LOCUS       Exported               14044 bp ds-DNA     circular SYN 25-MAR-2020
DEFINITION  Gateway cloning compatible binary vector for simple cloning of gene.
ACCESSION   .
VERSION     .
KEYWORDS    pGWB401.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Nakagawa, T, Suzuki, T, Murata, S, Nakamura, S, Hino, T, Maeo, K, 
            Tabata, R, Kawai, T, Tanaka, K, Niwa, Y, Watanabe, Y, Nakamura, K, 
            Kimura, T, Ishiguro, S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 14044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 27, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14044
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
                     /note="color: #ffffff"
     source          4382..4537
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
                     /note="color: #ffffff"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #a6acb3"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    301..306
                     /label=HindIII
                     /note="Geneious type: motif"
                     /note="color: #a6acb3"
     misc_feature    307..5268
                     /label=GCATGCCTGCAGGTCGACTCTAGAGTTATCAACAAGTTTGTACAAAA
                     /label=GCATGCCTGCAGGTCGACTCTAGAGTTATCAACAAGTTTGTACAAAAAAG 
                     CTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAA 
                     AAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCCCTC 
                     GAGGGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGT 
                     GTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAG 
                     AAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATT 
                     TTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATAT 
                     TACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATT 
                     CACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACG 
                     GTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAAC 
                     TGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACAC 
                     ATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGT 
                     TTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGA 
                     TTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATAT 
                     TATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCT 
                     GTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTG 
                     GCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCG 
                     TATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTA 
                     TGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGC 
                     TATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCAT 
                     GCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGG 
                     ATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGG 
                     ACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGT 
                     TTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCT 
                     GGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCAT 
                     ATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCG 
                     TTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCAT 
                     TAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCA 
                     GGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTA 
                     TGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTT 
                     TCTTGTACAAAGTGGTTGATAACAGCGCTT
                     /note="Geneious type: Editing History Replacement"
                     /note="color: #a6acb3"
     promoter        307..1904
                     /label=UBQ10
                     
                     /note="color: #ffffff"
     misc_feature    1911..1916
                     /label=XbaI
                     /note="Geneious type: motif"
                     /note="color: #a6acb3"
     misc_feature    1917..1949
                     /label=SV40 large T antigen NLS
                     /note="color: #a6acb3"
     misc_feature    1950..1955
                     /label=SpeI
                     /note="color: #a6acb3"
     misc_feature    1956..2081
                     /label=attR1
                     /note="color: #a6acb3"
     misc_feature    2201..2857
                     /label=CmR
                     /note="color: #a6acb3"
     misc_feature    3202..3504
                     /label=ccdB
                     /note="color: #a6acb3"
     misc_feature    complement(3549..3672)
                     /label=attR2
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    3673..3678
                     /label=ApaI
                     /note="Geneious type: motif"
                     /note="color: #a6acb3"
     misc_signal     3680..4375
                     /label=mScarlet-I
                     /note="color: #a6acb3"
     misc_signal     4376..4381
                     /label=MluI
                     /note="color: #a6acb3"
     misc_feature    4382..4537
                     /label=*F2A
                     /note="Geneious type: motif"
                     /note="color: #a6acb3"
     misc_feature    4538..4543
                     /label=KpnI
                     /note="Geneious type: motif"
                     /note="color: #a6acb3"
     CDS             4544..5266
                     /label=VENUS
                     /note="color: #993366; direction: RIGHT"
     misc_feature    5270..5275
                     /label=SacI
                     /note="Geneious type: motif"
                     /note="color: #a6acb3"
     terminator      5286..5538
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     primer_bind     complement(5572..5588)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(5572..5588)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(5585..5607)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    5596..5612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(5620..5650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    5665..5686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     terminator      5720..5972
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     CDS             complement(6362..7156)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6473..6492)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     7083..7102
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        complement(7177..7360)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(7213..7392)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    7566..7590
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #a6acb3"
     CDS             8111..8902
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /note="color: #ccffcc"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9148..9736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     9637..9656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     9890..9907
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    9922..10062
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     primer_bind     complement(9988..10007)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     10148..10170
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
                     /note="color: #a020f0; direction: RIGHT"
     rep_origin      10406..10600
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
     CDS             complement(10666..11739)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /note="color: #993366"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12168..12797)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagctttgac gtcgtatgat cgcgaagcac ccaccctaag ccacatttta atccttctgt
      361 tggccatgcc ccatcaaagt tgcacttaac ccaagattgt ggtggagctt cccatgtttc
      421 tcgtctgtcc cgacggtgtt gtggttggtg ctttccttac attctgagcc tctttccttc
      481 taatccactc atctgcatct tcttgtgtcc ttactaatac ctcattggtt ccaaattccc
      541 tccctttaag caccagctcg tttctgttct tccacagcct cccaagtatc caagggacta
      601 aagcctccac attcttcaga tcaggatatt cttgtttaag atgttgaact ctatggaggt
      661 ttgtatgaac tgatgatcta ggaccggata agttcccttc ttcatagcga acttattcaa
      721 agaatgtttt gtgtatcatt cttgttacat tgttattaat gaaaaaatat tattggtcat
      781 tggactgaac acgagtgtta aatatggacc aggccccaaa taagatccat tgatatatga
      841 attaaataac aagaataaat cgagtcacca aaccacttgc cttttttaac gagacttgtt
      901 caccaacttg atacaaaagt cattatccta tgcaaatcaa taatcataca aaaatatcca
      961 ataacactaa aaaattaaaa gaaatggata atttcacaat atgttatacg ataaagaagt
     1021 tacttttcca agaaattcac tgattttata agcccacttg cattagataa atggcaaaaa
     1081 aaaacaaaaa ggaaaagaaa taaagcacga agaattccag aaaatacgaa atacgcttca
     1141 atgcagtggg acccacggtt caattattgc caattttcag ctccaccgta tatttaaaaa
     1201 ataaaacgat aatgctaaaa aaatataaat cgtaacgatc gttaaatctc aacggctgga
     1261 tcttatgacg accgttagaa attgtggttg tcgacgagtc agtaataaac ggcgtcaaag
     1321 tggttgcagc cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc
     1381 aacctaaaaa taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac
     1441 gtgtcatttt attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc
     1501 tcgtcttttc ttcttcttct tctataaaac aatacccaaa gagttcttct tcttcacaat
     1561 tcagatttca atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa
     1621 ggtaaatttc tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca
     1681 atttcgtata tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg
     1741 gtttgatcgt tagatatcat cttaattctc gattagggtt tcatagatat catccgattt
     1801 gttcaaataa tttgagtttt gtcgaataat tactcttcga tttgtgattt ctatccagat
     1861 ctggtgttag tttctagttt gtgcgatcga atttgtcctc gagaggggac tctagaatgc
     1921 caaaaaagaa gagaaaggta gaagacccca ctagtacaag tttgtacaaa aaagctgaac
     1981 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
     2041 ctacataata ctgtaaaaca caacatatcc agtcactatg gcggcccctc gaggggccgc
     2101 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     2161 ttaggatccg gcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     2221 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     2281 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     2341 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     2401 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     2461 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     2521 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     2581 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     2641 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     2701 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     2761 ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga
     2821 attacaacag tactgcgatg agtggcaggg cggggcgtaa agatctggat ccggcttact
     2881 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     2941 tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg
     3001 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     3061 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     3121 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     3181 acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     3241 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
     3301 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     3361 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
     3421 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     3481 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
     3541 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     3601 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
     3661 gtacaaagtg gtgggcccaa tggtgtccaa gggcgaagct gtgattaaag aatttatgcg
     3721 ttttaaggtc cacatggaag gatccatgaa tggacatgaa ttcgaaatcg agggggaggg
     3781 cgagggccga ccttatgagg gtacacagac cgcgaagcta aaggtaacca agggaggccc
     3841 actacccttt agttgggaca tactcagccc gcagtttatg tatggcagtc gtgcctttat
     3901 aaagcaccca gcggatatac cagactacta caaacagtcc ttcccagagg ggtttaaatg
     3961 ggagcgagtg atgaactttg aggacggtgg tgctgtgacg gtaactcagg acacatcact
     4021 cgaagacggg actcttattt acaaagtaaa actgagaggg accaattttc caccagatgg
     4081 ccccgtcatg caaaaaaaga cgatgggttg ggaagcatct acagaacgac tctatcccga
     4141 ggacggcgtc ctcaaaggcg acattaaaat ggccttgagg ttgaaagacg gtggacgtta
     4201 cttggcggac ttcaaaacaa cttataaagc aaaaaagcca gtgcaaatgc ctggagcgta
     4261 caatgtagat agaaaacttg atataacgtc acataatgag gactataccg tggttgagca
     4321 atacgagagg tcagaaggac gacacagcac cggcggtatg gacgagttgt acaagacgcg
     4381 ttctaatgcc gccgatgagg ttgcgactgg atcaggtctt ttagcgatac atccgaccga
     4441 agcgcgacat aaacaaaaaa ttgtagcgcc ggtcaaacaa accctgaatt tcgacttact
     4501 caaacttgcg ggtgacgtag agtcaaatcc agggcctggt accatggtga gcaagggcga
     4561 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4621 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4681 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg
     4741 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4801 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4861 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4921 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4981 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     5041 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
     5101 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     5161 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     5221 cgccgccggg atcactctcg gcatggacga ggcagctaga tccacctaag agctcgaatt
     5281 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     5341 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     5401 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     5461 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     5521 catctatgtt actagatcgg gaattggttc cggaaccaat tcgtaatcat ggtcatagct
     5581 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5641 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaggct gaattaggcg
     5701 cgcctatttc tgaattcccg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     5761 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     5821 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     5881 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     5941 aagaaacttt attgccaaat gtttgaacga tcggggatca tccgggtctg tggcgggaac
     6001 tccacgaaaa tatccgaacg cagcaagata tcgcggtgca tctcggtctt gcctgggcag
     6061 tcgccgccga cgccgttgat gtggacgccg ggcccgatca tattgtcgct caggatcgtg
     6121 gcgttgtgct tgtcggccgt tgctgtcgta atgatatcgg caccttcgac cgcctgttcc
     6181 gcagagatcc cgtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag
     6241 ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg
     6301 aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg
     6361 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     6421 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     6481 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
     6541 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
     6601 cgacgagatc atcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
     6661 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
     6721 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
     6781 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
     6841 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
     6901 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
     6961 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
     7021 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
     7081 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
     7141 catcttgttc aatcatgcga aacgatccag atccggtgca gattatttgg attgagagtg
     7201 aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg
     7261 acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt
     7321 ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt ggctccttca
     7381 acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt
     7441 cataacgtga ctcccttaat tctccgctca tgatgaattc agaaataaat tcagcctaat
     7501 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     7561 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     7621 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     7681 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     7741 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     7801 gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg
     7861 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     7921 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     7981 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     8041 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     8101 gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     8161 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     8221 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     8281 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     8341 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     8401 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     8461 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     8521 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     8581 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     8641 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     8701 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     8761 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     8821 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     8881 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
     8941 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     9001 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     9061 agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     9121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     9181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     9241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     9301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     9421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     9481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     9541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9781 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9841 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9901 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     9961 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    10021 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
    10081 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
    10141 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
    10201 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
    10261 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
    10321 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
    10381 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
    10441 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
    10501 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
    10561 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
    10621 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
    10681 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
    10741 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
    10801 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
    10861 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
    10921 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
    10981 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
    11041 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
    11101 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
    11161 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
    11221 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
    11281 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
    11341 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
    11401 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
    11461 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
    11521 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
    11581 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
    11641 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
    11701 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
    11761 gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc
    11821 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
    11881 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
    11941 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
    12001 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
    12061 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
    12121 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
    12181 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
    12241 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
    12301 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
    12361 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
    12421 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
    12481 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
    12541 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
    12601 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
    12661 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
    12721 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
    12781 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
    12841 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
    12901 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
    12961 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
    13021 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
    13081 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
    13141 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
    13201 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
    13261 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
    13321 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
    13381 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
    13441 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    13501 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
    13561 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
    13621 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
    13681 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
    13741 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
    13801 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
    13861 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
    13921 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
    13981 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    14041 acct
//
