LOCUS       pAAV-Ef1a-FAS-NE        6843 bp DNA     circular SYN 02-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 2, 2024 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Sequence Label: pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE
FEATURES             Location/Qualifiers
     source          1..6843
                     /note="/dnas_title=pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE-pA with
                     cloning primers"
                     /organism="synthetic DNA construct"
     repeat_region   12..141
                     /label=AAV2ITR
     promoter        249..1419
                     /label=Ef1a promotor
                     /note="Ef1a promotor"
     primer_bind     1367..1387
                     /label=#25_Ef1a-Fwd
     misc_feature    1411..3019
                     /label=Seq conf 04Oct22
     repeat_region   1450..1483
                     /label=loxFAS
                     /note="loxFAS"
     primer_bind     1471..1509
                     /label=#12
     misc_feature    1492..1551
                     /label=NES
                     /note="NES"
     CDS             1552..2904
                     /codon_start=1
                     /label=R-GECO1 gene cds
                     /note="R-GECO1 gene cds"
                     /translation="GSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNKA
                     GHAVRAIGRLSSPVVSERMYPEDGALKSEIKKGLRLKDGGHYAAEVKTTYKAKKPVQLP
                     GAYIVDIKLDIVSHNEDYTIVEQCERAEGRHSTGGMDELYKGGTGGSLVSKGEEDNMAI
                     IKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLPFAWDILSPQFM
                     YGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSLQDGVFIYKVKLR
                     GTNFPPDGPVMQKKTMGWEATRDDLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVFRS
                     LGQNPTEAELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGN
                     GYIGAAELRHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     misc_feature    1558..1575
                     /label=HIS Tag
                     /note="HIS Tag"
     misc_feature    1579..1611
                     /label=T7 leader
                     /note="T7 leader"
     primer_bind     1699..1716
                     /label=#15
     misc_feature    2008..2451
                     /label=mCherry
                     /note="mCherry"
     primer_bind     2093..2109
                     /label=#16
     primer_bind     2470..2486
                     /label=#14
     primer_bind     complement(2884..2921)
                     /label=#13
     repeat_region   2912..2945
                     /label=loxFAS
                     /note="loxFAS"
     regulatory      2970..3558
                     /label=WPRE
                     /regulatory_class="transcriptional_cis_regulatory_region"
     primer_bind     complement(3039..3055)
                     /label=WPRE-84R
     polyA_signal    3590..4066
                     /label=hGHpA
     repeat_region   4106..4235
                     /label=AAV2ITR
     misc_feature    5163..6023
                     /label=Ampicillin
                     /note="Ampicillin"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc
     1441 gactccggaa caacttcgta tatacctttc tatacgaagt tgtggcgcgc catgctgcag
     1501 aacgagcttg ctcttaagtt ggctggactt gatattaaca agactggagg aggttctcat
     1561 catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg
     1621 tacgacgatg acgataagga tctcgcaaca atggtcgact catcacgtcg taagtggaat
     1681 aaggcaggtc acgcagtcag agctataggt cggctgagct cacccgtggt ttccgagcgg
     1741 atgtaccccg aggacggcgc cctgaagagc gagatcaaga aggggctgag gctgaaggac
     1801 ggcggccact acgccgccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     1861 cccggcgcct acatcgtgga catcaagttg gacatcgtgt cccacaacga ggactacacc
     1921 atcgtggaac agtgcgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     1981 tacaagggag gtacaggcgg gagtctggtg agcaagggcg aggaggataa catggccatc
     2041 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     2101 gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc taagctgaag
     2161 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     2221 ggctccaagg cctacattaa gcacccagcc gacatccccg actacttcaa gctgtccttc
     2281 cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat tattcacgtg
     2341 aaccaggact cctccctgca ggacggcgta ttcatctaca aggtgaagct gcgcggcacc
     2401 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggctacgcgt
     2461 gacgacctga ctgaagagca gatcgcagaa tttaaagagg ctttctccct atttgacaag
     2521 gacggggatg ggacgataac aaccaaggag cttgggacgg tgttccgatc gctggggcag
     2581 aaccccacag aagcagagct gcaggacatg atcaatgaag tagatgccga cggtgacggc
     2641 acattcgact tccctgagtt cctgacgatg atggcaagaa aaatgaatga cacagacagt
     2701 gaagaggaaa ttcgcgaagc gttccgcgtg tttgataagg acggcaatgg ctacatcggc
     2761 gcagcagagc ttcgccacgt gatgacagac cttggagaga agttaacaga tgaggaggtt
     2821 gatgaaatga tcagggtagc agacatcgat ggggatggtc aggtaaacta cgaagagttt
     2881 gtacaaatga tgacagcgaa gtagggctag cacaacttcg tatatacctt tctatacgaa
     2941 gttgtgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     3001 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3061 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3121 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3181 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3241 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3301 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3361 ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga
     3421 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3481 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3541 tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc
     3601 ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag
     3661 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat
     3721 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc
     3781 ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat
     3841 ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca
     3901 ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat
     3961 attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa
     4021 attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt
     4081 aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
     4141 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     4201 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
     4261 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     4321 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4381 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4441 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4501 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     4561 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4621 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     4681 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4741 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     4801 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     4861 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     4921 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     4981 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5041 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5101 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5161 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5221 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5281 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5341 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5401 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5461 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5521 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5581 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5641 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5701 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5761 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5821 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5881 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5941 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6001 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6061 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6121 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6181 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6241 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6301 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     6361 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6421 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6481 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6541 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6601 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6661 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6721 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6781 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6841 tgt
//
