LOCUS       pEGFP-Myo10_FERM        6444 bp    DNA     circular UNA 17-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...2n-9jxvk8g
VERSION     urn.local...2n-9jxvk8g
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..589
                     /note="Geneious type: promoter uukaryotic"
     misc_feature    606..616
     CDS             613..1329
     primer_bind     1266..1287
     misc_feature    order(<3089..3130,1330..>1363)
     misc_feature    1360..1363
                     /note="Geneious type: ligation"
     misc_feature    1364..3088
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1365
                     /note="Geneious type: TAG"
     CDS             <1366..3084
     misc_feature    <1366..1410
     primer_bind     1366..1382
     gene            1999..2283
     misc_feature    2554..2559
     misc_feature    2581..2592
     primer_bind     complement(3070..3087)
     misc_feature    3089..3092
                     /note="Geneious type: ligation"
     misc_feature    3093..1359
                     /note="Geneious type: Concatenated sequence"
     misc_feature    complement(3113..3139)
                     /note="Geneious type: primer"
     gene            complement(3424..3443)
     CDS             4340..5131
ORIGIN      
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attcaacccg gtggtccagt
     1381 gccattcaaa acgtgactga caccaaggcc ccgatcgaca cccccaccca gcagctgatt
     1441 caagatatca aggagaactg cctgaactcg gatgtggtgg aacagattta caagcggaac
     1501 ccgatccttc gatacaccca tcaccccttg cactccccgc tcctgcccct tccgtatggg
     1561 gacataaatc tcaacttgct caaagacaaa ggctatacca cccttcagga tgaggccatc
     1621 aagatattca attccctgca gcaactggag tccatgtctg acccaattcc aataatccag
     1681 ggcatcctac agacagggca tgacctgcga cctctgcggg acgagctgta ctgccagctt
     1741 atcaaacaga ccaacaaagt gccccacccc ggcagtgtgg gcaacctgta cagctggcag
     1801 atcctgacat gcctgagctg caccttcctg ccgagtcgag ggattctcaa gtatctcaag
     1861 ttccatctga aaaggatacg ggaacagttt ccaggaaccg agatggaaaa atacgctctc
     1921 ttcacttacg aatctcttaa gaaaaccaaa tgccgagagt ttgtgccttc ccgagatgaa
     1981 atagaagctc tgatccacag gcaggaaatg acatccacgg tctattgcca tggcggcggc
     2041 tcttgcaaga tcaccatcaa ctcccacacc actgctgggg aggtggtgga gaagctgatc
     2101 cgaggcctgg ccatggagga cagcaggaac atgtttgctt tgtttgaata caacggccac
     2161 gtcgacaaag ccattgaaag tcgaaccgtc gtagctgatg tcttagccaa gtttgaaaag
     2221 ctggctgcca catccgaggt tggggacctg ccatggaaat tctacttcaa actttactgc
     2281 ttcctggaca cagacaacgt gccaaaagac agtgtggagt ttgcatttat gtttgaacag
     2341 gcccacgaag cggttatcca tggccaccat ccagccccgg aagaaaacct ccaggttctt
     2401 gctgccctgc gactccagta tctgcagggg gattatactc tgcacgctgc catcccacct
     2461 ctcgaagagg tttattccct gcagagactc aaggcccgca tcagccagtc aaccaaaacc
     2521 ttcacccctt gtgaacggct ggagaagagg cggacgagct tcctagaggg gaccctgagg
     2581 cggagcttcc ggacaggatc cgtggtccgg cagaaggtcg aggaggagca gatgctggac
     2641 atgtggatta aggaagaagt ctcctctgct cgagccagta tcattgacaa gtggaggaaa
     2701 tttcagggca tgagccagga acaggccatg gccaagtaca tggccttgat caaggagtgg
     2761 cctggctatg gctcgacgct gtttgatgtg gagtgcaagg aaggtggctt ccctcaggaa
     2821 ctctggttgg gtgtcagcgc ggacgacgtc tccgtctaca agcgtggaga gggaagacca
     2881 ctggaagtct tccagtatga acacatcctc tcttttgggg cacccctggc gaatacgtat
     2941 aagatcgtgg tcgatgagag ggagctgctc tttgaaacca gtgaggtggt ggatgtggcc
     3001 aagctcatga aagcctacat cagcatgatc gtgaagaagc gctacagcac gacacgctcc
     3061 gccagcagcc agggcagctc caggtgaggt accgcgggcc cgggatccac cggatctaga
     3121 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     3181 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     3241 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3301 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     3361 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     3421 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     3481 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     3541 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     3601 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     3661 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     3721 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     3781 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     3841 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3901 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     3961 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4021 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4081 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4141 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4201 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     4261 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     4321 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4381 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4441 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     4501 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     4561 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     4621 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     4681 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     4741 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     4801 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     4861 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     4921 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     4981 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5041 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5101 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     5161 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     5221 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     5281 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     5341 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     5401 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     5461 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     5521 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     5581 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5641 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5701 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5761 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5821 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5881 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5941 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6001 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6061 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6121 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6181 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6241 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6301 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6361 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6421 gataaccgta ttaccgccat gcat
//
