LOCUS       Exported                8958 bp ds-DNA     circular SYN 07-APR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    MT136 pRRL SFFV-ACE2 (no IRES-puro)
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Caroline Goujon
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 28, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    330..992
                     /label=AmpR
                     /note="AmpR"
     misc_feature    1087..1769
                     /label=colE1 origin
                     /note="colE1 origin"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    2448..2628
                     /label=5'LTR (R U5)
                     /note="5'LTR (R U5)"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2671..2796
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2675..2812
                     /label=PSI
                     /note="PSI"
     misc_feature    3133..3140
                     /label=Mutated NotI
     misc_feature    3289..3523
                     /label=RRE
                     /note="RRE"
     misc_feature    3289..3522
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3901..4037
                     /label=cPPT-CTS
                     /note="cPPT-CTS"
     misc_feature    4018..4135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     4134..4167
                     /label=Primer 2
     promoter        4182..4589
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     4507..4524
                     /label=M90
     primer_bind     4592..4621
                     /label=oBB788
     gene            4600..7017
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /label=ACE2
                     /note="angiotensin I converting enzyme 2"
                     /db_xref="GeneID:59272"
                     /db_xref="HGNC:HGNC:13557"
                     /db_xref="MIM:300335"
     exon            4600..4785
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     sig_peptide     4600..4650
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="COORDINATES:same species:SignalP"
     mat_peptide     4651..6723
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /product="Processed angiotensin-converting enzyme 2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9BYF1.2)"
                     /experiment="experimental evidence, no additional details
                     recorded"
     exon            4786..4944
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            4945..5038
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            5039..5182
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     primer_bind     5100..5121
                     /label=oBB792
     exon            5183..5295
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            5296..5401
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            5402..5499
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            5500..5669
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            5670..5896
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     primer_bind     5697..5716
                     /label=oBB793
     exon            5897..6041
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            6042..6140
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            6141..6263
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            6264..6436
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     primer_bind     6270..6292
                     /label=oBB794
     exon            6437..6495
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            6496..6596
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            6597..6713
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     exon            6714..6908
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     misc_feature    6820..6882
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9BYF1.2); 
                     transmembrane region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     exon            6909..7017
                     /gene="ACE2"
                     /gene_synonym="ACEH"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(6990..7022)
                     /label=oBB789
     misc_feature    7024..7612
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    7026..7601
                     /label=WPRE
                     /note="WPRE"
     primer_bind     complement(7078..7097)
                     /label=shseq
     CDS             complement(7495..7506)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7699..7932
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7699..7751
                     /label=U3'
                     /note="U3'"
     misc_feature    7752..7932
                     /label=R U5
                     /note="R U5"
     polyA_signal    8004..8125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8144..8279
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8312..8330)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8340..8356)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      8501..8956
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggccccgaac
     2641 agggacctga aagcgaaagg gaaaccagag ctctctcgac gcaggactcg gcttgctgaa
     2701 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     2761 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     2821 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     2881 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2941 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     3001 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3061 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3121 accgcacagc aaggggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     3181 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     3241 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     3301 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcctcaatga cgctgacggt
     3361 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     3421 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     3481 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     3541 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     3601 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     4141 taatgaaaga ccccacctgt aggtttggca agctagctgc agtaacgcca ttttgcaagg
     4201 catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt acatgaaaat
     4261 agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg gcccggggcc
     4321 aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag atggtcccca
     4381 gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag gctcccccaa
     4441 ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt ctcgcttctg
     4501 ttcgcgcgct tctgcttccc gagctctana aaagagctca caacccctca ctcggcgcgc
     4561 cagtcctccg acagactgag tcgcccgggg ggcggccgca tgtcaagctc ttcctggctc
     4621 cttctcagcc ttgttgctgt aactgctgct cagtccacca ttgaggaaca ggccaagaca
     4681 tttttggaca agtttaacca cgaagccgaa gacctgttct atcaaagttc acttgcttct
     4741 tggaattata acaccaatat tactgaagag aatgtccaaa acatgaataa tgctggggac
     4801 aaatggtctg cctttttaaa ggaacagtcc acacttgccc aaatgtatcc actacaagaa
     4861 attcagaatc tcacagtcaa gcttcagctg caggctcttc agcaaaatgg gtcttcagtg
     4921 ctctcagaag acaagagcaa acggttgaac acaattctaa atacaatgag caccatctac
     4981 agtactggaa aagtttgtaa cccagataat ccacaagaat gcttattact tgaaccaggt
     5041 ttgaatgaaa taatggcaaa cagtttagac tacaatgaga ggctctgggc ttgggaaagc
     5101 tggagatctg aggtcggcaa gcagctgagg ccattatatg aagagtatgt ggtcttgaaa
     5161 aatgagatgg caagagcaaa tcattatgag gactatgggg attattggag aggagactat
     5221 gaagtaaatg gggtagatgg ctatgactac agccgcggcc agttgattga agatgtggaa
     5281 catacctttg aagagattaa accattatat gaacatcttc atgcctatgt gagggcaaag
     5341 ttgatgaatg cctatccttc ctatatcagt ccaattggat gcctccctgc tcatttgctt
     5401 ggtgatatgt ggggtagatt ttggacaaat ctgtactctt tgacagttcc ctttggacag
     5461 aaaccaaaca tagatgttac tgatgcaatg gtggaccagg cctgggatgc acagagaata
     5521 ttcaaggagg ccgagaagtt ctttgtatct gttggtcttc ctaatatgac tcaaggattc
     5581 tgggaaaatt ccatgctaac ggacccagga aatgttcaga aagcagtctg ccatcccaca
     5641 gcttgggacc tggggaaggg cgacttcagg atccttatgt gcacaaaggt gacaatggac
     5701 gacttcctga cagctcatca tgagatgggg catatccagt atgatatggc atatgctgca
     5761 caaccttttc tgctaagaaa tggagctaat gaaggattcc atgaagctgt tggggaaatc
     5821 atgtcacttt ctgcagccac acctaagcat ttaaaatcca ttggtcttct gtcacccgat
     5881 tttcaagaag acaatgaaac agaaataaac ttcctgctca aacaagcact cacgattgtt
     5941 gggactctgc catttactta catgttagag aagtggaggt ggatggtctt taaaggggaa
     6001 attcccaaag accagtggat gaaaaagtgg tgggagatga agcgagagat agttggggtg
     6061 gtggaacctg tgccccatga tgaaacatac tgtgaccccg catctctgtt ccatgtttct
     6121 aatgattact cattcattcg atattacaca aggacccttt accaattcca gtttcaagaa
     6181 gcactttgtc aagcagctaa acatgaaggc cctctgcaca aatgtgacat ctcaaactct
     6241 acagaagctg gacagaaact gttcaatatg ctgaggcttg gaaaatcaga accctggacc
     6301 ctagcattgg aaaatgttgt aggagcaaag aacatgaatg taaggccact gctcaactac
     6361 tttgagccct tatttacctg gctgaaagac cagaacaaga attcttttgt gggatggagt
     6421 accgactgga gtccatatgc agaccaaagc atcaaagtga ggataagcct aaaatcagct
     6481 cttggagata aagcatatga atggaacgac aatgaaatgt acctgttccg atcatctgtt
     6541 gcatatgcta tgaggcagta ctttttaaaa gtaaaaaatc agatgattct ttttggggag
     6601 gaggatgtgc gagtggctaa tttgaaacca agaatctcct ttaatttctt tgtcactgca
     6661 cctaaaaatg tgtctgatat cattcctaga actgaagttg aaaaggccat caggatgtcc
     6721 cggagccgta tcaatgatgc tttccgtctg aatgacaaca gcctagagtt tctggggata
     6781 cagccaacac ttggacctcc taaccagccc cctgtttcca tatggctgat tgtttttgga
     6841 gttgtgatgg gagtgatagt ggttggcatt gtcatcctga tcttcactgg gatcagagat
     6901 cggaagaaga aaaataaagc aagaagtgga gaaaatcctt atgcctccat cgatattagc
     6961 aaaggagaaa ataatccagg attccaaaac actgatgatg ttcagacctc cttttagctc
     7021 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7081 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7141 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7201 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7261 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7321 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7381 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     7441 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7501 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7561 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     7621 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7681 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7741 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7801 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7861 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7921 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     7981 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     8041 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8101 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     8161 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8221 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8281 aggcttttgc gtcgagacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     8341 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     8401 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     8461 ccttcccaac agttgcgcag cctgaatggc gaatggcgcg acgcgccctg tagcggcgca
     8521 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8581 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8641 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8701 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8761 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8821 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8881 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8941 ttaacgttta caatttcc
//
