LOCUS       Exported               11828 bp ds-DNA     circular SYN 25-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11828)
  AUTHORS   Vahid Asimi
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11828
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..686
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     774..793
                     /label=Primer 1
     misc_feature    795..1274
                     /label=IDR
     misc_feature    966..1079
                     /label=PolyQ
     gene            1355..1997
                     /label=mCherry (full)
     gene            2010..3503
                     /label=Cry2-PHR (1-498)
     CDS             3564..3623
                     /codon_start=1
                     /label=product
                     /translation="GTPTCNMRDGGGPKKKRKV"
     CDS             3600..3620
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3685..4273
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3738..3758)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4156..4167)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4276..4292)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4277..4293)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4383..4616
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4641..4659)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4642..4659)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4949..4968)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5182..5201)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5301..5323
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5361..5379)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5447..5551
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5552..6412
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5770..5789)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6583..7171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7072..7091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7325..7342
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7412..7432)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7417..7746
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7597..7732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7659..7678
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7682..7693)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          8880..8945
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9075..9095
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9520..9654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9557..9576)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9611..9630
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9822..10455
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10502..10627
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11124..11357
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      781 gatccggagc taccatggat cagaacaaca gcctgccacc ttacgctcag ggcttggcct
      841 cccctcaggg tgccatgact cccggaatcc ctatctttag tccaatgatg ccttatggca
      901 ctggactgac cccacagcct attcagaaca ccaatagtct gtctattttg gaagagcaac
      961 aaaggcagca gcagcaacaa caacagcagc agcagcagca gcagcagcaa cagcaacagc
     1021 agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcaacagg
     1081 cagtggcagc tgcagccgtt cagcagtcaa cgtcccagca ggcaacacag ggaacctcag
     1141 gccaggcacc acagctcttc cactcacaga ctctcacaac tgcacccttg ccgggcacca
     1201 ctccactgta tccctccccc atgactccca tgacccccat cactcctgcc acgccagctt
     1261 cggagagttc tgggggtacc ggaggcggga tggtttccaa aggagaggag gataatatgg
     1321 ctataattaa agagtttatg cggttcaagg tgcatatgga gggatctgtg aacggtcacg
     1381 agttcgaaat cgagggcgaa ggcgaggggc gaccctatga agggacacag acggccaaac
     1441 tgaaggtgac caagggtggc cccctgcctt tcgcctggga catcctgtca ccccagttta
     1501 tgtacggctc taaggcttac gttaagcacc ctgccgatat tccggactac ttgaaactga
     1561 gcttccctga agggttcaag tgggaacggg taatgaattt tgaggatggc ggtgtagtca
     1621 cagttaccca ggacagctct cttcaggacg gagaatttat ctataaggtt aaacttcggg
     1681 gcactaactt cccatcagac ggccccgtga tgcagaagaa aactatgggc tgggaagcca
     1741 gctcagaacg catgtatccc gaggacggag cactgaaggg agaaatcaag cagcgactca
     1801 agctgaagga tgggggacat tatgatgctg aggtcaaaac cacctataag gccaagaaac
     1861 ccgttcagct tcctggggca tacaacgtga atatcaaact ggatattaca tcccataacg
     1921 aagactatac catcgtggaa cagtacgagc gggccgaggg cagacatagc acagggggca
     1981 tggatgaatt gtacaaggcg gccacgcgta tgaagatgga caaaaagact atagtttggt
     2041 ttagaagaga cctaaggatt gaggataatc ctgcattagc agcagctgct cacgaaggat
     2101 ctgtttttcc tgtcttcatt tggtgtcctg aagaagaagg acagttttat cctggaagag
     2161 cttcaagatg gtggatgaaa caatcacttg ctcacttatc tcaatccttg aaggctcttg
     2221 gatctgacct cactttaatc aaaacccaca acacgatttc agcgatcttg gattgtatcc
     2281 gcgttaccgg tgctacaaaa gtcgtcttta accacctcta tgatcctgtt tcgttagttc
     2341 gggaccatac cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa agctacaatg
     2401 gagatctatt gtatgaaccg tgggagatat actgcgaaaa gggcaaacct tttacgagtt
     2461 tcaattctta ctggaagaaa tgcttagata tgtcgattga atccgttatg cttcctcctc
     2521 cttggcggtt gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag
     2581 aactagggct ggagaatgag gccgagaaac cgagcaatgc gttgttaact agagcttggt
     2641 ctccaggatg gagcaatgct gataagttac taaatgagtt catcgagaag cagttgatag
     2701 attatgcaaa gaacagcaag aaagttgttg ggaattctac ttcactactt tctccgtatc
     2761 tccatttcgg ggaaataagc gtcagacacg ttttccagtg tgcccggatg aaacaaatta
     2821 tatgggcaag agataagaac agtgaaggag aagaaagtgc agatcttttt cttaggggaa
     2881 tcggtttaag agagtattct cggtatatat gtttcaactt cccgtttact cacgagcaat
     2941 cgttgttgag tcatcttcgg tttttccctt gggatgctga tgttgataag ttcaaggcct
     3001 ggagacaagg caggaccggt tatccgttgg tggatgccgg aatgagagag ctttgggcta
     3061 ccggatggat gcataacaga ataagagtga ttgtttcaag ctttgctgtg aagtttcttc
     3121 tccttccatg gaaatgggga atgaagtatt tctgggatac acttttggat gctgatttgg
     3181 aatgtgacat ccttggctgg cagtatatct ctgggagtat ccccgatggc cacgagcttg
     3241 atcgcttgga caatcccgcg ttacaaggcg ccaaatatga cccagaaggt gagtacataa
     3301 ggcaatggct tcccgagctt gcgagattgc caactgaatg gatccatcat ccatgggacg
     3361 ctcctttaac cgtactcaaa gcttctggtg tggaactcgg aacaaactat gcgaaaccca
     3421 ttgtagacat cgacacagct cgtgagctac tagctaaagc tatttcaaga acccgtgaag
     3481 cacagatcat gatcggagca gcagcccggg atccaccggt cgccaccggt tcagggagcg
     3541 gatccggctc agggagtgcg gccggaactc ccacctgcaa catgcgtgac ggaggcggtc
     3601 caaaaaagaa gagaaaggta tgactgaggc cgcgactcta gagtcgacct gcaggcatgc
     3661 aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     3721 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3781 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3841 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3901 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3961 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4021 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4081 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4141 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4201 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4261 gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt
     4321 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4381 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     4441 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4501 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4561 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     4621 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     4681 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     4741 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     4801 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     4861 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     4921 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     4981 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     5041 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     5101 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     5161 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     5221 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     5281 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     5341 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5401 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5461 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5521 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5581 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5641 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5701 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5761 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5821 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5881 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5941 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6001 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6061 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6121 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6181 ggcggataaa gttgcaggaa cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6241 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6301 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6361 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6421 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6481 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6541 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6601 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6661 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6721 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6781 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6841 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6901 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6961 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7021 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7081 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7141 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7201 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7261 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7321 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7381 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     7441 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7501 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7561 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7621 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7681 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7741 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     7801 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     7861 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     7921 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     7981 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     8041 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
     8101 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     8161 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     8221 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     8281 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     8341 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     8401 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
     8461 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     8521 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     8581 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
     8641 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
     8701 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
     8761 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
     8821 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     8881 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     8941 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     9001 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     9061 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     9121 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     9181 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     9241 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     9301 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     9361 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     9421 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     9481 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     9541 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     9601 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
     9661 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
     9721 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     9781 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     9841 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     9901 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     9961 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
    10021 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
    10081 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
    10141 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
    10201 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
    10261 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    10321 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    10381 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    10441 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
    10501 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
    10561 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
    10621 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
    10681 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
    10741 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
    10801 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
    10861 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
    10921 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc
    10981 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
    11041 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
    11101 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
    11161 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
    11221 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
    11281 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
    11341 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
    11401 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
    11461 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
    11521 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
    11581 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
    11641 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
    11701 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
    11761 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
    11821 ttagtgaa
//
