LOCUS       pAAV-Ef1a-FAS-GC        6786 bp    DNA     linear       19-JUL-2019
FEATURES             Location/Qualifiers
     misc_feature    249..1419
                     /note="EF1a_promoter"
     misc_feature    1367..1387
                     /note="EF1a_fwd_primer"
     misc_feature    2915..3502
                     /note="WPRE"
     misc_feature    3531..4009
                     /note="hGH_PA_terminator"
     misc_feature    4281..4587
                     /note="f1_origin"
     misc_feature    4855..4877
                     /note="pGEX_3_primer"
     misc_feature    5036..5064
                     /note="AmpR_promoter"
     misc_feature    5106..5966
                     /note="Ampicillin"
     misc_feature    6121..6740
                     /note="pBR322_origin"
     misc_feature    1450..1483
                     /note="loxFAS"
     misc_feature    1492..2847
                     /note="GCaMP6f"
     PCR_primer      1471..1510
                     /pair=""
                     /primer="TATACGAAGTTGTGGCGCGCCATGGGTTCTCATCATCATC"
                     /current=0
     misc_feature    2855..2888
                     /note="loxFAS"
     PCR_primer      complement(2829..2868)
                     /pair=""
                     /primer="ATATACGAAGTTGTGCTAGCCGTTATCCTTCGCTGTCATC"
                     /current=0
     PCR_primer      complement(2982..2998)
                     /pair=""
                     /primer="GCAGCGTATCCACATAG"
                     /current=0
     PCR_primer      1879..1897
                     /pair=""
                     /primer="ATGGTCCTGCTGGAGTTCG"
                     /current=1
     misc_feature    1492..2842
                     /note="GCaMP6f sequence confirmed"
     misc_feature    2440..2442
                     /note=""
     misc_feature    1723..1725
                     /note=""
     source          1..6786
                     /dnas_title="pAAV-Ef1a-FAS-GCaMP6f-WPRE-pA_Karen
                     completed"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccGGATC CTCTAGAGTC
     1441 GACTCCGGAA CAACTTCGTA TATACCTTTC TATACGAAGT TGTGGCGCGC Catgggttct
     1501 catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat
     1561 ctgtacgacg atgacgataa ggatctcgcc accatggtcg actcatcacg tcgtaagtgg
     1621 aataagacag gtcacgcagt cagagctata ggtcggctga gctcactcga gaacgtctat
     1681 atcaaggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
     1741 gaggacggcg gcgtgcagct cgcctaccac taccagcaga acacccccat cggcgacggc
     1801 cccgtgctgc tgcccgacaa ccactacctg agcgtgcagt ccaaactttc gaaagacccc
     1861 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1921 ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagcaa gggcgaggag
     1981 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2041 ttcagcgtgt ccggcgaggg tgagggcgat gccacctacg gcaagctgac cctgaagttc
     2101 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     2161 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2221 gccatgcccg aaggctacat ccaggagcgc accatcttct tcaaggacga cggcaactac
     2281 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2341 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacctgccg
     2401 gaccaactga ctgaagagca gatcgcagaa tttaaagagg aattctccct atttgacaag
     2461 gacggggatg ggacaataac aaccaaggag ctggggacgg tgatgcggtc tctggggcag
     2521 aaccccacag aagcagagct gcaggacatg atcaatgaag tagatgccga cggtgacggc
     2581 acaatcgact tccctgagtt cctgacaatg atggcaagaa aaatgaaata cagggacacg
     2641 gaagaagaaa ttagagaagc gttcggtgtg tttgataagg atggcaatgg ctacatcagt
     2701 gcagcagagc ttcgccacgt gatgacaaac cttggagaga agttaacaga tgaagaggtt
     2761 gatgaaatga tcagggaagc agacatcgat ggggatggtc aggtaaacta cgaagagttt
     2821 gtacaaatga tgacagcgaa ggataacGGC TAGCACAACT TCGTATATAC CTTTCTATAC
     2881 GAAGTTGTGA ATTCgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     2941 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3001 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     3061 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     3121 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     3181 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     3241 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     3301 cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg
     3361 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     3421 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     3481 ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca
     3541 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
     3601 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
     3661 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
     3721 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
     3781 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
     3841 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
     3901 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
     3961 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta
     4021 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     4081 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     4141 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     4201 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     4261 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4321 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4381 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4441 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     4501 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4561 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     4621 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4681 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     4741 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     4801 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4861 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     4921 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4981 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5041 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5101 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5161 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5221 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5281 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5341 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5401 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5461 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5521 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5581 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5641 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5701 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5761 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5821 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5881 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5941 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6001 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6061 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6121 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6181 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6241 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     6301 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6361 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6421 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6481 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6541 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6601 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6661 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6721 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6781 acatgt
//

