LOCUS       C309                    3984 bp ds-DNA     circular     10-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    420..425
                     /label="-10"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    397..402
                     /label="-35"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     sig_peptide     1249..1281
                     /label="ssrA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    403..419
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    2547..3135
                     /label="PMB1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(3565..3766)
                     /label="ROP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    433..451
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    1427..1521
                     /label="B0014terminator"
                     /ApEinfo_revcolor=#808040
                     /ApEinfo_fwdcolor=#808040
     misc_feature    1..17
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    459..524
                     /label="BCD"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     gene            complement(1610..2425)
                     /label="Kan"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    311..396
                     /label="pCin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    535..1248
                     /label="superfolder YFP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 tccctatcag tgatagagat tcacacaggg ccctcggaca ccgaggagaa tgtcaagagg
       61 cgaacacaca acgtcttgga gcgccagagg aggaacgagc taaaacggag cttttttgcc
      121 ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg cccccaaggt agttatcctt
      181 aaaaaagcca cagcatacat cctgtccgtc caagcagagg agcaaaagct catttctgaa
      241 gaggacttgt tgcggaaacg acgagaacag ttgaaacaca aacttgaaca gctacggaac
      301 tcttgtgcgt ggcgcatgtc gttatcgcaa aaccgctgca cacttttgcg cgacatgctc
      361 tgatccccct catctggggg ggcctatctg agggaaTTTA CTtccctatc agtgatagaG
      421 ATAATGAGCA Catccctatc agtgatagag atgcatgcca cttaaaaagg agatcaacaa
      481 tgaaagcaat tttcgtactg aaacatctta atcatgctaa ggagaaatac tagtatgcgt
      541 aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga tggtgatgtc
      601 aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg
      661 acgctgaagt tcatctgtac tactggtaaa ctgccggtac cttggccgac tctggtaacg
      721 acgctgactt atggtgttca gtgctttgct cgttatccgg accatatgaa gcagcatgac
      781 ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc ctttaaggat
      841 gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc
      901 attgagctga aaggcattga ctttaaagaa gacggcaata tcctgggcca taagctggaa
      961 tacaatttta acagccacaa tgtttacatc accgccgata aacaaaaaaa tggcattaaa
     1021 gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc tgatcactac
     1081 cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca ctatctgagc
     1141 TACcaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt tctgctggag
     1201 ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaagc agcgaacgac
     1261 gaaaattacg cccttgcagc gtaataaAAG CTTcccctac gtgctgctga agttgcccgc
     1321 aacagagagt ggaaccaacc ggtgatacca cgatactatg actgagagtc aacgccatga
     1381 gcggcctcat ttcttattct gagttacaac agtccgcacc gctgtctcac actggctcac
     1441 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt
     1501 aatccggctt ttttattatt tcctaGCTAG Ccggggaaat gtgcgcggaa cccctatttg
     1561 tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact
     1621 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     1681 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     1741 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     1801 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     1861 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     1921 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     1981 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     2041 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     2101 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     2161 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     2221 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     2281 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     2341 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag
     2401 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     2461 gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2521 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2581 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2641 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     2701 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2761 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2821 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2881 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2941 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3001 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3061 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3121 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3181 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3241 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3301 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3361 tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     3421 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     3481 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3541 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3601 gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc
     3661 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg
     3721 ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt
     3781 tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac
     3841 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat
     3901 gcggcgggac caGAGCTCag tgcgCTCGAG cttcccaacc ttaccagagg gcgccccagc
     3961 tggcaattcc gacgtctgtg tgga
//