
     8101 TGACAGGTCA TTCAGACTGG CTAATGCACC CAGTAAGGCA GCGGTATCAT CAACAGGCTT
     8161 ACCCGTCTTA CTGTCCCTAG TGCTTGGATT CTCACCAATA AAAAACGCCC GGCGGCAACC
     8221 GAGCGTTCTG AACAAATCCA GATGGAGTTC TGAGGTCATT ACTGGATCTA TCAACAGGAG
//