LOCUS       Exported                4240 bp ds-DNA     circular SYN 08-FEB-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMP89b-CVTA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 17, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             70..92
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             112..129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             133..165
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             172..192
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      1655..1702
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1799..2254
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2280..2384
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2385..3245
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3416..4004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
      121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tgagaaccta
      181 tatttccagg gggatccgag ctcgagtatg cagaagcaac gtacgaccag ccaatggcgc
      241 gaactggatg ccgcccatca cctgcatccg ttcaccgata ccgcatcgct gaaccaggcg
      301 ggcgcgcgcg tgatgacgcg cggagagggc gtctacctgt gggattcgga aggcaacaag
      361 atcatcgacg gcatggccgg actgtggtgc gtgaacgtcg gctacggccg caaggacttt
      421 gccgaagcgg cgcgccggca gatggaagag ctgccgttct acaacacctt cttcaagacc
      481 acccatccgg cggtggtcga gctgtccagc ctgctggctg aagtgacgcc ggccggtttc
      541 gaccgcgtgt tctataccaa ttccggttcc gaatcggtgg acaccatgat ccgcatggtg
      601 cgccgctact gggacgtgca gggcaagccg gagaagaaga cgctgatcgg ccgctggaac
      661 ggctatcacg gctccaccat cggcggcgcc agcctgggcg gcatgaagta catgcacgag
      721 cagggcgact tgccgattcc gggcatggcc cacatcgagc agccttggtg gtacaagcac
      781 ggcaaggaca tgacgccgga cgagttcggc gtggtggccg cgcgctggct ggaagagaag
      841 attctggaaa tcggcgccga caaggtggcc gccttcgtcg gcgaacccat ccagggcgcc
      901 ggcggcgtga tcgtcccgcc ggccacctac tggccggaaa tcgagcgcat ttgccgcaag
      961 tacgacgtgc tgctggtggc cgacgaagtg atctgcggct tcgggcgtac cggcgaatgg
     1021 ttcggccatc agcatttcgg cttccagccc gacctgttca ccgccgccaa gggcctgtcc
     1081 tccggctatc tgccgatagg cgcggtcttt gtcggcaagc gcgtggccga aggcctgatc
     1141 gccggcggcg acttcaacca cggcttcacc tactccggcc acccggtctg cgccgccgtc
     1201 gcccacgcca acgtggcggc gctgcgcgac gagggcatcg tccagcgcgt caaggacgac
     1261 atcggcccgt acatgcaaaa gcgctggcgt gaaaccttca gccgtttcga gcatgtggac
     1321 gacgtgcgcg gcgtcggcat ggtgcaggcg ttcaccctgg tgaagaacaa ggcgaagcgc
     1381 gagctgttcc ccgatttcgg cgagatcggc acgctgtgcc gcgacatctt cttccgcaac
     1441 aacctgatca tgcgggcatg cggcgaccac atcgtgtcgg cgccgccgct ggtgatgacg
     1501 cgggcggaag tggacgagat gctggcggtg gcggaacgct gtctggagga attcgagcag
     1561 acgctgaagg cgcgcgggct ggcttaagct tgatccggct gctaacaaag cccgaaagga
     1621 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     1681 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatctg gcgtaatagc
     1741 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     1801 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1861 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1921 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     1981 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2041 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2101 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2161 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2221 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     2281 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2341 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2401 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2461 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2521 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2581 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     2641 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2701 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2761 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2821 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2881 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2941 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3001 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3061 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3121 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3181 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3241 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3301 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3361 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3421 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3541 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4021 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4081 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4141 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4201 aaccgcctct ccccgcgcgt tggccgattc attaatgcag
//
