LOCUS       Exported               11616 bp ds-DNA     circular SYN 24-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..686
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     670..752
                     /label=Primer 1
     misc_feature    730..1359
                     /label=RY6878-insert
     misc_feature    1105..2607
                     /label=Translated_long_Med1
     primer_bind     complement(2583..2626)
                     /label=Primer 2
     primer_bind     2625..2647
                     /label=NdeI_fw
     CDS             2629..3342
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3323..3394)
                     /label=NotI_re
     CDS             3352..3372
                     /codon_start=1
                     /label=SV40x1 NLS
                     /translation="PKKKRKV"
     misc_feature    3473..4061
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3526..3546)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3944..3955)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4064..4080)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4065..4081)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4171..4404
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4429..4447)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4430..4447)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4737..4756)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4970..4989)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5089..5111
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5149..5167)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5235..5339
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5340..6200
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5558..5577)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6371..6959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6860..6879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7113..7130
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7200..7220)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7205..7534
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7385..7520
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7447..7466
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7470..7481)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          8668..8733
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8863..8883
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9308..9442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9345..9364)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9399..9418
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9610..10243
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10290..10415
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10912..11145
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agcatcacag tggtagtcag ggtcctttac tgaccactgg ggacttaggg
      781 aaagaaaaga ctcaaaagag ggtaaaggaa ggcaatggca ccagtaatag tactctctcg
      841 gggcccggat tagacagcaa accagggaag cgcagtcgga ccccttctaa tgatgggaaa
      901 agcaaagata agcctccaaa gcggaagaag gcagacactg agggaaagtc tccatctcat
      961 agttcttcta acagaccttt taccccacct accagtacag gtggatctaa atcgccaggc
     1021 agtgcaggaa gatctcagac tcccccaggt gttgccacac cacccattcc caaaatcact
     1081 attcagattc ctaagggaac agtgatggtg ggcaagcctt cctctcacag tcagtatacc
     1141 agcagtggtt ctgtgtcttc ctcaggcagc aaaagccacc atagccattc ttcctcctct
     1201 tcctcatctg cttccacctc agggaagatg aaaagcagta aatcagaagg ttcatcaagt
     1261 tccaagttaa gtagcagtat gtattctagc caggggtctt ctggatctag ccagtccaaa
     1321 aattcatccc agtctggggg gaagccaggc tcctctccca taaccaagca tggactgagc
     1381 agtggctcta gcagcaccaa gatgaaacct caaggaaagc catcatcact tatgaatcct
     1441 tctttaagta aaccaaacat atccccttct cattcaaggc cacctggagg ctctgacaag
     1501 cttgcctctc caatgaagcc tgttcctgga actcctccat cctctaaagc caagtcccct
     1561 atcagttcag gttctggtgg ttctcatatg tctggaacta gttcaagctc tggcatgaag
     1621 tcatcttcag ggttaggatc ctcaggctcg ttgtcccaga aaactccccc atcatctaat
     1681 tcctgtacgg catcttcctc ctccttttcc tcaagtggct cttccatgtc atcctctcag
     1741 aaccagcatg ggagttctaa aggaaaatct cccagcagaa acaagaagcc gtccttgaca
     1801 gctgtcatag ataaactgaa gcatggggtt gtcaccagtg gccctggggg tgaagaccca
     1861 ctggacggcc agatgggggt gagcacaaat tcttccagcc atcctatgtc ctccaaacat
     1921 aacatgtcag gaggagagtt tcagggcaag cgtgagaaaa gtgataaaga caaatcaaag
     1981 gtttccacct ccgggagttc agtggattct tctaagaaga cctcagagtc aaaaaatgtg
     2041 gggagcacag gtgtggcaaa aattatcatc agtaagcatg atggaggctc ccctagcatt
     2101 aaagccaaag tgactttgca gaaacctggg gaaagtagtg gagaagggct taggcctcaa
     2161 atggcttctt ctaaaaacta tggctctcca ctcatcagtg gttccactcc aaagcatgag
     2221 cgtggctctc ccagccatag taagtcacca gcatataccc cccagaatct ggacagtgaa
     2281 agtgagtcag gctcctccat agcagagaaa tcttatcaga atagtcccag ctcagacgat
     2341 ggtatccgac cacttccaga atacagcaca gagaaacata agaagcacaa aaaggaaaag
     2401 aagaaagtaa aagacaaaga tagggaccga gaccgggaca aagaccgaga caagaaaaaa
     2461 tctcatagca tcaagccaga gagttggtcc aaatcaccca tctcttcaga ccagtccttg
     2521 tctatgacaa gtaacacaat cttatctgca gacagaccct caaggctcag cccagacttt
     2581 atgattgggg aggaagatga tgatcttgga ggtagcggag gaattatggt gagcaagggc
     2641 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2701 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2761 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     2821 ggctacggcc tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     2881 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     2941 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3001 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3061 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3121 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     3181 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     3241 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3301 accgccgccg ggatcactct cggcatggac gagctgtaca agggaggcgg tccaaaaaag
     3361 aagagaaagg tatgagcggc cgcaactccc acctgcaaca tgcgtgactg actgaggccg
     3421 cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
     3481 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3541 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3601 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3661 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     3721 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     3781 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3841 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     3901 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3961 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4021 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     4081 agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     4141 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4201 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4261 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4321 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4381 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
     4441 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
     4501 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
     4561 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
     4621 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
     4681 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     4741 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     4801 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     4861 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     4921 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     4981 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5041 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5101 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     5161 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     5221 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5281 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5341 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5401 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5461 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5521 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5581 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5641 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5701 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5761 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5821 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5881 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5941 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggaaca cttctgcgct
     6001 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6061 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6121 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6181 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6241 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6301 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6361 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6421 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6481 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6541 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6601 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6661 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6721 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6781 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6841 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6901 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6961 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7021 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7081 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7141 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     7201 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7261 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7321 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7381 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7441 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7501 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     7561 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     7621 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     7681 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     7741 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     7801 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     7861 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     7921 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     7981 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     8041 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     8101 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     8161 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     8221 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     8281 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     8341 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     8401 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     8461 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     8521 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     8581 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     8641 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     8701 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     8761 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     8821 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     8881 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     8941 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     9001 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     9061 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     9121 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
     9181 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     9241 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     9301 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     9361 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9421 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     9481 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     9541 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     9601 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     9661 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     9721 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     9781 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     9841 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     9901 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     9961 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
    10021 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
    10081 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    10141 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    10201 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    10261 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    10321 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    10381 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    10441 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    10501 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    10561 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    10621 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    10681 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    10741 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    10801 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    10861 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    10921 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    10981 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    11041 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    11101 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    11161 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    11221 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    11281 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    11341 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    11401 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    11461 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    11521 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    11581 agagagagac agagacagat ccattcgatt agtgaa
//
