LOCUS       Exported                8983 bp ds-DNA     circular SYN 27-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 27, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    564..588
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             594..1838
                     /codon_start=1
                     /label=TARDBPG287A
                     /translation="MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRN
                     PVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTS
                     DLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMS
                     QRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFI
                     PKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPG
                     GFGNQAGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGML
                     ASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNG
                     GFGSSMDSKSSGWGML"
     protein_bind    complement(1840..1864)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1887..1934
                     /codon_start=1
                     /label=Beacon
                     /translation="ELFNLLGENQPPVVIK"
     CDS             1944..1967
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2004..2027
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2040..2057
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2067..2087
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2100..2120
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2133..2198
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2245..2362
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2411..2921
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             2943..3542
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             3670..3903
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3975..4096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4136..4271
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4292..4310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4320..4336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4478..4933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4959..5063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5064..5924
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6095..6683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6971..6992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7007..7037
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7045..7061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7069..7085
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7106..7124
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7152..7378
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7379..7559
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7606..7731
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8224..8457
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8642..8686
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaccgtcag atcgcctgga
      541 gacgccatcc acgctgtacg cgtacaagtt tgtacaaaaa agcaggctcc accatgtctg
      601 aatatattcg ggtaaccgaa gatgagaacg atgagcccat tgaaatacca tcggaagacg
      661 atgggacggt gctgctctcc acggttacag cccagtttcc aggggcgtgt gggcttcgct
      721 acaggaatcc agtgtctcag tgtatgagag gtgtccggct ggtagaagga attctgcatg
      781 ccccagatgc tggctgggga aatctggtgt atgttgtcaa ctatccaaaa gataacaaaa
      841 gaaaaatgga tgagacagat gcttcatcag cagtgaaagt gaaaagagca gtccagaaaa
      901 catccgattt aatagtgttg ggtctcccat ggaaaacaac cgaacaggac ctgaaagagt
      961 attttagtac ctttggagaa gttcttatgg tgcaggtcaa gaaagatctt aagactggtc
     1021 attcaaaggg gtttggcttt gttcgtttta cggaatatga aacacaagtg aaagtaatgt
     1081 cacagcgaca tatgatagat ggacgatggt gtgactgcaa acttcctaat tctaagcaaa
     1141 gccaagatga gcctttgaga agcagaaaag tgtttgtggg gcgctgtaca gaggacatga
     1201 ctgaggatga gctgcgggag ttcttctctc agtacgggga tgtgatggat gtcttcatcc
     1261 ccaagccatt cagggccttt gcctttgtta catttgcaga tgatcagatt gcgcagtctc
     1321 tttgtggaga ggacttgatc attaaaggaa tcagcgttca tatatccaat gccgaaccta
     1381 agcacaatag caatagacag ttagaaagaa gtggaagatt tggtggtaat ccaggtggct
     1441 ttgggaatca ggctggattt ggtaatagca gagggggtgg agctggtttg ggaaacaatc
     1501 aaggtagtaa tatgggtggt gggatgaact ttggtgcgtt cagcattaat ccagccatga
     1561 tggctgccgc ccaggcagca ctacagagca gttggggtat gatgggcatg ttagccagcc
     1621 agcagaacca gtcaggccca tcgggtaata accaaaacca aggcaacatg cagagggagc
     1681 caaaccaggc cttcggttct ggaaataact cttatagtgg ctctaattct ggtgcagcaa
     1741 ttggttgggg atcagcatcc aatgcagggt cgggcagtgg ttttaatgga ggctttggct
     1801 caagcatgga ttctaagtct tctggctggg gaatgttgga cccagctttc ttgtacaaag
     1861 tggttgattc tagagcgatc gccagagagc tgttcaacct gctgggcgag aaccagcccc
     1921 ccgtggtgat caaggcgatc gcctggagcc accctcagtt cgagaaggga ggaggaagcg
     1981 gcggaggcag cggaggagga agctggagcc acccgcagtt cgagaaaggc ggaagcggcc
     2041 atcaccatca ccatcaccct gcaggagaaa atctgtactt ccaggggaga ggaggcagcg
     2101 agaacctgta ctttcagggg cctgcaggaa gagactacaa agaccatgac ggtgattata
     2161 aagatcatga catcgattac aaggatgacg atgacaagta gttcgaattc tcgacctcga
     2221 gacaaatggc agtatcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2281 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2341 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc
     2401 gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac
     2461 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat
     2521 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg
     2581 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga
     2641 cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag
     2701 caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg
     2761 ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg
     2821 ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag
     2881 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta
     2941 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     3001 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     3061 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     3121 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     3181 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     3241 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     3301 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     3361 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     3421 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     3481 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     3541 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg
     3601 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3661 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     3721 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3781 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3841 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3901 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3961 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4021 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4081 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     4141 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4201 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4261 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     4321 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4381 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4441 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     4501 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4561 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4621 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4681 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4741 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4801 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4861 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4921 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4981 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5041 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5101 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5161 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5221 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5281 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5341 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5401 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5461 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5521 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5581 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5641 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5701 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5761 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5821 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5881 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5941 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6001 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6241 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6721 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6781 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6841 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6901 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6961 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7021 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7081 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     7141 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     7201 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     7261 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     7321 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     7381 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7441 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7501 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7561 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     7621 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     7681 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     7741 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     7801 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     7861 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     7921 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     7981 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     8041 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     8101 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     8161 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     8221 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     8281 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     8341 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     8401 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     8461 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     8521 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     8581 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     8641 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     8701 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     8761 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     8821 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     8881 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     8941 gtatcgatca cgagactagc ctcgagcggc cgcccccttc acc
//
