LOCUS       C164                    5280 bp ds-DNA     circular     10-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1309..1466
                     /label="rrnb terminator"
                     /ApEinfo_revcolor=#cd0532
                     /ApEinfo_fwdcolor=#cd0532
     misc_feature    complement(2331..2919)
                     /label="PMB1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    386..403
                     /label="LacO"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    381..385
                     /label="-35"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     sig_peptide     1233..1265
                     /label="ssrA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_binding    1..23
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(4705..5269)
                     /label="Spacer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    519..1232
                     /label="superfolder YFP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(4459..4553)
                     /label="B0014terminator"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     gene            3041..3856
                     /label="Kan"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    404..409
                     /label="-10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1700..1901
                     /label="ROP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    443..508
                     /label="BCD"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    416..436
                     /label="LacO"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 ggaattgtga gcggataaca atttcacaca gggccctcgg acaccgagga gaatgtcaag
       61 aggcgaacac acaacgtctt ggagcgccag aggaggaacg agctaaaacg gagctttttt
      121 gccctgcgtg accagatccc ggagttggaa aacaatgaaa aggcccccaa ggtagttatc
      181 cttaaaaaag ccacagcata catcctgtcc gtccaagcag aggagcaaaa gctcatttct
      241 gaagaggact tgttgcggaa acgacgagaa cagttgaaac acaaacttga acagctacgg
      301 aactcttgtg cgtaaggaaa agtaaggaaa acgattcctt ctaacagaaa tgtcctgagc
      361 aatcacctat gaactgtcga TTGACATTGT GAGCGGATAA CAAGATACTG AGCACAATTG
      421 TGAGCGGATA ACAATTgcat gccacttaaa aaggagatca acaatgaaag caattttcgt
      481 actgaaacat cttaatcatg ctaaggagaa atactagtat gcgtaaaggc gaagagctgt
      541 tcactggtgt cgtccctatt ctggtggaac tggatggtga tgtcaacggt cataagtttt
      601 ccgtgcgtgg cgagggtgaa ggtgacgcaa ctaatggtaa actgacgctg aagttcatct
      661 gtactactgg taaactgccg gtaccttggc cgactctggt aacgacgctg acttatggtg
      721 ttcagtgctt tgctcgttat ccggaccata tgaagcagca tgacttcttc aagtccgcca
      781 tgccggaagg ctatgtgcag gaacgcacga tttcctttaa ggatgacggc acgtacaaaa
      841 cgcgtgcgga agtgaaattt gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca
      901 ttgactttaa agaagacggc aatatcctgg gccataagct ggaatacaat tttaacagcc
      961 acaatgttta catcaccgcc gataaacaaa aaaatggcat taaagcgaat tttaaaattc
     1021 gccacaacgt ggaggatggc agcgtgcagc tggctgatca ctaccagcaa aacactccaa
     1081 tcggtgatgg tcctgttctg ctgccagaca atcactatct gagcTACcaa agcgttctgt
     1141 ctaaagatcc gaacgagaaa cgcgatcata tggttctgct ggagttcgta accgcagcgg
     1201 gcatcacgca tggtatggat gaactgtaca aagcagcgaa cgacgaaaat tacgcccttg
     1261 cagcgtaata aGAGCTCgaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     1321 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     1381 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
     1441 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1501 tactagagtc ataagtgcgg aagcgctggc attgaccctg agtgattttt ctctggtccc
     1561 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
     1621 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
     1681 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca
     1741 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
     1801 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
     1861 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     1921 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     1981 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     2041 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg
     2101 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     2161 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2221 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2281 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2341 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2401 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2461 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2521 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2581 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2641 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2701 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2761 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2821 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2881 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2941 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaacaat
     3001 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     3061 aacgtcttgc tctaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     3121 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
     3181 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     3241 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     3301 tatccgtact cctgatgatg catggttact caccactgcg atccccggga aaacagcatt
     3361 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     3421 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     3481 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     3541 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     3601 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     3661 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     3721 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     3781 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     3841 cgatgagttt ttctaagaat taattcatga gcggatacat atttgaatgt atttagaaaa
     3901 ataaacaaat aggggttccg cgcacatttc cccgGCTAGC cgcctggtgc tacgcctgaa
     3961 taagtgataa taagcggatg aatggcagaa attcgaaagc aaattcgacc cggtcgtcgg
     4021 ttcagggcag ggtcgttaaa tagccgctta tgtctattgc tggtttaccg gtttattgac
     4081 taccggaagc agtgtgaccg tgtgcttctc aaatgcctga ggccagtttg ctcaggctct
     4141 ccccgtggag gtaataattg acgatatgat catttattct gcctcccaga gcctgataaa
     4201 aacggttagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
     4261 gcgatgcaga tccggaacat aatggtgcag ggcgcttgtt tcggcgtggg tatggtggca
     4321 ggccccgtgg ccgggggact gttgggcgct gccggcacct gtcctacgag ttgcatgata
     4381 aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagcta
     4441 ccgcctgcgg ccgctaggaa ataataaaaa agccggatta ataatctggc tttttatatt
     4501 ctctctctag tatataaacg cagaaaggcc cacccgaagg tgagccagtg tgagacagcg
     4561 gtgcggactg ttgtaactca gaataagaaa tgaggccgct catggcgttg actctcagtc
     4621 atagtatcgt ggtatcaccg gttggttcca ctctctgttg cgggcaactt cagcagcacg
     4681 taggggAAGC TTttattaCT CGAGaagcgc gtctgaatag gcttcttgta ttcctgccac
     4741 acagggaaac catcaggagt tacccaatgc acagcgcaac gcttgcgaag aatctctcca
     4801 gtcttcttat ctttgacctc agcagccagc agcttagcag cagacttaag ccagttcatt
     4861 gcttcaaccg cagctaccac cgtcacgctc acagattccc aaatcagctt agccatgtat
     4921 ccagcagcct gattcggctg agtgaacatc agacccttgc cggaatcaat agctggctga
     4981 atggtatctt ccagcacttg ttgacggaag ccgaactctt tggacccgta agccagcgtc
     5041 atgactgaac gcttagtcac actgcgagta acaccgtaag ccagccattg accagccagt
     5101 gccttagtgc ccagcttgac tttctcagag atttcaccag tgttctcatc ggtcacggta
     5161 actacttcgt tatcggtccc attgattgcg tctgcttgta gaatctcgtt gactttctta
     5221 gcaacaatcc cgtagatgtc ctgaacggtt tcactaggaa gcaagttaag acgtctgtgt
//