LOCUS Exported 10789 bp ds-DNA circular SYN 04-MAR-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10789) AUTHORS DD TITLE Direct Submission JOURNAL Exported Friday, Mar 20, 2020 from SnapGene Viewer 5.0.7 https://www.snapgene.com FEATURES Location/Qualifiers source 1..10789 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 32..892 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1063..1651 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2013..2029 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2037..2053 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2074..2092 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 2113..2554 /gene="S. cerevisiae GAL1" /label=GAL1 promoter /note="inducible promoter, regulated by Gal4" protein_bind 2113..2230 /label=UAS /bound_moiety="Gal4" /note="upstream activating sequence mediating Gal4-dependent induction" CDS 2582..6685 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 6698..6718 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 6734..6981 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" promoter 7052..7332 /label=TRP1 promoter CDS 7333..8007 /codon_start=1 /label=TRP1 /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA KK" rep_origin 8364..9706 /label=2u ori /note="yeast 2u plasmid origin of replication" protein_bind complement(9327..9374) /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." promoter 9767..10035 /label=SNR52 promoter misc_feature 10036..10073 /label=stuffer CDS 10074..10152 /codon_start=1 /label=gRNA scaffold /translation="VLELEIAS*NKASPLST*KSGTESVV" misc_feature 10153..10172 /label=SUP4 terminator promoter 10243..10511 /label=SNR52 promoter misc_feature 10512..10549 /label=stuffer CDS 10550..10628 /codon_start=1 /label=gRNA scaffold /translation="VLELEIAS*NKASPLST*KSGTESVV" misc_feature 10629..10648 /label=SUP4 terminator promoter join(10716..10789,1..31) /gene="bla" /label=AmpR promoter ORIGIN 1 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 61 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 121 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 181 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 241 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 301 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 361 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 421 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 481 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 541 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 601 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 661 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 721 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 781 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 841 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 901 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 961 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1021 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1081 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1141 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1201 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 1261 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 1321 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 1381 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 1441 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 1501 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 1561 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 1621 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 1681 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 1741 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 1801 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 1861 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1921 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 1981 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 2041 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 2101 gctggagctg tacggattag aagccgccga gcgggtgaca gccctccgaa ggaagactct 2161 cctccgtgcg tcctcgtctt caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg 2221 cactgctccg aacaataaag attctacaat actagctttt atggttatga agaggaaaaa 2281 ttggcagtaa cctggcccca caaaccttca aatgaacgaa tcaaattaac aaccatagga 2341 tgataatgcg attagttttt tagccttatt tctggggtaa ttaatcagcg aagcgatgat 2401 ttttgatcta ttaacagata tataaatgca aaaactgcat aaccacttta actaatactt 2461 tcaacatttt cggtttgtat tacttcttat tcaaatgtaa taaaagtatc aacaaaaaat 2521 tgttaatata cctctatact ttaacgtcaa ggagaaaaaa ccccgggatc ccccgggaaa 2581 aatggacaag aagtactcca ttgggctcga tatcggcaca aacagcgtcg gttgggccgt 2641 cattacggac gagtacaagg tgccgagcaa aaaattcaaa gttctgggca ataccgatcg 2701 ccacagcata aagaagaacc tcattggcgc cctcctgttc gactccgggg agacggccga 2761 agccacgcgg ctcaaaagaa cagcacggcg cagatatacc cgcagaaaga atcggatctg 2821 ctacctgcag gagatcttta gtaatgagat ggctaaggtg gatgactctt tcttccatag 2881 gctggaggag tcctttttgg tggaggagga taaaaagcac gagcgccacc caatctttgg 2941 caatatcgtg gacgaggtgg cgtaccatga aaagtaccca accatatatc atctgaggaa 3001 gaagcttgta gacagtactg ataaggctga cttgcggttg atctatctcg cgctggcgca 3061 tatgatcaaa tttcggggac acttcctcat cgagggggac ctgaacccag acaacagcga 3121 tgtcgacaaa ctctttatcc aactggttca gacttacaat cagcttttcg aagagaaccc 3181 gatcaacgca tccggagttg acgccaaagc aatcctgagc gctaggctgt ccaaatcccg 3241 gcggctcgaa aacctcatcg cacagctccc tggggagaag aagaacggcc tgtttggtaa 3301 tcttatcgcc ctgtcactcg ggctgacccc caactttaaa tctaacttcg acctggccga 3361 agatgccaag cttcaactga gcaaagacac ctacgatgat gatctcgaca atctgctggc 3421 ccagatcggc gaccagtacg cagacctttt tttggcggca aagaacctgt cagacgccat 3481 tctgctgagt gatattctgc gagtgaacac ggagatcacc aaagctccgc tgagcgctag 3541 tatgatcaag cgctatgatg agcaccacca agacttgact ttgctgaagg cccttgtcag 3601 acagcaactg cctgagaagt acaaggaaat tttcttcgat cagtctaaaa atggctacgc 3661 cggatacatt gacggcggag caagccagga ggaattttac aaatttatta agcccatctt 3721 ggaaaaaatg gacggcaccg aggagctgct ggtaaagctt aacagagaag atctgttgcg 3781 caaacagcgc actttcgaca atggaagcat cccccaccag attcacctgg gcgaactgca 3841 cgctatcctc aggcggcaag aggatttcta cccctttttg aaagataaca gggaaaagat 3901 tgagaaaatc ctcacatttc ggatacccta ctatgtaggc cccctcgccc ggggaaattc 3961 cagattcgcg tggatgactc gcaaatcaga agagaccatc actccctgga acttcgagga 4021 agtcgtggat aagggggcct ctgcccagtc cttcatcgaa aggatgacta actttgataa 4081 aaatctgcct aacgaaaagg tgcttcctaa acactctctg ctgtacgagt acttcacagt 4141 ttataacgag ctcaccaagg tcaaatacgt cacagaaggg atgagaaagc cagcattcct 4201 gtctggagag cagaagaaag ctatcgtgga cctcctcttc aagacgaacc ggaaagttac 4261 cgtgaaacag ctcaaagaag actatttcaa aaagattgaa tgtttcgact ctgttgaaat 4321 cagcggagtg gaggatcgct tcaacgcatc cctgggaacg tatcacgatc tcctgaaaat 4381 cattaaagac aaggacttcc tggacaatga ggagaacgag gacattcttg aggacattgt 4441 cctcaccctt acgttgtttg aagataggga gatgattgaa gaacgcttga aaacttacgc 4501 tcatctcttc gacgacaaag tcatgaaaca gctcaagagg cgccgatata caggatgggg 4561 gcggctgtca agaaaactga tcaatgggat ccgagacaag cagagtggaa agacaatcct 4621 ggattttctt aagtccgatg gatttgccaa ccggaacttc atgcagttga tccatgatga 4681 ctctctcacc tttaaggagg acatccagaa agcacaagtt tctggccagg gggacagtct 4741 tcacgagcac atcgctaatc ttgcaggtag cccagctatc aaaaagggaa tactgcagac 4801 cgttaaggtc gtggatgaac tcgtcaaagt aatgggaagg cataagcccg agaatatcgt 4861 tatcgagatg gcccgagaga accaaactac ccagaaggga cagaagaaca gtagggaaag 4921 gatgaagagg attgaagagg gtataaaaga actggggtcc caaatcctta aggaacaccc 4981 agttgaaaac acccagcttc agaatgagaa gctctacctg tactacctgc agaacggcag 5041 ggacatgtac gtggatcagg aactggacat caatcggctc tccgactacg acgtggatca 5101 tatcgtgccc cagtcttttc tcaaagatga ttctattgat aataaagtgt tgacaagatc 5161 cgataaaaat agagggaaga gtgataacgt cccctcagaa gaagttgtca agaaaatgaa 5221 aaattattgg cggcagctgc tgaacgccaa actgatcaca caacggaagt tcgataatct 5281 gactaaggct gaacgaggtg gcctgtctga gttggataaa gccggcttca tcaaaaggca 5341 gcttgttgag acacgccaga tcaccaagca cgtggcccaa attctcgatt cacgcatgaa 5401 caccaagtac gatgaaaatg acaaactgat tcgagaggtg aaagttatta ctctgaagtc 5461 taagctggtc tcagatttca gaaaggactt tcagttttat aaggtgagag agatcaacaa 5521 ttaccaccat gcgcatgatg cctacctgaa tgcagtggta ggcactgcac ttatcaaaaa 5581 atatcccaag cttgaatctg aatttgttta cggagactat aaagtgtacg atgttaggaa 5641 aatgatcgca aagtctgagc aggaaatagg caaggccacc gctaagtact tcttttacag 5701 caatattatg aattttttca agaccgagat tacactggcc aatggagaga ttcggaagcg 5761 accacttatc gaaacaaacg gagaaacagg agaaatcgtg tgggacaagg gtagggattt 5821 cgcgacagtc cggaaggtcc tgtccatgcc gcaggtgaac atcgttaaaa agaccgaagt 5881 acagaccgga ggcttctcca aggaaagtat cctcccgaaa aggaacagcg acaagctgat 5941 cgcacgcaaa aaagattggg accccaagaa atacggcgga ttcgattctc ctacagtcgc 6001 ttacagtgta ctggttgtgg ccaaagtgga gaaagggaag tctaaaaaac tcaaaagcgt 6061 caaggaactg ctgggcatca caatcatgga gcgatcaagc ttcgaaaaaa accccatcga 6121 ctttctcgag gcgaaaggat ataaagaggt caaaaaagac ctcatcatta agcttcccaa 6181 gtactctctc tttgagcttg aaaacggccg gaaacgaatg ctcgctagtg cgggcgagct 6241 gcagaaaggt aacgagctgg cactgccctc taaatacgtt aatttcttgt atctggccag 6301 ccactatgaa aagctcaaag ggtctcccga agataatgag cagaagcagc tgttcgtgga 6361 acaacacaaa cactaccttg atgagatcat cgagcaaata agcgaattct ccaaaagagt 6421 gatcctcgcc gacgctaacc tcgataaggt gctttctgct tacaataagc acagggataa 6481 gcccatcagg gagcaggcag aaaacattat ccacttgttt actctgacca acttgggcgc 6541 gcctgcagcc ttcaagtact tcgacaccac catagacaga aagcggtaca cctctacaaa 6601 ggaggtcctg gacgccacac tgattcatca gtcaattacg gggctctatg aaacaagaat 6661 cgacctctct cagctcggtg gagacagcag ggctgacccc aagaagaaga ggaaggtgtg 6721 atctcttctc gagtcatgta attagttatg tcacgcttac attcacgccc tccccccaca 6781 tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt 6841 tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg 6901 tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga 6961 cgctcgaagg ctttaatttg cggaggcgta tcacgaggcc ctttcgtctt caagaattag 7021 cttttcaagt actgagagtg caccatagat cacgacatta ctatatatat aatataggaa 7081 gcatttaata gaacagcatc gtaatatatg tgtactttgc agttatgacg ccagatggca 7141 gtagtggaag atattcttta ttgaaaaata gcttgtcacc ttacgtacaa tcttgatccg 7201 gagcttttct ttttttgccg attaagaatt aattcggtcg aaaaaagaaa aggagagggc 7261 caagagggag ggcattggtg actattgagc acgtgagtat acgtgattaa gcacacaaag 7321 gcagcttgga gtatgtctgt tattaatttc acaggtagtt ctggtccatt ggtgaaagtt 7381 tgcggcttgc agagcacaga ggccgcagaa tgtgctctag attccgatgc tgacttgctg 7441 ggtattatat gtgtgcccaa tagaaagaga acaattgacc cggttattgc aaggaaaatt 7501 tcaagtcttg taaaagcata taaaaatagt tcaggcactc cgaaatactt ggttggcgtg 7561 tttcgtaatc aacctaagga ggatgttttg gctctggtca atgattacgg cattgatatc 7621 gtccaactgc atggagatga gtcgtggcaa gaataccaag agttcctcgg tttgccagtt 7681 attaaaagac tcgtatttcc aaaagactgc aacatactac tcagtgcagc ttcacagaaa 7741 cctcattcgt ttattccctt gtttgattca gaagcaggtg ggacaggtga acttttggat 7801 tggaactcga tttctgactg ggttggaagg caagagagcc ccgaaagctt acattttatg 7861 ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc ttagattaaa tggcgttatt 7921 ggtgttgatg taagcggagg tgtggagaca aatggtgtaa aagactctaa caaaatagca 7981 aatttcgtca aaaatgctaa gaaataggtt attactgagt agtataaaac tgtattataa 8041 gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata tcagttatta 8101 cccaattctc atgtttgaca gcttatcatc gatcgtccaa ctgcatggag atgagtcgtg 8161 gcaagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat ttccaaaaga 8221 ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc ccttgtttga 8281 ttcagaagca ggtgggacag gtgaactttt ggattggaac tcgatttctg actgggttgg 8341 aaggcaagag agccccgagc ctgaacgaag catctgtgct tcattttgta gaacaaaaat 8401 gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga 8461 aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca 8521 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga 8581 acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt 8641 ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttagg ttactttttt 8701 tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta 8761 aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca 8821 cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat 8881 ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc 8941 gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat 9001 actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc 9061 ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt 9121 cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag 9181 cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa 9241 tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga 9301 gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc 9361 ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acacgagctg 9421 cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata 9481 tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat 9541 ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc cacgcggggt 9601 atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg 9661 gattagtctc atccttcaat gctatcattt cctttgatat tggatcatac agcatgtgac 9721 cttttgcaag caattaaatt ttgctgatat cgaattcgct ggagcttctt tgaaaagata 9781 atgtatgatt atgctttcac tcatatttat acagaaactt gatgttttct ttcgagtata 9841 tacaaggtga ttacatgtac gtttgaagta caactctaga ttttgtagtg ccctcttggg 9901 ctagcggtaa aggtgcgcat tttttcacac cctacaatgt tctgttcaaa agattttggt 9961 caaacgctgt agaagtgaaa gttggtgcgc atgtttcggc gttcgaaact tctccgcagt 10021 gaaagataaa tgatcgttta aacgacgaca tcagcaacat ctgcgagggt accgttttag 10081 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg 10141 agtcggtggt gctttttttg ttttttatgt cttcgagtca tgtaattagt tatgtcacgc 10201 tctagagcgg ccgccaccgc tgatatcgaa ttcgctggag cttctttgaa aagataatgt 10261 atgattatgc tttcactcat atttatacag aaacttgatg ttttctttcg agtatataca 10321 aggtgattac atgtacgttt gaagtacaac tctagatttt gtagtgccct cttgggctag 10381 cggtaaaggt gcgcattttt tcacacccta caatgttctg ttcaaaagat tttggtcaaa 10441 cgctgtagaa gtgaaagttg gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa 10501 gataaatgat cgtttaaacg acgacatcag caacatctgc gagggtaccg ttttagagct 10561 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc 10621 ggtggtgctt tttttgtttt ttatgtcttc gagtcatgta acaccgcggt ggagctccag 10681 cttttgttcc cggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 10741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgata //