LOCUS       Exported                6161 bp ds-DNA     circular SYN 18-MAR-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pGAL4-DBD-HOXD13wt-IDR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 18, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    944..946
                     /label=start_codon
     CDS             947..1384
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     misc_feature    1394..1762
                     /label=hoxd13_wt_IDR
     promoter        complement(1787..1805)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1788..1805)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1825..1842)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer.
                     Also called BGH reverse"
     polyA_signal    1831..2055
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2101..2529
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2188..2207)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2398..2419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2538..2558)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2543..2873
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2724..2859
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2786..2805
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2940..3734
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2994..3013)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3604..3623
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3908..4029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3945..3964)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3999..4018
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4078..4094)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4078..4094)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4091..4113)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4102..4118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4126..4156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4171..4192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(4309..4326)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4480..5068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4560..4579)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5239..6099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5862..5881
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcatgaagc tactgtcttc
      961 tatcgaacaa gcatgcgata tttgccgact taaaaagctc aagtgctcca aagaaaaacc
     1021 gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc tactctccca aaaccaaaag
     1081 gtctccgctg actagggcac atctgacaga agtggaatca aggctagaaa gactggaaca
     1141 gctatttcta ctgatttttc ctcgagaaga ccttgacatg attttgaaaa tggattcttt
     1201 acaggatata aaagcattgt taacaggatt atttgtacaa gataatgtga ataaagatgc
     1261 cgtcacagat agattggctt cagtggagac tgatatgcct ctaacattga gacagcatag
     1321 aataagtgcg acatcatcat cggaagagag tagtaacaaa ggtcaaagac agttgactgt
     1381 atcggcgatc gcttcccgag ccggtagctg ggacatggac ggcctgcgag cggacggagg
     1441 cggagctgga ggagctcccg catcaagtag tagctcaagt gtggccgctg ccgcggctag
     1501 cggacaatgt agggggtttt tgtcagcgcc tgtctttgcg ggcacacatt ccgggagggc
     1561 cgctgctgcg gctgctgctg ctgctgcagc cgcggcggcc gctagtggat ttgcctatcc
     1621 tgggacgagt gagcgcactg gttcatcatc ctcatcttca tccagtgcgg tagtcgccgc
     1681 tcggccagaa gcaccacctg caaaagagtg tccggccccg acgccagctg cagcagcagc
     1741 ggcaccgcct tcagctcctg cgtgacgtac gtctagaggg ccctattcta tagtgtcacc
     1801 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     1861 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     1921 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     1981 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     2041 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     2101 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     2161 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     2221 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta
     2281 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     2341 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     2401 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     2461 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     2521 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     2581 aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     2641 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2701 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     2761 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     2821 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     2881 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     2941 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     3001 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     3061 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3121 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3181 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3241 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3301 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3361 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3421 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3481 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3541 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3601 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3661 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3721 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3781 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3841 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3901 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3961 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4021 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     4081 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4141 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4201 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4261 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4321 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4381 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4441 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4501 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4561 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4621 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     4681 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4741 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4801 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4861 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4921 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4981 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5041 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5101 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5161 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5221 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5281 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5341 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5401 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5461 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5521 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5581 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5641 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5701 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5761 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5821 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5881 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5941 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6001 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6061 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6121 ttgaagcatt tatcagggtt attgtctcat gagcggatac a
//
