LOCUS       C308                    5299 bp ds-DNA     circular     10-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1420..1437
                     /label="SeqL-A (ATTL1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1437..1515
                     /label="BioBrick_B0010 Term"
                     /ApEinfo_revcolor=#eb0214
                     /ApEinfo_fwdcolor=#eb0214
     misc_feature    complement(2347..2935)
                     /label="PMB1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     sig_peptide     1249..1281
                     /label="ssrA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1516..1526
                     /label="B0010 lack part"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1448..1491
                     /label="rrnb_T1 terminator"
                     /ApEinfo_revcolor=#b90746
                     /ApEinfo_fwdcolor=#b90746
     misc_feature    420..425
                     /label="-10"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    535..1248
                     /label="superfolder GFP->CFP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(4475..4569)
                     /label="B0014terminator"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     misc_feature    967..969
                     /label="F145Y"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    397..402
                     /label="-35"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1..17
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    complement(4703..4708)
                     /label="HindIII"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    4721..5285
                     /label="Spacer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    378..396
                     /label="R0071 pRhl from pseudomonas PAO1"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    1288..1293
                     /label="SacI"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1716..1917
                     /label="ROP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    535..1248
                     /label="superfolder CFP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     gene            3057..3872
                     /label="Kan"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    complement(4721..5285)
                     /label="Spacer from T7"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(3951..3956)
                     /label="NheI"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    433..451
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    1325..1482
                     /label="rrnb terminator"
                     /ApEinfo_revcolor=#cd0532
                     /ApEinfo_fwdcolor=#cd0532
     misc_feature    4715..4720
                     /label="XhoI"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    970..972
                     /label="N146Y"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    403..419
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    459..524
                     /label="BCD"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    976..978
                     /label="H148D"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 tccctatcag tgatagagat tcacacaggg ccctcggaca ccgaggagaa tgtcaagagg
       61 cgaacacaca acgtcttgga gcgccagagg aggaacgagc taaaacggag cttttttgcc
      121 ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg cccccaaggt agttatcctt
      181 aaaaaagcca cagcatacat cctgtccgtc caagcagagg agcaaaagct catttctgaa
      241 gaggacttgt tgcggaaacg acgagaacag ttgaaacaca aacttgaaca gctacggaac
      301 tcttgtgcgt aaggaaaagt aaggaaaacg attccttcta acagaaatgt cctgagcaat
      361 cacctatgaa ctgtcgatcc tgtgaaatct ggcagtTTTA CTtccctatc agtgatagaG
      421 ATAATGAGCA Catccctatc agtgatagag atgcatgcca cttaaaaagg agatcaacaa
      481 tgaaagcaat tttcgtactg aaacatctta atcatgctaa ggagaaatac tagtatgcgt
      541 aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga tggtgatgtc
      601 aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg
      661 acgctgaagt tcatctgtac tactggtaaa ctgccggtac cttggccgac tctggtaacg
      721 acgctgactT GGggtgttca gtgctttgct cgttatccgg accatatgaa gcagcatgac
      781 ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc ctttaaggat
      841 gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc
      901 attgagctga aaggcattga ctttaaagaa gacggcaata tcctgggcca taagctggaa
      961 tacaatTACA TCagcGACaa tgtttacatc accgccgata aacaaaaaaa tggcattaaa
     1021 gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc tgatcactac
     1081 cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca ctatctgagc
     1141 acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt tctgctggag
     1201 ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaagc agcgaacgac
     1261 gaaaattacg cccttgcagc gtaataaGAG CTCgaacgca gaagcggtct gataaaacag
     1321 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
     1381 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
     1441 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1501 gtcggtgaac gctctctact agagtcataa gtgcggaagc gctggcattg accctgagtg
     1561 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
     1621 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
     1681 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
     1741 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
     1801 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
     1861 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     1921 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     1981 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     2041 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     2101 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     2161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2761 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3001 tttggtcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     3061 gccatattca acgggaaacg tcttgctcta ggccgcgatt aaattccaac atggatgctg
     3121 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     3181 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     3241 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     3301 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     3361 ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     3421 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     3481 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     3541 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     3601 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     3661 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     3721 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     3781 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     3841 agtttcattt gatgctcgat gagtttttct aagaattaat tcatgagcgg atacatattt
     3901 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg GCTAGCcgcc
     3961 tggtgctacg cctgaataag tgataataag cggatgaatg gcagaaattc gaaagcaaat
     4021 tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc tattgctggt
     4081 ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggcc
     4141 agtttgctca ggctctcccc gtggaggtaa taattgacga tatgatcatt tattctgcct
     4201 cccagagcct gataaaaacg gttagcgctt cgttaataca gatgtaggtg ttccacaggg
     4261 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg cttgtttcgg
     4321 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgctgccg gcacctgtcc
     4381 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
     4441 ccaccggaag gagctaccgc ctgcggccgc taggaaataa taaaaaagcc ggattaataa
     4501 tctggctttt tatattctct ctctagtata taaacgcaga aaggcccacc cgaaggtgag
     4561 ccagtgtgag acagcggtgc ggactgttgt aactcagaat aagaaatgag gccgctcatg
     4621 gcgttgactc tcagtcatag tatcgtggta tcaccggttg gttccactct ctgttgcggg
     4681 caacttcagc agcacgtagg ggAAGCTTtt attaCTCGAG aagcgcgtct gaataggctt
     4741 cttgtattcc tgccacacag ggaaaccatc aggagttacc caatgcacag cgcaacgctt
     4801 gcgaagaatc tctccagtct tcttatcttt gacctcagca gccagcagct tagcagcaga
     4861 cttaagccag ttcattgctt caaccgcagc taccaccgtc acgctcacag attcccaaat
     4921 cagcttagcc atgtatccag cagcctgatt cggctgagtg aacatcagac ccttgccgga
     4981 atcaatagct ggctgaatgg tatcttccag cacttgttga cggaagccga actctttgga
     5041 cccgtaagcc agcgtcatga ctgaacgctt agtcacactg cgagtaacac cgtaagccag
     5101 ccattgacca gccagtgcct tagtgcccag cttgactttc tcagagattt caccagtgtt
     5161 ctcatcggtc acggtaacta cttcgttatc ggtcccattg attgcgtctg cttgtagaat
     5221 ctcgttgact ttcttagcaa caatcccgta gatgtcctga acggtttcac taggaagcaa
     5281 gttaagacgt ctgtgtgga
//