LOCUS       Exported               12606 bp ds-DNA     circular SYN 08-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12606)
  AUTHORS   Dept. Meissner
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12606
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..686
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     645..667
                     /label=pHR_fw
     CDS             795..1147
                     /codon_start=1
                     /label=hoxa13 idr
                     /translation="MELNKNMEGAAAAAAAAAAAAAAGAGGGGFPHPAAAAAGGNFSVA
                     AAAAAAAAAAANQCRNLMAHPAPLAPGAASAYSSAPGEAPPSAAAAAAAAAAAAAAAAA
                     AAAAAAAASSSGG"
     CDS             1122..1412
                     /codon_start=1
                     /label=hoxa13 idr
                     /translation="AAASSSGGPGPAGPAGAEAAKQCSPCSAAAQSSSGPAALPYGYFG
                     SGYYPCARMGPHPNAIKSCAQPASAAAAAAFADKYMDTAGPAAEEFSSRAKE"
     CDS             1428..1492
                     /codon_start=1
                     /label=mcherry
                     /translation="MVSKGEEDNMAIIKEFMRFKV"
     primer_bind     complement(1747..1767)
                     /label=pHR_re4
     CDS             3702..3761
                     /codon_start=1
                     /label=product
                     /translation="GTPTCNMRDGGGPKKKRKV"
     CDS             3738..3758
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3823..4411
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3876..3896)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4294..4305)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4414..4430)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4415..4431)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4521..4754
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4779..4797)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4780..4797)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5087..5106)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5320..5339)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5439..5461
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5499..5517)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5585..5689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5690..6550
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5908..5927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6721..7309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7210..7229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7773..7795)
                     /label=pHR_fw
     primer_bind     complement(8190..8210)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8195..8524
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8375..8510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8437..8456
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8460..8471)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          9658..9723
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9853..9873
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10298..10432
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10335..10354)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10389..10408
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10600..11233
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11280..11405
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11902..12135
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      781 gatccggagc taccatggag ttgaacaaaa acatggaggg ggcggctgcg gctgcagccg
      841 cggcagcggc tgcagcggct gcaggagccg gtggaggagg ttttccccat ccggcggcgg
      901 ctgccgcggg ggggaatttc agtgttgcgg cagctgcagc agctgcagct gctgccgcag
      961 ctaaccagtg ccgcaacctg atggcgcatc cagcgcctct tgcgccgggg gccgcatcag
     1021 cgtacagttc tgctcctggg gaagcacccc cgtccgcggc tgctgccgcg gcagcggcag
     1081 cagcagccgc ggctgcggca gctgcagctg cggccgctgc cgcggcagct agctccagtg
     1141 gaggtcccgg accagcggga cctgctggtg cggaagccgc gaagcagtgt agcccgtgca
     1201 gcgcggcagc tcagagctca tccggtcccg ccgccctccc atacggatac tttggctcag
     1261 ggtactaccc gtgcgcgaga atgggccctc accccaacgc cataaagtca tgtgcccaac
     1321 ccgcgtcagc cgcagcggca gcagcttttg ccgataagta catggacact gctggccccg
     1381 cggcggagga gttcagtagc agggcgaagg agggtaccgg aggcgggatg gtttccaaag
     1441 gagaggagga taatatggct ataattaaag agtttatgcg gttcaaggtg catatggagg
     1501 gatctgtgaa cggtcacgag ttcgaaatcg agggcgaagg cgaggggcga ccctatgaag
     1561 ggacacagac ggccaaactg aaggtgacca agggtggccc cctgcctttc gcctgggaca
     1621 tcctgtcacc ccagtttatg tacggctcta aggcttacgt taagcaccct gccgatattc
     1681 cggactactt gaaactgagc ttccctgaag ggttcaagtg ggaacgggta atgaattttg
     1741 aggatggcgg tgtagtcaca gttacccagg acagctctct tcaggacgga gaatttatct
     1801 ataaggttaa acttcggggc actaacttcc catcagacgg ccccgtgatg cagaagaaaa
     1861 ctatgggctg ggaagccagc tcagaacgca tgtatcccga ggacggagca ctgaagggag
     1921 aaatcaagca gcgactcaag ctgaaggatg ggggacatta tgatgctgag gtcaaaacca
     1981 cctataaggc caagaaaccc gttcagcttc ctggggcata caacgtgaat atcaaactgg
     2041 atattacatc ccataacgaa gactatacca tcgtggaaca gtacgagcgg gccgagggca
     2101 gacatagcac agggggcatg gatgaattgt acaaggcggc cacgcgtatg aagatggaca
     2161 aaaagactat agtttggttt agaagagacc taaggattga ggataatcct gcattagcag
     2221 cagctgctca cgaaggatct gtttttcctg tcttcatttg gtgtcctgaa gaagaaggac
     2281 agttttatcc tggaagagct tcaagatggt ggatgaaaca atcacttgct cacttatctc
     2341 aatccttgaa ggctcttgga tctgacctca ctttaatcaa aacccacaac acgatttcag
     2401 cgatcttgga ttgtatccgc gttaccggtg ctacaaaagt cgtctttaac cacctctatg
     2461 atcctgtttc gttagttcgg gaccataccg taaaggagaa gctggtggaa cgtgggatct
     2521 ctgtgcaaag ctacaatgga gatctattgt atgaaccgtg ggagatatac tgcgaaaagg
     2581 gcaaaccttt tacgagtttc aattcttact ggaagaaatg cttagatatg tcgattgaat
     2641 ccgttatgct tcctcctcct tggcggttga tgccaataac tgcagcggct gaagcgattt
     2701 gggcgtgttc gattgaagaa ctagggctgg agaatgaggc cgagaaaccg agcaatgcgt
     2761 tgttaactag agcttggtct ccaggatgga gcaatgctga taagttacta aatgagttca
     2821 tcgagaagca gttgatagat tatgcaaaga acagcaagaa agttgttggg aattctactt
     2881 cactactttc tccgtatctc catttcgggg aaataagcgt cagacacgtt ttccagtgtg
     2941 cccggatgaa acaaattata tgggcaagag ataagaacag tgaaggagaa gaaagtgcag
     3001 atctttttct taggggaatc ggtttaagag agtattctcg gtatatatgt ttcaacttcc
     3061 cgtttactca cgagcaatcg ttgttgagtc atcttcggtt tttcccttgg gatgctgatg
     3121 ttgataagtt caaggcctgg agacaaggca ggaccggtta tccgttggtg gatgccggaa
     3181 tgagagagct ttgggctacc ggatggatgc ataacagaat aagagtgatt gtttcaagct
     3241 ttgctgtgaa gtttcttctc cttccatgga aatggggaat gaagtatttc tgggatacac
     3301 ttttggatgc tgatttggaa tgtgacatcc ttggctggca gtatatctct gggagtatcc
     3361 ccgatggcca cgagcttgat cgcttggaca atcccgcgtt acaaggcgcc aaatatgacc
     3421 cagaaggtga gtacataagg caatggcttc ccgagcttgc gagattgcca actgaatgga
     3481 tccatcatcc atgggacgct cctttaaccg tactcaaagc ttctggtgtg gaactcggaa
     3541 caaactatgc gaaacccatt gtagacatcg acacagctcg tgagctacta gctaaagcta
     3601 tttcaagaac ccgtgaagca cagatcatga tcggagcagc agcccgggat ccaccggtcg
     3661 ccaccggttc agggagcgga tccggctcag ggagtgcggc cggaactccc acctgcaaca
     3721 tgcgtgacgg aggcggtcca aaaaagaaga gaaaggtatg actgaggccg cgactctaga
     3781 gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac
     3841 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3901 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3961 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4021 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4081 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4141 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4201 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4261 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4321 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4381 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa
     4441 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4501 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     4561 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4621 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4681 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4741 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     4801 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     4861 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     4921 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     4981 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     5041 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     5101 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     5161 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     5221 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     5281 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
     5341 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5401 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5461 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5521 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5581 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5641 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5701 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5761 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5821 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5881 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5941 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6001 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6061 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6121 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6181 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6241 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6301 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6361 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6421 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6481 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6541 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6601 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6661 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6721 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6781 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6841 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6901 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6961 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7021 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7081 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7141 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7201 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7261 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7321 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7381 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7441 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7501 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg gccatttaat
     7561 gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt gaaagacccc
     7621 cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga gaccacaagt
     7681 cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact cagtcaatcg
     7741 gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg ggagcagaag
     7801 cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca cagggtcatt
     7861 tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc tgagggctgg
     7921 accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg cttaccacag
     7981 atatcctgtt tggcccatat tcagccatgt tctttcctgc gttatcccct gattctgtgg
     8041 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8101 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8161 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8221 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8281 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8341 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8401 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8461 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8521 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     8581 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     8641 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     8701 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     8761 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     8821 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     8881 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     8941 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     9001 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     9061 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     9121 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     9181 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     9241 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     9301 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     9361 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     9421 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     9481 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     9541 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     9601 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     9661 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     9721 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     9781 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     9841 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     9901 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     9961 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
    10021 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
    10081 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
    10141 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
    10201 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
    10261 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    10321 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10381 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    10441 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    10501 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    10561 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
    10621 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
    10681 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
    10741 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
    10801 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
    10861 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
    10921 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
    10981 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
    11041 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    11101 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    11161 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    11221 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    11281 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    11341 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    11401 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    11461 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    11521 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    11581 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    11641 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    11701 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
    11761 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
    11821 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
    11881 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
    11941 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
    12001 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
    12061 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
    12121 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
    12181 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
    12241 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
    12301 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
    12361 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
    12421 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
    12481 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
    12541 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
    12601 agtgaa
//
