LOCUS       pET-14b-His-Zdk2-Cofilin-LOV        5782 bp    DNA     circular
            
ACCESSION   DNA_pET-14b-SUMO-BlkSH3
VERSION     DNA_pET-14b-SUMO-BlkSH3.0
KEYWORDS    .

COMMENT
DISPLAY_SETUP_START|
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                        <font name="Georgia" size="8" style="0"/>
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                <location x="432" y="547"/>
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                <style name="RSite Label" xsi:type="basicStyle">
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                <location x="104" y="237"/>
                <dimension height="312" width="312"/>
            </element>
            <element id="751297011" target="751297011" text="@L bp"
                type="-2055714368" xsi:type="label">
                <style name="Molecule Label 2" xsi:type="basicStyle">
                    <label text="@L bp">
                        <font name="Arial" size="18" style="0"/>
                        <color blue="50" green="50" red="50"/>
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                </style>
                <location x="230" y="397"/>
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            <element id="1300314975" target="2055105" type="2055105" xsi:type="symbol">
                <style name="Axis" xsi:type="basicStyle">
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                        <color blue="153" green="204" red="204"/>
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                <location x="89" y="369"/>
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            <element id="1564510445" name="T7 Term Rev"
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                <location x="54" y="187"/>
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                <location x="29" y="162"/>
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                        <font name="Arial" size="9" style="1"/>
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                <location x="460" y="268"/>
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            <element id="128286974" name="Full" target="Full:100 - 1278"
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                <location x="415" y="212"/>
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                <location x="79" y="212"/>
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            <element id="471447175" name="Zdk2" target="Zdk2:136 - 313"
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                <location x="311" y="157"/>
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    </setupInfo>
    <setupInfo complete="true" completeMinSize="150"
        completeNested="false" includeStopCodon="false"
        incomplete="false" incompleteMinSize="30" selected="false"
        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
        undefinedStart="false" undefinedStop="false" undefinedStopNested="false"/>
    <setupInfo allowBlunt="true" allowFivePrime="true"
        allowThreePrime="true"
        enzymeMap="BamHI|ggatcc,ClaI|atcgat,AvaI|cycgrg,EcoRI|gaattc,XmaI|cccggg,SmaI|cccggg,ApaLI|gtgcac,HindIII|aagctt,NcoI|ccatgg,PstI|ctgcag"
        enzymesCleavePoint="BamHI|2,ClaI|3,AvaI|2,EcoRI|2,XmaI|2,SmaI|4,ApaLI|2,HindIII|2,NcoI|2,PstI|6"
        enzymesComplCleavePoint="BamHI|0,ClaI|0,AvaI|0,EcoRI|0,XmaI|0,SmaI|0,ApaLI|0,HindIII|0,NcoI|0,PstI|0"
        enzymesPalindromic="BamHI|true,ClaI|true,AvaI|true,EcoRI|true,XmaI|true,SmaI|true,ApaLI|true,HindIII|true,NcoI|true,PstI|true"
        ignoreIn="-1,-1" ignoreLess="-1" ignoreMore="-1"
        ignoreOut="-1,-1" selected="true" startIndex="1" type="Restriction Map"/>
    <setupInfo selected="true" type="PictureType"
        xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:type="pictureTypeSetup"/>
    <setupInfo featureList="" selected="true" type="Feature Map"/>
    <setupInfo basesPerBlock="10" blocksPerLine="10"
        prefer1LetterAACode="true" preferSingleStrand="false"
        selected="true" textBold="false" textColor="0,0,0"
        textFontName="Andale Mono" textFontSize="14" textItalic="false"
        textUnderline="false" trackSeparatorColor="128,128,128"
        trackSeparatorWidth="2" type="Sequence"
        xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:type="sequenceSetup">
        <textRangeSetupList>
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                startIndex="1093" stopIndex="1101" underline="false"/>
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                fontName="" fontSize="0" italic="false" startIndex="427"
                stopIndex="432" underline="false"/>
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                fontName="" fontSize="0" italic="false" startIndex="179"
                stopIndex="187" underline="false"/>
            <textRangeSetup backgroundColor="255,102,102" bold="false"
                fontName="" fontSize="0" italic="false" startIndex="178"
                stopIndex="178" underline="false"/>
            <textRangeSetup backgroundColor="255,102,102" bold="false"
                fontName="" fontSize="0" italic="false" startIndex="188"
                stopIndex="426" underline="false"/>
            <textRangeSetup backgroundColor="179,179,179" bold="false"
                fontName="" fontSize="0" italic="false" startIndex="112"
                stopIndex="129" underline="false"/>
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                fontName="" fontSize="0" italic="false" startIndex="478"
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    <setupInfo analysisSubsets="Analysis Results (MAIN)" selected="true" type="Auto Load Analysis"/>
</vntiDisplaySetup>
DISPLAY_SETUP_END|
COMMENT     
            VNTSEQSTYLE|472|477||0|null|null|null|255,102,255|null
            VNTSEQSTYLE|1093|1101||0|null|null|null|255,111,207|null
            VNTSEQSTYLE|427|432||0|null|null|null|255,102,102|null
            VNTSEQSTYLE|179|187||0|null|null|null|255,102,102|null
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            VNTSEQSTYLE|188|426||0|null|null|null|255,102,102|null
            VNTSEQSTYLE|112|129||0|null|null|null|179,179,179|null
            VNTSEQSTYLE|478|480||0|null|null|null|255,255,0|null
            VNTSEQSTYLE|481|1092||0|null|null|null|0,255,0|null
FEATURES             Location/Qualifiers
     misc_feature    112..129
                     /label=HisTag
                     /vntifkey="21"
     misc_feature    136..313
                     /label=Zdk2
                     /type="misc_feature"
     misc_feature    328..825
                     /label=Cofilin
                     /type="misc_feature"
     misc_feature    850..1275
                     /label=LOV
                     /type="misc_feature"
     misc_feature    21..40
                     /label=T7 Promotor
                     /type="misc_feature"
     misc_feature    1329..1347
                     /label=T7 Term Rev
                     /type="misc_feature"
     misc_feature    959..979
                     /label=LOV Rev
                     /type="misc_feature"
     misc_feature    527..546
                     /label=Cofilin Rev
                     /type="misc_feature"
     misc_feature    100..1278
                     /label=Full
                     /type="misc_feature"
ORIGIN
        1 agatctcgat cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc
       61 tagaaataat tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat
      121 catcatcacg gtaccatggt ggataacaaa ttcaataaag aaatgttgag tgcccgtgtg
      181 gaaatctatg gtctgccaaa cctgaattgg ggtcagaggt ttgccttcat cagtagcctg
      241 acggatgatc catctcagag cgccaatctg ctggccgaag ccaaaaaact gaacgatgcc
      301 caggccccaa aaggttctgg aggcggaatg gcctctggtg tggctgtctc tgatggagtc
      361 atcaaggtgt tcaatgacat gaaagttcgc aagtcttcaa cgccagaaga agtgaagaaa
      421 cgcaagaagg cagtgctctt ttgcctgagt gaggacaaga agaacatcat cctggaggag
      481 ggcaaggaga ttctggtagg agatgtgggg cagactgtgg acgaccccta caccactttt
      541 gtcaagatgc tgccagacaa ggactgccgc tatgctctct atgacgcaac ctatgagacc
      601 aaggagagca agaaggagga cctggtattc attttctggg cccccgagag tgcacccctt
      661 aagagcaaaa tgatctatgc cagctccaag gatgccatca agaagaaact gacaggaatc
      721 aagcacgaat tacaagctaa ctgctacgag gaggtcaagg accgctgcac cctggcagag
      781 aaactaggtg gcagcgccgt catttccctg gagggcaagc ctttgggcgg tagcggaggg
      841 agcggcggtg ctactacact tgaacgtatt gagaagaact ttgtcattac tgacccaaga
      901 ttgccagata atcccattat attcgcgtcc gatagtttct tgcagttgac agaatatagc
      961 cgtgaagaaa ttttgggaag aaactgcagg tttctacaag gtcctgaaac tgatcgcgcg
     1021 acagtgagaa aaattagaga tgccatagat aaccaaacag aggtcactgt tcagctgatt
     1081 aattatacaa agagtggtaa aaagttctgg aacctctttc acttgcagcc tatgcgagat
     1141 cagaagggag atgtccagta ctttattggg gttcagttgg atggaactga gcatgtccga
     1201 gatgctgccg agagagaggg agtcatgctg attaagaaaa ctgcagaaaa tattgatgag
     1261 gcggcaaaag aactttaagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     1321 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     1381 gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca
     1441 tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa
     1501 agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg
     1561 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc
     1621 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga
     1681 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact
     1741 gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga
     1801 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1861 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1921 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1981 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2041 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2101 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2161 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2221 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     2281 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2341 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2401 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2461 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2521 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     2581 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2641 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2701 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2761 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2821 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2881 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2941 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3001 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3061 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3121 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3181 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3241 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3301 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3361 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3421 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3481 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3541 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3601 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3661 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3721 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3781 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     3841 ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3901 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     3961 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4021 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4081 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
     4141 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
     4201 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     4261 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
     4321 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
     4381 atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac
     4441 agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat
     4501 ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat
     4561 tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg
     4621 tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa
     4681 cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg
     4741 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
     4801 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
     4861 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
     4921 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
     4981 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg
     5041 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
     5101 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
     5161 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc
     5221 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
     5281 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     5341 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     5401 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     5461 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc
     5521 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     5581 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     5641 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     5701 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     5761 atgcgtccgg cgtagaggat cg
//
