LOCUS       Exported                1087 bp ds-DNA     linear   SYN 31-MAR-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  PCR product
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Dept. Meissner
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="PCR product"
                     /mol_type="other DNA"
     source          8..1078
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     1..27
                     /label=Primer 1
     promoter        8..9
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat
                     (LTR) promoter"
     regulatory      26..35
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     CDS             32..343
                     /codon_start=1
                     /label=RUNX2wt IDR
                     /translation="MASNSLFSTVTPCQQNFFWDPSTSRRFSPPSSSLQPGKMSDVSPV
                     VAAQQQQQQQQQQQQQQQQQQQQQQQEAAAAAAAAAAAAAAAAAVPRLRPPHDNRTMVE
                     "
     gene            371..1078
                     /label=mCherry (full)
     primer_bind     complement(1059..1087)
                     /label=Primer 2
ORIGIN
        1 taccggtgga agagaggcca ctagtgccac catggcatca aacagcctct tcagcacagt
       61 gacaccatgt cagcaaaact tcttttggga tccgagcacc agccggcgct tcagcccccc
      121 ctccagcagc ctgcagcccg gcaaaatgag cgacgtgagc ccggtggtgg ctgcgcaaca
      181 gcagcagcaa cagcagcagc agcaacagca gcagcagcag cagcaacagc agcagcagca
      241 gcaggaggcg gcggcggccg ctgcggcggc ggcggcggct gcggcggcgg cagctgcagt
      301 gccccggtta cggccgcccc acgacaaccg caccatggtg gagacgtacg gggtctggct
      361 cgtcgggtcc atggtttcca aaggagagga ggataatatg gctataatta aagagtttat
      421 gcggttcaag gtgcatatgg agggatctgt gaacggtcac gagttcgaaa tcgagggcga
      481 aggcgagggg cgaccctatg aagggacaca gacggccaaa ctgaaggtga ccaagggtgg
      541 ccccctgcct ttcgcctggg acatcctgtc accccagttt atgtacggct ctaaggctta
      601 cgttaagcac cctgccgata ttccggacta cttgaaactg agcttccctg aagggttcaa
      661 gtgggaacgg gtaatgaatt ttgaggatgg cggtgtagtc acagttaccc aggacagctc
      721 tcttcaggac ggagaattta tctataaggt taaacttcgg ggcactaact tcccatcaga
      781 cggccccgtg atgcagaaga aaactatggg ctgggaagcc agctcagaac gcatgtatcc
      841 cgaggacgga gcactgaagg gagaaatcaa gcagcgactc aagctgaagg atgggggaca
      901 ttatgatgct gaggtcaaaa ccacctataa ggccaagaaa cccgttcagc ttcctggggc
      961 atacaacgtg aatatcaaac tggatattac atcccataac gaagactata ccatcgtgga
     1021 acagtacgag cgggccgagg gcagacatag cacagggggc atggatgaat tgtacaagaa
     1081 gcttgcg
//
