LOCUS       Exported               11693 bp ds-DNA     circular SYN 24-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Vahid Asimi
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11693
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..686
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     763..805
                     /label=Primer 1
     misc_feature    783..2660
                     /label=MED1 IDR
     misc_feature    783..1412
                     /label=fragment 1
     misc_feature    783..804
                     /label=LEFT PRIMERS
     primer_bind     complement(2636..2683)
                     /label=Primer 2
     misc_feature    2636..2660
                     /label=RIGHT PRIMERS
     primer_bind     2700..2724
                     /label=NdeI_fw
     CDS             2706..3419
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3400..3471)
                     /label=NotI_re
     CDS             3429..3449
                     /codon_start=1
                     /label=SV40x1 NLS
                     /translation="PKKKRKV"
     misc_feature    3550..4138
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3603..3623)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4021..4032)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4141..4157)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4142..4158)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4248..4481
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4506..4524)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4507..4524)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4814..4833)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5047..5066)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5166..5188
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5226..5244)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5312..5416
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5417..6277
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5635..5654)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6448..7036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6937..6956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7190..7207
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7277..7297)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7282..7611
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7462..7597
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7524..7543
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7547..7558)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          8745..8810
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8940..8960
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9385..9519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9422..9441)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9476..9495
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9687..10320
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10367..10492
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10989..11222
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 aagagaggcc actagtcgcg accacgcgcc ggcgggacga gacgcgatcg cgagctacca
      781 tggagcatca cagtggtagt cagggtcctt tactgaccac tggggactta gggaaagaaa
      841 agactcaaaa gagggtaaag gaaggcaatg gcaccagtaa tagtactctc tcggggcccg
      901 gattagacag caaaccaggg aagcgcagtc ggaccccttc taatgatggg aaaagcaaag
      961 ataagcctcc aaagcggaag aaggcagaca ctgagggaaa gtctccatct catagttctt
     1021 ctaacagacc ttttacccca cctaccagta caggtggatc taaatcgcca ggcagtgcag
     1081 gaagatctca gactccccca ggtgttgcca caccacccat tcccaaaatc actattcaga
     1141 ttcctaaggg aacagtgatg gtgggcaagc cttcctctca cagtcagtat accagcagtg
     1201 gttctgtgtc ttcctcaggc agcaaaagcc accatagcca ttcttcctcc tcttcctcat
     1261 ctgcttccac ctcagggaag atgaaaagca gtaaatcaga aggttcatca agttccaagt
     1321 taagtagcag tatgtattct agccaggggt cttctggatc tagccagtcc aaaaattcat
     1381 cccagtctgg ggggaagcca ggctcctctc ccataaccaa gcatggactg agcagtggct
     1441 ctagcagcac caagatgaaa cctcaaggaa agccatcatc acttatgaat ccttctttaa
     1501 gtaaaccaaa catatcccct tctcattcaa ggccacctgg aggctctgac aagcttgcct
     1561 ctccaatgaa gcctgttcct ggaactcctc catcctctaa agccaagtcc cctatcagtt
     1621 caggttctgg tggttctcat atgtctggaa ctagttcaag ctctggcatg aagtcatctt
     1681 cagggttagg atcctcaggc tcgttgtccc agaaaactcc cccatcatct aattcctgta
     1741 cggcatcttc ctcctccttt tcctcaagtg gctcttccat gtcatcctct cagaaccagc
     1801 atgggagttc taaaggaaaa tctcccagca gaaacaagaa gccgtccttg acagctgtca
     1861 tagataaact gaagcatggg gttgtcacca gtggccctgg gggtgaagac ccactggacg
     1921 gccagatggg ggtgagcaca aattcttcca gccatcctat gtcctccaaa cataacatgt
     1981 caggaggaga gtttcagggc aagcgtgaga aaagtgataa agacaaatca aaggtttcca
     2041 cctccgggag ttcagtggat tcttctaaga agacctcaga gtcaaaaaat gtggggagca
     2101 caggtgtggc aaaaattatc atcagtaagc atgatggagg ctcccctagc attaaagcca
     2161 aagtgacttt gcagaaacct ggggaaagta gtggagaagg gcttaggcct caaatggctt
     2221 cttctaaaaa ctatggctct ccactcatca gtggttccac tccaaagcat gagcgtggct
     2281 ctcccagcca tagtaagtca ccagcatata ccccccagaa tctggacagt gaaagtgagt
     2341 caggctcctc catagcagag aaatcttatc agaatagtcc cagctcagac gatggtatcc
     2401 gaccacttcc agaatacagc acagagaaac ataagaagca caaaaaggaa aagaagaaag
     2461 taaaagacaa agatagggac cgagaccggg acaaagaccg agacaagaaa aaatctcata
     2521 gcatcaagcc agagagttgg tccaaatcac ccatctcttc agaccagtcc ttgtctatga
     2581 caagtaacac aatcttatct gcagacagac cctcaaggct cagcccagac tttatgattg
     2641 gggaggaaga tgatgatctt ggaggtagcg gagcgtacgg gttctggctc gtcggttccc
     2701 atatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2761 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2821 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2881 ccctcgtgac caccttcggc tacggcctga tgtgcttcgc ccgctacccc gaccacatga
     2941 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3001 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3061 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3121 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     3181 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     3241 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3301 actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     3361 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     3421 gaggcggtcc aaaaaagaag agaaaggtat gagcggccgc aactcccacc tgcaacatgc
     3481 gtgactgact gaggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
     3541 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3601 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3661 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3721 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3781 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3841 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3901 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3961 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4021 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4081 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     4141 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
     4201 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4261 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     4321 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4381 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4441 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     4501 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     4561 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     4621 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     4681 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     4741 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     4801 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     4861 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     4921 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     4981 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     5041 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     5101 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5161 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     5221 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5281 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5341 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5401 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5461 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5521 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5581 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5641 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5701 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5761 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5821 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5881 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5941 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6001 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6061 aggaacactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6121 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6181 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6241 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6301 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6361 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6781 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6841 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7081 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7141 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7201 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7261 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     7321 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     7381 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     7441 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7561 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
     7621 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
     7681 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     7741 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     7801 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     7861 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     7921 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     7981 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     8041 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     8101 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     8161 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     8221 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     8281 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     8341 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     8401 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     8461 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     8521 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
     8581 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
     8641 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
     8701 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     8761 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     8821 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     8881 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     8941 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     9001 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     9061 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     9121 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     9181 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     9241 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     9301 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     9361 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     9421 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9481 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     9541 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     9601 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     9661 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     9721 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     9781 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     9841 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     9901 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     9961 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
    10021 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
    10081 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
    10141 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    10201 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    10261 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    10321 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
    10381 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
    10441 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
    10501 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
    10561 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
    10621 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
    10681 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
    10741 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
    10801 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
    10861 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    10921 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    10981 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    11041 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    11101 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    11161 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    11221 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    11281 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    11341 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    11401 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    11461 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    11521 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    11581 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    11641 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaa
//
