LOCUS       Exported                1117 bp ds-DNA     linear   SYN 31-MAR-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  PCR product
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Dept. Meissner
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="PCR product"
                     /mol_type="other DNA"
     source          8..1108
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     1..27
                     /label=Primer 1
     promoter        8..9
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat
                     (LTR) promoter"
     regulatory      26..35
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     CDS             32..373
                     /codon_start=1
                     /label=RUNX2+10A-IDR
                     /translation="MASNSLFSTVTPCQQNFFWDPSTSRRFSPPSSSLQPGKMSDVSPV
                     VAAQQQQQQQQQQQQQQQQQQQQQQQEAAAAAAAAAAAAAAAAAAAAAAAAAAAVPRLR
                     PPHDNRTMVE"
     gene            401..1108
                     /label=mCherry (full)
     primer_bind     complement(1089..1117)
                     /label=Primer 2
ORIGIN
        1 taccggtgga agagaggcca ctagtgccac catggcatca aacagcctct tcagcacagt
       61 gacaccatgt cagcaaaact tcttttggga tccgagcacc agccggcgct tcagcccccc
      121 ctccagcagc ctgcagcccg gcaaaatgag cgacgtgagc ccggtggtgg ctgcgcaaca
      181 gcagcagcaa cagcagcagc agcaacagca gcagcagcag cagcaacagc agcagcagca
      241 gcaggaggcg gcggcagcgg ccgctgccgc agcggctgca gcggcggcgg ctgcggcggc
      301 ggcggcggct gcggcggcgg cagctgcagt gccccggtta cggccgcccc acgacaaccg
      361 caccatggtg gagacgtacg gggtctggct cgtcgggtcc atggtttcca aaggagagga
      421 ggataatatg gctataatta aagagtttat gcggttcaag gtgcatatgg agggatctgt
      481 gaacggtcac gagttcgaaa tcgagggcga aggcgagggg cgaccctatg aagggacaca
      541 gacggccaaa ctgaaggtga ccaagggtgg ccccctgcct ttcgcctggg acatcctgtc
      601 accccagttt atgtacggct ctaaggctta cgttaagcac cctgccgata ttccggacta
      661 cttgaaactg agcttccctg aagggttcaa gtgggaacgg gtaatgaatt ttgaggatgg
      721 cggtgtagtc acagttaccc aggacagctc tcttcaggac ggagaattta tctataaggt
      781 taaacttcgg ggcactaact tcccatcaga cggccccgtg atgcagaaga aaactatggg
      841 ctgggaagcc agctcagaac gcatgtatcc cgaggacgga gcactgaagg gagaaatcaa
      901 gcagcgactc aagctgaagg atgggggaca ttatgatgct gaggtcaaaa ccacctataa
      961 ggccaagaaa cccgttcagc ttcctggggc atacaacgtg aatatcaaac tggatattac
     1021 atcccataac gaagactata ccatcgtgga acagtacgag cgggccgagg gcagacatag
     1081 cacagggggc atggatgaat tgtacaagaa gcttgcg
//
