LOCUS       Exported               13299 bp ds-DNA     circular SYN 25-MAR-2020
DEFINITION  Gateway cloning compatible binary vector for expression of gene by 
            CaMV35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pGWB402.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13299)
  AUTHORS   Nakagawa, T, Suzuki, T, Murata, S, Nakamura, S, Hino, T, Maeo, K, 
            Tabata, R, Kawai, T, Tanaka, K, Niwa, Y, Watanabe, Y, Nakamura, K, 
            Kimura, T, Ishiguro, S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 13299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 27, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13299
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    301..306
                     /label=HindIII
                     /note="Geneious type: motif"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower
                     mosaic virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    1166..1171
                     /label=XbaI
                     /note="Geneious type: motif"
     misc_feature    1172..4523
                     /label=GTTATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATG
                     /label=GTTATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATA 
                     TAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA 
                     AACACAACATATCCAGTCACTATGGCGGCCCCTCGAGGGGCCGCATTAGGCACCCCAG 
                     GCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGC 
                     GAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACC 
                     GTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTC 
                     AATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAA 
                     GAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAAT 
                     GCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTG 
                     TTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAG 
                     TGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGT 
                     TACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCT 
                     CAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAA 
                     CTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTG 
                     ATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAA 
                     TGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGG 
                     ATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGT 
                     ATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAA 
                     GCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATG 
                     ATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCG 
                     TGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATT 
                     GAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGTT 
                     TACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTA 
                     TTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGA 
                     TAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATG 
                     ATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATC 
                     TCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATA 
                     AATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATG 
                     TTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTG 
                     ATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGATAACA 
                     GCGCTT
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1172..1204
                     /label=SV40 large T antigen NLS
     misc_feature    1205..1210
                     /label=SpeI
     misc_feature    1211..1336
                     /label=attR1
                     /note="Geneious type: motif"
     misc_feature    1456..2113
                     /label=CmR
                     /note="Geneious type: motif"
     misc_feature    2457..2759
                     /label=ccdB
                     /note="Geneious type: motif"
     misc_feature    complement(2804..2927)
                     /label=attR2
                     /note="Geneious type: motif"
     misc_feature    2928..2933
                     /label=ApaI
                     /note="Geneious type: motif"
     misc_signal     2935..3630
                     /label=mScarlet-I
     misc_feature    3631..3636
                     /label=MluI
                     /note="Geneious type: motif"
     misc_feature    3637..3792
                     /label=*F2A
                     /note="Geneious type: motif"
     misc_feature    3793..3798
                     /label=KpnI
                     /note="Geneious type: motif"
     CDS             3799..4521
                     /label=VENUS
     misc_feature    4525..4530
                     /label=SacI
                     /note="Geneious type: motif"
     terminator      4541..4793
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4827..4843)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4827..4843)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4840..4862)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4851..4867
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4875..4905)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4920..4941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      4975..5227
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5617..6411)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5728..5747)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6338..6357
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6432..6615)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(6468..6647)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6821..6845
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7366..8157
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8403..8991
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8892..8911
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9145..9162
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9177..9317
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9243..9262)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     9403..9425
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9661..9855
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9921..10994)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11423..12052)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgccaaaa aagaagagaa aggtagaaga
     1201 ccccactagt acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     1261 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     1321 tatccagtca ctatggcggc ccctcgaggg gccgcattag gcaccccagg ctttacactt
     1381 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccggcgag attttcagga
     1441 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     1501 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     1561 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     1621 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     1681 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     1741 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     1801 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     1861 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     1921 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     1981 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc
     2041 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     2101 cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca gtatgcgtat
     2161 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     2221 aaaagaggtg tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     2281 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     2341 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     2401 cgcccggttt attgaaatga acggctcttt tgctgacgag aacagggact ggtgaaatgc
     2461 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     2521 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     2581 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     2641 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     2701 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     2761 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     2821 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     2881 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtggg cccaatggtg
     2941 tccaagggcg aagctgtgat taaagaattt atgcgtttta aggtccacat ggaaggatcc
     3001 atgaatggac atgaattcga aatcgagggg gagggcgagg gccgacctta tgagggtaca
     3061 cagaccgcga agctaaaggt aaccaaggga ggcccactac cctttagttg ggacatactc
     3121 agcccgcagt ttatgtatgg cagtcgtgcc tttataaagc acccagcgga tataccagac
     3181 tactacaaac agtccttccc agaggggttt aaatgggagc gagtgatgaa ctttgaggac
     3241 ggtggtgctg tgacggtaac tcaggacaca tcactcgaag acgggactct tatttacaaa
     3301 gtaaaactga gagggaccaa ttttccacca gatggccccg tcatgcaaaa aaagacgatg
     3361 ggttgggaag catctacaga acgactctat cccgaggacg gcgtcctcaa aggcgacatt
     3421 aaaatggcct tgaggttgaa agacggtgga cgttacttgg cggacttcaa aacaacttat
     3481 aaagcaaaaa agccagtgca aatgcctgga gcgtacaatg tagatagaaa acttgatata
     3541 acgtcacata atgaggacta taccgtggtt gagcaatacg agaggtcaga aggacgacac
     3601 agcaccggcg gtatggacga gttgtacaag acgcgttcta atgccgccga tgaggttgcg
     3661 actggatcag gtcttttagc gatacatccg accgaagcgc gacataaaca aaaaattgta
     3721 gcgccggtca aacaaaccct gaatttcgac ttactcaaac ttgcgggtga cgtagagtca
     3781 aatccagggc ctggtaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3841 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     3901 gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg
     3961 cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc
     4021 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     4081 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4141 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4201 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc
     4261 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
     4321 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     4381 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     4441 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     4501 gacgaggcag ctagatccac ctaagagctc gaatttcccc gatcgttcaa acatttggca
     4561 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     4621 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     4681 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     4741 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     4801 ggttccggaa ccaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     4861 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     4921 tgagtgagct aactcacatt aggctgaatt aggcgcgcct atttctgaat tcccgatcta
     4981 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
     5041 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
     5101 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
     5161 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
     5221 aacgatcggg gatcatccgg gtctgtggcg ggaactccac gaaaatatcc gaacgcagca
     5281 agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg ttgatgtgga
     5341 cgccgggccc gatcatattg tcgctcagga tcgtggcgtt gtgcttgtcg gccgttgctg
     5401 tcgtaatgat atcggcacct tcgaccgcct gttccgcaga gatcccgtgg gcgaagaact
     5461 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     5521 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg
     5581 tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     5641 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     5701 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     5761 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     5821 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcatcgc cgtcgggcat
     5881 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     5941 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     6001 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     6061 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     6121 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     6181 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     6241 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     6301 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     6361 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     6421 tccagatccg gtgcagatta tttggattga gagtgaatat gagactctaa ttggataccg
     6481 aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc tagctgatag
     6541 tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc ttagctcatt
     6601 aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt cagttccaaa
     6661 cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc ttaattctcc
     6721 gctcatgatg aattcagaaa taaattcagc ctaattcggc gttaattcag tacattaaaa
     6781 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     6841 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     6901 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     6961 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     7021 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     7081 tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     7141 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     7201 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     7261 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     7321 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
     7381 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     7441 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     7501 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     7561 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     7621 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     7681 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     7741 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     7801 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     7861 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     7921 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     7981 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     8041 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     8101 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     8161 ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     8221 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     8281 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc
     8341 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8401 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8461 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8521 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     8581 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8641 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8701 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8761 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8821 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8881 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8941 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9001 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9061 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9121 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     9181 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     9241 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     9301 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     9361 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     9421 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
     9481 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
     9541 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
     9601 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
     9661 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
     9721 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
     9781 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg
     9841 tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc
     9901 cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg
     9961 tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc
    10021 aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg
    10081 ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg
    10141 cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg
    10201 tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct
    10261 agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta
    10321 atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc
    10381 tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc
    10441 tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg
    10501 cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa
    10561 accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa
    10621 acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc
    10681 ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca
    10741 tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac
    10801 ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc
    10861 cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc
    10921 ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt
    10981 ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc
    11041 agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca
    11101 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc
    11161 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc
    11221 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc
    11281 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt
    11341 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc
    11401 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct
    11461 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg
    11521 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc
    11581 cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga
    11641 caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca
    11701 ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg
    11761 ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga
    11821 agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa
    11881 cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg
    11941 aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga
    12001 tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc
    12061 cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct
    12121 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
    12181 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
    12241 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
    12301 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
    12361 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
    12421 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
    12481 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
    12541 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
    12601 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
    12661 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
    12721 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
    12781 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
    12841 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
    12901 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
    12961 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
    13021 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
    13081 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
    13141 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg
    13201 actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt
    13261 ggttggcatg cacatacaaa tggacgaacg gataaacct
//
