LOCUS       pWRKY70_LUC             6800 bp ds-DNA     circular     13-MAR-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 13, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(15..31)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label=M13 rev
                     /ApEinfo_fwdcolor=#31878a
                     /ApEinfo_revcolor=#4ec02b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    39..55
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label=lac operator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(63..93)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /label=lac promoter
                     /ApEinfo_fwdcolor=#346ee0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label=ori
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(1176..2036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     I
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     S
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     W
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     A
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     S LIKHW"
                     /label=AmpR
                     /ApEinfo_fwdcolor=#e9d024
                     /ApEinfo_revcolor=#e9d024
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(2037..2141)
                     /gene="bla"
                     /label=AmpR promoter
                     /ApEinfo_fwdcolor=#346ee0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2615..2631
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label=M13 fwd
                     /ApEinfo_fwdcolor=#31878a
                     /ApEinfo_revcolor=#4ec02b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2667..4734
                     /label=AtWRKY70 promoter
                     /ApEinfo_fwdcolor=#346ee0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             4737..6389
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /note="luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     A
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVA
                     P
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQ
                     S
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSH
                     A
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDY
                     K
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQG
                     Y
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIM
                     S
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESI
                     L
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGV
                     V FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
                     /label=firefly luciferase
                     /ApEinfo_fwdcolor=#e9d024
                     /ApEinfo_revcolor=#e9d024
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      6544..6795
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
                     /label=NOS terminator
                     /ApEinfo_fwdcolor=#9d1b1c
                     /ApEinfo_revcolor=#9d1b1c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
       61 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      841 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2161 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2401 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     2461 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     2521 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     2581 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
     2641 gcatgcctgc aggctctaga ggatcccaac tggtttatca tagtatctcg catattaact
     2701 taggctagag agccaacctt tttgaattaa tgaaaaagtt gagacacaac cactaaactt
     2761 tacggcatta ataacggatc tcatttatga acctgcgtac aaatttggtc tgaagttaag
     2821 tggtactatc ccggaaataa cttaaatctt atcctaaact atattctttc cattttatag
     2881 tacgagatgt tttggaaaaa aaaacaaata gtttcataat aaagtaatat tttcaagttc
     2941 ttatgcaata ttttatgatt agttattagt tatattatgc attttcttta tgtttggttg
     3001 aactttttaa aaatatgtaa tttttaatat acgtgttttt agttaaacat cttatatttt
     3061 aaaacggatt aagtatcttt ttagtcataa tttatcttta tacacaaaaa agtaatactt
     3121 tgtcgattct taacccatgc aattgtttat gtgttctgaa attgattgat atgatcctag
     3181 ctcaactggc gttaatagac ttcgtatgaa gggaaaaaat ttcaaaatca ttaatttaaa
     3241 agttcgacgt gaaaaaatgt aataatttaa cattctctta taatgcttcc gtaagataaa
     3301 tttatggact attatccgac tttttgataa tgggtatgca aaaacttgtc atatctaaaa
     3361 aaaatatcac caatgttaat ttatacttgg gcataaatgt atcattaaaa aaaccttggt
     3421 ggcttggtgc atatagaaga gagtgaggct gtgagggagc actatagacc aactagacat
     3481 ctctaagcga ggagaaccca tccgcatcgc atgtatgcat tgagatagct agggcctagg
     3541 ggccattgag tcgatcgacc acatacacct ctttaaataa tcttctaata ttaaagtttt
     3601 aaatcaatgc cccgcccaac tctacctttt ccacgaaaac ggaataaagt gtttccgggt
     3661 gaaagaaaat acttttcaac gatagattgt gttgctattt ttatagaata tgttaaattt
     3721 aagtataata taagataggt tcctataaaa aaattgggta gaaaagttta gctttgatac
     3781 tccatatagt ttatatatta gtgcagcgag gtgatacgtt tactagtcta acttaacaaa
     3841 aaaaaaagag ttaattagtt tatatattga ttctttcaac gtaatcaatc tgatttagat
     3901 aatccgtccc aactcccaat tatatctgtc attcaagtat ttgattacaa gggcaattca
     3961 tttcggacta gttgattgta aatttcgtgt ctcgttatct atttgaaata gtaggtctat
     4021 ttagggggta tacatctgga ttgatagctg ataaggacta tatacatgtc tttttgaatt
     4081 tttgatatat aaactactat tcaaaattat cttcatagaa aaacggatca tatcatttgg
     4141 atatcaaatt taactcgaga tttcaaataa ataattcact attccaactg atcttactac
     4201 atcagtaaaa aaaaattggt agtacaatct cgaggaacaa aaaagtttac aaatgaaatt
     4261 tagaatcttg tttaatcttt aaatggatag tttttcataa ctcttttaat tagtctagtt
     4321 tttgatacaa attttatccg gttatagaaa acattaaagg accctaagtt tggatttcag
     4381 caaaattata tatattccat tctcacttag ggatgaaatg acatcattca tcactacgtc
     4441 atttgagctt acgtcaagct caccataagc aaaagaaatg ggtggataag ttcaagttcc
     4501 atctcatttc ctcacacatc tctctttctt ccttctcatt tcctcgcatc ttcctctaaa
     4561 aaaaaaaatc aaagaaacca acaaaataaa aagtgcccat cattacaaat cttatttcca
     4621 ccttatattt acacacactt ctcttctttc ctttaatcat acaaaccaca ccaagaggaa
     4681 agaaaaataa gataccactc accaaaaact tcctcaaaac taacaaatgg atacccatgg
     4741 aagacgccaa aaacataaag aaaggcccgg cgccattcta tcctctagag gatggaaccg
     4801 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     4861 ttacagatgc acatatcgag gtgaacatca cgtacgcgga atacttcgaa atgtccgttc
     4921 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
     4981 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
     5041 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg aacatttcgc
     5101 agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
     5161 aattaccaat aatccagaaa attattatca tggattctaa aacggattac cagggatttc
     5221 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
     5281 taccagagtc ctttgatcgt gacaaaacaa ttgcactgat aatgaattcc tctggatcta
     5341 ctgggttacc taagggtgtg gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg
     5401 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     5461 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     5521 tcgtcttaat gtatagattt gaagaagagc tgtttttacg atcccttcag gattacaaaa
     5581 ttcaaagtgc gttgctagta ccaaccctat tttcattctt cgccaaaagc actctgattg
     5641 acaaatacga tttatctaat ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag
     5701 aagtcgggga agcggttgca aaacgcttcc atcttccagg gatacgacaa ggatatgggc
     5761 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     5821 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     5881 tgggcgttaa tcagagaggc gaattatgtg tcagaggacc tatgattatg tccggttatg
     5941 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     6001 acatagctta ctgggacgaa gacgaacact tcttcatagt tgaccgcttg aagtctttaa
     6061 ttaaatacaa aggatatcag gtggcccccg ctgaattgga atcgatattg ttacaacacc
     6121 ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga tgacgccggt gaacttcccg
     6181 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     6241 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     6301 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     6361 ccaagaaggg cggaaagtcc aaattgtaaa atgtaactgt attcagcgat gacgaaattc
     6421 ttagctattg taatattata tgcaaattga tgaatggtaa ttttgtaatt gtgggtcact
     6481 gtactatttt aacgaataat aaaatcaggt ataggtaact aaaaaggaat tcgagctcga
     6541 attgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     6601 gcgatgatta tcatataatt tctgttgatt acgttaagca tgtaataatt aacatgtaat
     6661 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     6721 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     6781 ctatgttact agatctctag    
//
