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DISPLAY_SETUP_START|
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                        <color blue="0" green="0" red="0"/>
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                <location x="444" y="305"/>
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            <element id="1576634369" name="BglII (1968)"
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                <location x="139" y="39"/>
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            <element id="1389387336" name="BstBI (65)"
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                <location x="295" y="5"/>
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                <location x="139" y="39"/>
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                <location x="265" y="199"/>
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                <location x="117" y="171"/>
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                <location x="399" y="342"/>
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                        <color blue="153" green="204" red="102"/>
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                <location x="114" y="14"/>
                <dimension height="362" width="362"/>
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            <element id="961649911" name="Zdk2"
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                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
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                <location x="307" y="389"/>
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            <element id="961649911" name="Zdk2"
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                        <color blue="153" green="204" red="102"/>
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                </style>
                <location x="114" y="14"/>
                <dimension height="362" width="362"/>
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            <element id="907662423" name="CMV primer"
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                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
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                <location x="456" y="276"/>
            </element>
            <element id="907662423" name="CMV primer"
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            <element id="907662423" name="CMV primer" shape="1"
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                        <pattern style="1"/>
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                    <fill pattern="Solid">
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                </style>
                <location x="114" y="14"/>
                <dimension height="362" width="362"/>
            </element>
            <element id="743420481" name="EBV Rev (SV40 polyA term)"
                target="EBV Rev (SV40 polyA term):4057 - 4076" text="@N"
                type="21" xsi:type="label">
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                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
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                <location x="5" y="251"/>
            </element>
            <element id="743420481" name="EBV Rev (SV40 polyA term)"
                target="EBV Rev (SV40 polyA term):4057 - 4076" type="21" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
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                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
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            </element>
            <element id="743420481" name="EBV Rev (SV40 polyA term)"
                shape="1" target="EBV Rev (SV40 polyA term):4057 - 4076"
                type="21" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
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                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="114" y="14"/>
                <dimension height="362" width="362"/>
            </element>
            <element id="1643078777" name="Cofilin"
                target="Cofilin:2894 - 3391" text="@N" type="21" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="223" y="370"/>
            </element>
            <element id="1643078777" name="Cofilin"
                target="Cofilin:2894 - 3391" type="21" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
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            </element>
            <element id="1643078777" name="Cofilin" shape="1"
                target="Cofilin:2894 - 3391" type="21" xsi:type="symbol">
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                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
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                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="114" y="14"/>
                <dimension height="362" width="362"/>
            </element>
            <element id="472812309" name="LOV2"
                target="LOV2:3416 - 3841" text="@N" type="21" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
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                </style>
                <location x="145" y="324"/>
            </element>
            <element id="472812309" name="LOV2"
                target="LOV2:3416 - 3841" type="21" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
                    </line>
                </style>
            </element>
            <element id="472812309" name="LOV2" shape="1"
                target="LOV2:3416 - 3841" type="21" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="114" y="14"/>
                <dimension height="362" width="362"/>
            </element>
        </elements>
    </setupInfo>
    <setupInfo complete="true" completeMinSize="150"
        completeNested="false" includeStopCodon="false"
        incomplete="false" incompleteMinSize="30" selected="false"
        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
        undefinedStart="false" undefinedStop="false" undefinedStopNested="false"/>
    <setupInfo allowBlunt="true" allowFivePrime="true"
        allowThreePrime="true"
        enzymeMap="KpnI|ggtacc,AscI|ggcgcgcc,ApaI|gggccc,AseI|attaat,EcoRV|gatatc,HpaI|gttaac,PspOMI|gggccc,StuI|aggcct,EcoRI|gaattc,NotI|gcggccgc,SacI|gagctc,SfiI|ggccnnnnnggcc,AgeI|accggt,BglII|agatct,MluI|acgcgt,HindIII|aagctt,MfeI|caattg,BspEI|tccgga,BsrGI|tgtaca,BseRI|gaggag,EagI|cggccg,PstI|ctgcag,ScaI|agtact,BamHI|ggatcc,SapI|gctcttc,BclI|tgatca,XhoI|ctcgag,SmaI|cccggg,BsaI|ggtctc,SpeI|actagt,SacII|ccgcgg,DraIII|cacnnngtg,NcoI|ccatgg,BbvCI|cctcagc,SalI|gtcgac,BssHII|gcgcgc,ClaI|atcgat,PvuII|cagctg,BstBI|ttcgaa,PacI|ttaattaa,XmaI|cccggg,PciI|acatgt,SphI|gcatgc,NheI|gctagc,XbaI|tctaga,NdeI|catatg"
        enzymesCleavePoint="KpnI|6,AscI|3,ApaI|6,AseI|3,EcoRV|4,HpaI|4,PspOMI|2,StuI|4,EcoRI|2,NotI|3,SacI|6,SfiI|9,AgeI|2,BglII|2,MluI|2,HindIII|2,MfeI|2,BspEI|2,BsrGI|2,BseRI|17,EagI|2,PstI|6,ScaI|4,BamHI|2,SapI|9,BclI|2,XhoI|2,SmaI|4,BsaI|8,SpeI|2,SacII|5,DraIII|7,NcoI|2,BbvCI|3,SalI|2,BssHII|2,ClaI|3,PvuII|4,BstBI|3,PacI|6,XmaI|2,PciI|2,SphI|6,NheI|2,XbaI|2,NdeI|3"
        enzymesComplCleavePoint="KpnI|0,AscI|0,ApaI|0,AseI|0,EcoRV|0,HpaI|0,PspOMI|0,StuI|0,EcoRI|0,NotI|0,SacI|0,SfiI|0,AgeI|0,BglII|0,MluI|0,HindIII|0,MfeI|0,BspEI|0,BsrGI|0,BseRI|15,EagI|0,PstI|0,ScaI|0,BamHI|0,SapI|12,BclI|0,XhoI|0,SmaI|0,BsaI|12,SpeI|0,SacII|0,DraIII|0,NcoI|0,BbvCI|6,SalI|0,BssHII|0,ClaI|0,PvuII|0,BstBI|0,PacI|0,XmaI|0,PciI|0,SphI|0,NheI|0,XbaI|0,NdeI|0"
        enzymesPalindromic="KpnI|true,AscI|true,ApaI|true,AseI|true,EcoRV|true,HpaI|true,PspOMI|true,StuI|true,EcoRI|true,NotI|true,SacI|true,SfiI|true,AgeI|true,BglII|true,MluI|true,HindIII|true,MfeI|true,BspEI|true,BsrGI|true,BseRI|false,EagI|true,PstI|true,ScaI|true,BamHI|true,SapI|false,BclI|true,XhoI|true,SmaI|true,BsaI|false,SpeI|true,SacII|true,DraIII|true,NcoI|true,BbvCI|false,SalI|true,BssHII|true,ClaI|true,PvuII|true,BstBI|true,PacI|true,XmaI|true,PciI|true,SphI|true,NheI|true,XbaI|true,NdeI|true"
        ignoreIn="-1,-1" ignoreLess="1" ignoreMore="1" ignoreOut="-1,-1"
        selected="true" startIndex="1" type="Restriction Map"/>
    <setupInfo selected="true" type="PictureType"
        xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:type="pictureTypeSetup"/>
    <setupInfo featureList="" selected="true" type="Feature Map"/>
    <setupInfo basesPerBlock="10" blocksPerLine="10"
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        selected="true" textBold="false" textColor="0,0,0"
        textFontName="Andale Mono" textFontSize="14" textItalic="false"
        textUnderline="false" trackSeparatorColor="128,128,128"
        trackSeparatorWidth="2" type="Sequence"
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    </setupInfo>
    <setupInfo analysisSubsets="Analysis Results (MAIN)" selected="true" type="Auto Load Analysis"/>
</vntiDisplaySetup>
DISPLAY_SETUP_END|
LOCUS       pmCherry-C1-Zdk2-Cofilin-LOV        5820 bp    DNA     circular
            
ACCESSION   DNA_pmCherry-C1-Zdk2-Cofilin-LOV
VERSION     DNA_pmCherry-C1-Zdk2-Cofilin-LOV.0
KEYWORDS    .
FEATURES             Location/Qualifiers
     misc_feature    1982..2689
                     /label=mCherry
                     /type="misc_feature"
                     /vntifkey="21"
     misc_feature    2894..3391
                     /label=Cofilin
                     /type="misc_feature"
                     /vntifkey="21"
     misc_feature    2705..2879
                     /label=Zdk2
                     /type="misc_feature"
                     /vntifkey="21"
     misc_feature    3416..3841
                     /label=LOV2
                     /type="misc_feature"
                     /vntifkey="21"
     misc_feature    1877..1897
                     /label=CMV primer
                     /type="misc_feature"
                     /vntifkey="21"
     misc_feature    4057..4076
                     /label=EBV Rev (SV40 polyA term)
                     /type="misc_feature"
                     /vntifkey="21"
ORIGIN
        1 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
       61 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
      121 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
      181 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
      241 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
      301 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
      361 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
      421 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
      481 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
      541 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
      601 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      661 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      721 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      781 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
      841 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      901 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      961 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1021 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1081 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1141 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1201 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1261 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1321 cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt
     1381 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     1441 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     1501 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     1561 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     1621 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     1681 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     1741 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     1801 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     1861 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     1921 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat ctcgagccac
     1981 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     2041 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     2101 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     2161 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     2221 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     2281 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     2341 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     2401 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     2461 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     2521 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     2581 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     2641 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gaagcggtag
     2701 tggagtggat aacaaattca ataaagaaat gttgagtgcc cgtgtggaaa tctatggtct
     2761 gccaaacctg aattggggtc agaggtttgc cttcatcagt agcctgacgg atgatccatc
     2821 tcagagcgcc aatctgctgg ccgaagccaa aaaactgaac gatgcccagg ccccaaaagg
     2881 ttctggaggc ggaatggcct ctggtgtggc tgtctctgat ggagtcatca aggtgttcaa
     2941 tgacatgaaa gttcgcaagt cttcaacgcc agaagaagtg aagaaacgca agaaggcagt
     3001 gctcttttgc ctgagtgagg acaagaagaa catcatcctg gaggagggca aggagattct
     3061 ggtaggagat gtggggcaga ctgtggacga cccctacacc acttttgtca agatgctgcc
     3121 agacaaggac tgccgctatg ctctctatga cgcaacctat gagaccaagg agagcaagaa
     3181 ggaggacctg gtattcattt tctgggcccc cgagagtgca ccccttaaga gcaaaatgat
     3241 ctatgccagc tccaaggatg ccatcaagaa gaaactgaca ggaatcaagc acgaattaca
     3301 agctaactgc tacgaggagg tcaaggaccg ctgcaccctg gcagagaaac taggtggcag
     3361 cgccgtcatt tccctggagg gcaagccttt gggcggtagc ggagggagcg gcggtgctac
     3421 tacacttgaa cgtattgaga agaactttgt cattactgac ccaagattgc cagataatcc
     3481 cattatattc gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt
     3541 gggaagaaac tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat
     3601 tagagatgcc atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag
     3661 tggtaaaaag ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt
     3721 ccagtacttt attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag
     3781 agagggagtc atgctgatta agaaaactgc agaaaatatt gatgaggcgg caaaagaact
     3841 ttaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
     3901 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3961 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4021 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4081 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     4141 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     4201 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     4261 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     4321 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     4381 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     4441 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     4501 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     4561 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4621 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4681 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4741 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4801 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4861 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4921 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4981 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     5041 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5101 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5161 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5221 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     5281 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5341 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5401 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5461 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5521 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5581 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     5641 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5701 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5761 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
//
