LOCUS       Exported               10352 bp ds-DNA     circular SYN 25-APR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    MT82 pRRL SFFV-Flag-GFP-IRES-neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Caroline Goujon
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 28, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10352
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label=bla AmpR promoter
                     /label=bla promoter
                     /note="AmpR promoter"
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /label=bla CDS
                     /note="AmpR
                     confers resistance to ampicillin, carbenicillin, and 
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    330..992
                     /label=AmpR
                     /note="AmpR"
     misc_feature    1087..1769
                     /label=colE1 origin
                     /note="colE1 origin"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori rep origin
                     /note="ori
                     high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP bindin
                     /label=CAP binding site protein bind
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site
                     CAP binding activates transcription in the presence of 
                     cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="lac promoter
                     promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator prot
                     /label=lac operator protein bind
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator
                     The lac repressor binds to the lac operator to inhibit 
                     transcription in E. coli. This inhibition can be relieved 
                     by adding lactose or isopropyl-beta-D-thiogalactopyranoside
                     (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
     promoter        2174..2192
                     /label=T3 promoter
                     /note="T3 promoter
                     promoter for bacteriophage T3 RNA polymerase"
     misc_feature    2306..2311
                     /label=SphI, BbuI, PaeI
                     /note="Geneious type: restriction site
                     Recognition_pattern=GCATG^C
                     annotation_group=GCATGC"
     misc_feature    complement(2331..2341)
                     /label=BaeI
                     /note="Geneious type: restriction site
                     Recognition_pattern=(10/15)ACNNNNGTAYC(12/7)
                     annotation_group=ACNNNNGTAYC"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="5' LTR (truncated)
                     truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2675..2812
                     /label=PSI
                     /note="PSI"
     misc_feature    3289..3523
                     /label=RRE
                     /note="RRE"
     misc_feature    3901..4037
                     /label=cPPT-CTS
                     /note="cPPT-CTS"
     promoter        4182..4589
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     4507..4524
                     /label=M90 fw
     primer_bind     4507..4524
                     /label=M90
     primer_bind     4592..4621
                     /label=oBB788
     gene            4600..7017
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /label=ACE2
                     /note="angiotensin I converting enzyme 2"
                     /db_xref="GeneID:59272"
                     /db_xref="HGNC:HGNC:13557"
                     /db_xref="MIM:300335"
     exon            4600..4785
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     sig_peptide     4600..4650
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="COORDINATES:same species:SignalP"
     mat_peptide     4651..6723
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /product="Processed angiotensin-converting enzyme 2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9BYF1.2)"
                     /experiment="experimental evidence, no additional details
                     recorded"
     exon            4786..4944
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            4945..5038
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            5039..5182
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     primer_bind     5100..5121
                     /label=oBB792
     exon            5183..5295
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            5296..5401
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            5402..5499
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            5500..5669
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            5670..5896
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     primer_bind     5697..5716
                     /label=oBB793
     exon            5897..6041
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            6042..6140
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            6141..6263
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            6264..6436
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     primer_bind     6270..6292
                     /label=oBB794
     exon            6437..6495
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            6496..6596
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            6597..6713
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            6714..6908
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     misc_feature    6820..6882
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9BYF1.2); 
                     transmembrane region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     exon            6909..7017
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(6990..7023)
                     /label=oBB789
     primer_bind     7018..7040
                     /label=oCG552 KCL
     misc_feature    7044..7605
                     /label=EMCV IRES
                     /note="EMCV IRES"
     misc_feature    7051..7603
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7123..7141)
                     /label=M23
     primer_bind     complement(7123..7141)
                     /label=M23a
     primer_bind     7513..7535
                     /label=M68
     primer_bind     complement(7585..7618)
                     /label=oCG553 KCL
     CDS             7615..8409
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    8412..8417
                     /label=SalI
                     /note="Geneious type: restriction site
                     Recognition_pattern=G^TCGAC
                     annotation_group=GTCGAC"
     misc_feature    8418..9006
                     /label=WPRE
                     /note="WPRE
                     woodchuck hepatitis virus posttranscriptional regulatory 
                     element"
     LTR             9093..9326
                     /label=3' LTR (Delta-U3)
                     /note="3' LTR (Delta-U3)
                     self-inactivating 3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9398..9519
                     /label=SV40 poly(A) signal polyA signal
                     /note="SV40 poly(A) signal
                     SV40 polyadenylation signal"
     rep_origin      9538..9673
                     /label=SV40 ori rep origin
                     /note="SV40 ori
                     SV40 origin of replication"
     promoter        complement(9706..9724)
                     /label=T7 promoter
                     /note="T7 promoter
                     promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9734..9750)
                     /label=M13 fwd primer bind
                     /note="M13 fwd
                     common sequencing primer, one of multiple similar variants"
     rep_origin      9895..10350
                     /direction=RIGHT
                     /label=f1 ori rep origin
                     /note="f1 ori
                     f1 bacteriophage origin of replication; arrow indicates 
                     direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggccccgaac
     2641 agggacctga aagcgaaagg gaaaccagag ctctctcgac gcaggactcg gcttgctgaa
     2701 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     2761 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     2821 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     2881 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2941 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     3001 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3061 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3121 accgcacagc aaggggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     3181 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     3241 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     3301 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcctcaatga cgctgacggt
     3361 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     3421 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     3481 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     3541 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     3601 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     4141 taatgaaaga ccccacctgt aggtttggca agctagctgc agtaacgcca ttttgcaagg
     4201 catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt acatgaaaat
     4261 agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg gcccggggcc
     4321 aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag atggtcccca
     4381 gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag gctcccccaa
     4441 ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt ctcgcttctg
     4501 ttcgcgcgct tctgcttccc gagctctana aaagagctca caacccctca ctcggcgcgc
     4561 cagtcctccg acagactgag tcgcccgggg ggcggccgca tgtcaagctc ttcctggctc
     4621 cttctcagcc ttgttgctgt aactgctgct cagtccacca ttgaggaaca ggccaagaca
     4681 tttttggaca agtttaacca cgaagccgaa gacctgttct atcaaagttc acttgcttct
     4741 tggaattata acaccaatat tactgaagag aatgtccaaa acatgaataa tgctggggac
     4801 aaatggtctg cctttttaaa ggaacagtcc acacttgccc aaatgtatcc actacaagaa
     4861 attcagaatc tcacagtcaa gcttcagctg caggctcttc agcaaaatgg gtcttcagtg
     4921 ctctcagaag acaagagcaa acggttgaac acaattctaa atacaatgag caccatctac
     4981 agtactggaa aagtttgtaa cccagataat ccacaagaat gcttattact tgaaccaggt
     5041 ttgaatgaaa taatggcaaa cagtttagac tacaatgaga ggctctgggc ttgggaaagc
     5101 tggagatctg aggtcggcaa gcagctgagg ccattatatg aagagtatgt ggtcttgaaa
     5161 aatgagatgg caagagcaaa tcattatgag gactatgggg attattggag aggagactat
     5221 gaagtaaatg gggtagatgg ctatgactac agccgcggcc agttgattga agatgtggaa
     5281 catacctttg aagagattaa accattatat gaacatcttc atgcctatgt gagggcaaag
     5341 ttgatgaatg cctatccttc ctatatcagt ccaattggat gcctccctgc tcatttgctt
     5401 ggtgatatgt ggggtagatt ttggacaaat ctgtactctt tgacagttcc ctttggacag
     5461 aaaccaaaca tagatgttac tgatgcaatg gtggaccagg cctgggatgc acagagaata
     5521 ttcaaggagg ccgagaagtt ctttgtatct gttggtcttc ctaatatgac tcaaggattc
     5581 tgggaaaatt ccatgctaac ggacccagga aatgttcaga aagcagtctg ccatcccaca
     5641 gcttgggacc tggggaaggg cgacttcagg atccttatgt gcacaaaggt gacaatggac
     5701 gacttcctga cagctcatca tgagatgggg catatccagt atgatatggc atatgctgca
     5761 caaccttttc tgctaagaaa tggagctaat gaaggattcc atgaagctgt tggggaaatc
     5821 atgtcacttt ctgcagccac acctaagcat ttaaaatcca ttggtcttct gtcacccgat
     5881 tttcaagaag acaatgaaac agaaataaac ttcctgctca aacaagcact cacgattgtt
     5941 gggactctgc catttactta catgttagag aagtggaggt ggatggtctt taaaggggaa
     6001 attcccaaag accagtggat gaaaaagtgg tgggagatga agcgagagat agttggggtg
     6061 gtggaacctg tgccccatga tgaaacatac tgtgaccccg catctctgtt ccatgtttct
     6121 aatgattact cattcattcg atattacaca aggacccttt accaattcca gtttcaagaa
     6181 gcactttgtc aagcagctaa acatgaaggc cctctgcaca aatgtgacat ctcaaactct
     6241 acagaagctg gacagaaact gttcaatatg ctgaggcttg gaaaatcaga accctggacc
     6301 ctagcattgg aaaatgttgt aggagcaaag aacatgaatg taaggccact gctcaactac
     6361 tttgagccct tatttacctg gctgaaagac cagaacaaga attcttttgt gggatggagt
     6421 accgactgga gtccatatgc agaccaaagc atcaaagtga ggataagcct aaaatcagct
     6481 cttggagata aagcatatga atggaacgac aatgaaatgt acctgttccg atcatctgtt
     6541 gcatatgcta tgaggcagta ctttttaaaa gtaaaaaatc agatgattct ttttggggag
     6601 gaggatgtgc gagtggctaa tttgaaacca agaatctcct ttaatttctt tgtcactgca
     6661 cctaaaaatg tgtctgatat cattcctaga actgaagttg aaaaggccat caggatgtcc
     6721 cggagccgta tcaatgatgc tttccgtctg aatgacaaca gcctagagtt tctggggata
     6781 cagccaacac ttggacctcc taaccagccc cctgtttcca tatggctgat tgtttttgga
     6841 gttgtgatgg gagtgatagt ggttggcatt gtcatcctga tcttcactgg gatcagagat
     6901 cggaagaaga aaaataaagc aagaagtgga gaaaatcctt atgcctccat cgatattagc
     6961 aaaggagaaa ataatccagg attccaaaac actgatgatg ttcagacctc cttttagctc
     7021 gaggttaacg aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag
     7081 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     7141 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     7201 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     7261 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     7321 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     7381 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     7441 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     7501 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     7561 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatgatt
     7621 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     7681 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     7741 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     7801 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     7861 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     7921 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     7981 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     8041 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     8101 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     8161 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     8221 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8281 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8341 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8401 ttcttctgaa tgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     8461 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     8521 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     8581 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     8641 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     8701 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     8761 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     8821 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8881 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     8941 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     9001 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     9061 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     9121 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     9181 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9241 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9301 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     9361 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     9421 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9481 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     9541 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9601 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9661 ttttttggag gcctaggctt ttgcgtcgag acgtacccaa ttcgccctat agtgagtcgt
     9721 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     9781 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     9841 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc
     9901 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     9961 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
    10021 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
    10081 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
    10141 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
    10201 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
    10261 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
    10321 attttaacaa aatattaacg tttacaattt cc
//
