LOCUS       Exported                9524 bp ds-DNA     circular SYN 15-APR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    MT130 pRRL SFFV-TMPRSS2-IRES-neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Caroline Goujon
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 28, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label=bla AmpR promoter
                     /label=bla promoter
                     /note="AmpR promoter"
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /label=bla CDS
                     /note="AmpR
                     confers resistance to ampicillin, carbenicillin, and 
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    330..992
                     /label=AmpR
                     /note="AmpR"
     misc_feature    1087..1769
                     /label=colE1 origin
                     /note="colE1 origin"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori rep origin
                     /note="ori
                     high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP bindin
                     /label=CAP binding site protein bind
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site
                     CAP binding activates transcription in the presence of 
                     cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="lac promoter
                     promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator prot
                     /label=lac operator protein bind
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator
                     The lac repressor binds to the lac operator to inhibit 
                     transcription in E. coli. This inhibition can be relieved 
                     by adding lactose or isopropyl-beta-D-thiogalactopyranoside
                     (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
     promoter        2174..2192
                     /label=T3 promoter
                     /note="T3 promoter
                     promoter for bacteriophage T3 RNA polymerase"
     misc_feature    2306..2311
                     /label=SphI, BbuI, PaeI
                     /note="Geneious type: restriction site
                     Recognition_pattern=GCATG^C
                     annotation_group=GCATGC"
     misc_feature    complement(2331..2341)
                     /label=BaeI
                     /note="Geneious type: restriction site
                     Recognition_pattern=(10/15)ACNNNNGTAYC(12/7)
                     annotation_group=ACNNNNGTAYC"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="5' LTR (truncated)
                     truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2675..2812
                     /label=PSI
                     /note="PSI"
     misc_feature    2821..2826
                     /label=NruI, Bsp68I, BtuMI, RruI
                     /note="Geneious type: restriction site
                     Recognition_pattern=TCG^CGA
                     annotation_group=TCGCGA"
     misc_feature    3133..3140
                     /label=Mutated NotI
                     /note="Mutated NotI"
     misc_feature    3152..3162
                     /label=EcoNI, BstENI, XagI
                     /note="Geneious type: restriction site
                     Recognition_pattern=CCTNN^NNNAGG
                     annotation_group=CCTNNNNNAGG"
     misc_feature    3175..3180
                     /label=MfeI, MunI
                     /note="Geneious type: restriction site
                     Recognition_pattern=C^AATTG
                     annotation_group=CAATTG"
     misc_feature    3289..3523
                     /label=RRE
                     /note="RRE"
     misc_feature    3901..4037
                     /label=cPPT-CTS
                     /note="cPPT-CTS"
     misc_feature    4136..4141
                     /label=ClaI, BanIII, Bsa29I, BseCI, BshVI, BspDI, Bsu1
                     /label=ClaI, BanIII, Bsa29I, BseCI, BshVI, BspDI, Bsu15I, 
                     BsuTUI
                     /note="Geneious type: restriction site
                     Recognition_pattern=AT^CGAT
                     annotation_group=ATCGAT"
     misc_feature    4172..4177
                     /label=BmtI, BspOI
                     /note="Geneious type: restriction site
                     Recognition_pattern=GCTAG^C
                     annotation_group=GCTAGC"
     promoter        4182..4589
                     /label=SFFV promoter
                     /note="SFFV promoter
                     spleen focus-forming virus long terminal repeat (LTR) 
                     promoter"
     primer_bind     4507..4524
                     /label=M90 fw
     primer_bind     4592..4614
                     /label=oBB790
     CDS             4600..6189
                     /codon_start=1
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /product="transmembrane protease serine 2 isoform 1"
                     /EC_number="3.4.21.-"
                     /label=TMPRSS2
                     /note="isoform 1 is encoded by transcript variant 1; 
                     epitheliasin; serine protease 10; transmembrane protease, 
                     serine 2"
                     /db_xref="CCDS:CCDS54486.1"
                     /db_xref="GeneID:7113"
                     /db_xref="HGNC:HGNC:11876"
                     /db_xref="MIM:602060"
                     /protein_id="NP_001128571.1"
                     /translation="MPPAPPGVESGCEERGAAGHIEHSRYLSLLDAVDNSKMALNSGSP
                     PAIGPYYENHGYQPENPYPAQPTVVPTVYEVHPAQYYPSPVPQYAPRVLTQASNPVVCT
                     QPKSPSGTVCTSKTKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTC
                     INPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPVCQDDWNENYGRAAC
                     RDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIAC
                     GVNLNSSRQSRIVGGESALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLN
                     NPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKLQKPLTFNDLVK
                     PVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLIT
                     PAMICAGFLQGNVDSCQGDSGGPLVTSKNNIWWLIGDTSWGSGCAKAYRPGVYGNVMVF
                     TDWIYRQMRADG"
     exon            4600..4654
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     exon            4655..4725
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     exon            4726..4948
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     exon            4949..5035
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     exon            5036..5155
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     primer_bind     5143..5163
                     /label=oBB795
     exon            5156..5282
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     exon            5283..5393
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     exon            5394..5437
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     exon            5438..5609
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     exon            5610..5785
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     exon            5786..5881
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     exon            5882..6024
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     exon            6025..6177
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(6167..6195)
                     /label=oBB791
     exon            6178..6189
                     /gene="TMPRSS2"
                     /gene_synonym="PP9284; PRSS10"
                     /inference="alignment:same species:2.1.0"
     primer_bind     6190..6212
                     /label=oCG552 KCL
     misc_feature    6191..6194
                     /label=Ligation
                     /label=ligation
     misc_feature    6216..6777
                     /label=EMCV IRES
                     /note="EMCV IRES"
     primer_bind     complement(6295..6313)
                     /label=M23
     CDS             6787..7581
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    7584..7589
                     /label=SalI
                     /note="Geneious type: restriction site
                     Recognition_pattern=G^TCGAC
                     annotation_group=GTCGAC"
     misc_feature    7590..8178
                     /label=WPRE
                     /note="WPRE
                     woodchuck hepatitis virus posttranscriptional regulatory 
                     element"
     LTR             8265..8498
                     /label=3' LTR (Delta-U3)
                     /note="3' LTR (Delta-U3)
                     self-inactivating 3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8570..8691
                     /label=SV40 poly(A) signal polyA signal
                     /note="SV40 poly(A) signal
                     SV40 polyadenylation signal"
     rep_origin      8710..8845
                     /label=SV40 ori rep origin
                     /note="SV40 ori
                     SV40 origin of replication"
     promoter        complement(8878..8896)
                     /label=T7 promoter
                     /note="T7 promoter
                     promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8906..8922)
                     /label=M13 fwd primer bind
                     /note="M13 fwd
                     common sequencing primer, one of multiple similar variants"
     rep_origin      9067..9522
                     /direction=RIGHT
                     /label=f1 ori rep origin
                     /note="f1 ori
                     f1 bacteriophage origin of replication; arrow indicates 
                     direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggccccgaac
     2641 agggacctga aagcgaaagg gaaaccagag ctctctcgac gcaggactcg gcttgctgaa
     2701 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     2761 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     2821 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     2881 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2941 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     3001 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3061 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3121 accgcacagc aaggggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     3181 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     3241 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     3301 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcctcaatga cgctgacggt
     3361 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     3421 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     3481 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     3541 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     3601 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     4141 taatgaaaga ccccacctgt aggtttggca agctagctgc agtaacgcca ttttgcaagg
     4201 catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt acatgaaaat
     4261 agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg gcccggggcc
     4321 aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag atggtcccca
     4381 gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag gctcccccaa
     4441 ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt ctcgcttctg
     4501 ttcgcgcgct tctgcttccc gagctctata aaagagctca caacccctca ctcggcgcgc
     4561 cagtcctccg acagactgag tcgcccgggg ggcggccgca tgccccctgc cccgcccgga
     4621 gttgaaagcg ggtgtgagga gcgcggcgcg gcaggtcata ttgaacattc cagataccta
     4681 tcattactcg atgctgttga taacagcaag atggctttga actcagggtc accaccagct
     4741 attggacctt actatgaaaa ccatggatac caaccggaaa acccctatcc cgcacagccc
     4801 actgtggtcc ccactgtcta cgaggtgcat ccggctcagt actacccgtc ccccgtgccc
     4861 cagtacgccc cgagggtcct gacgcaggct tccaaccctg tcgtctgcac gcagcccaaa
     4921 tccccatccg ggacagtgtg cacctcaaag actaagaaag cactgtgcat caccttgacc
     4981 ctggggacct tcctcgtggg agctgcgctg gccgctggcc tactctggaa gttcatgggc
     5041 agcaagtgct ccaactctgg gatagagtgc gactcctcag gtacctgcat caacccctct
     5101 aactggtgtg atggcgtgtc acactgcccc ggcggggagg acgagaatcg gtgtgttcgc
     5161 ctctacggac caaacttcat ccttcaggtg tactcatctc agaggaagtc ctggcaccct
     5221 gtgtgccaag acgactggaa cgagaactac gggcgggcgg cctgcaggga catgggctat
     5281 aagaataatt tttactctag ccaaggaata gtggatgaca gcggatccac cagctttatg
     5341 aaactgaaca caagtgccgg caatgtcgat atctataaaa aactgtacca cagtgatgcc
     5401 tgttcttcaa aagcagtggt ttctttacgc tgtatagcct gcggggtcaa cttgaactca
     5461 agccgccaga gcaggattgt gggcggtgag agcgcgctcc cgggggcctg gccctggcag
     5521 gtcagcctgc acgtccagaa cgtccacgtg tgcggaggct ccatcatcac ccccgagtgg
     5581 atcgtgacag ccgcccactg cgtggaaaaa cctcttaaca atccatggca ttggacggca
     5641 tttgcgggga ttttgagaca atctttcatg ttctatggag ccggatacca agtagaaaaa
     5701 gtgatttctc atccaaatta tgactccaag accaagaaca atgacattgc gctgatgaag
     5761 ctgcagaagc ctctgacttt caacgaccta gtgaaaccag tgtgtctgcc caacccaggc
     5821 atgatgctgc agccagaaca gctctgctgg atttccgggt ggggagccac cgaggagaaa
     5881 gggaagacct cagaagtgct gaacgctgcc aaggtgcttc tcattgagac acagagatgc
     5941 aacagcagat atgtctatga caacctgatc acaccagcca tgatctgtgc cggcttcctg
     6001 caggggaacg tcgattcttg ccagggtgac agtggagggc ctctggtcac ttcgaagaac
     6061 aatatctggt ggctgatagg ggatacaagc tggggttctg gctgtgccaa agcttacaga
     6121 ccaggagtgt acgggaatgt gatggtattc acggactgga tttatcgaca aatgagggca
     6181 gacggctaac tcgaggttaa cgaattccgc cccccccccc taacgttact ggccgaagcc
     6241 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     6301 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     6361 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     6421 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     6481 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     6541 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     6601 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     6661 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     6721 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     6781 acaaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     6841 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     6901 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     6961 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     7021 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     7081 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     7141 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     7201 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     7261 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     7321 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     7381 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     7441 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     7501 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     7561 cttcttgacg agttcttctg aatgtcgaca atcaacctct ggattacaaa atttgtgaaa
     7621 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7681 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7741 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7801 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7861 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7921 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7981 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     8041 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     8101 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     8161 tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa
     8221 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     8281 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8341 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8401 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8461 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8521 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     8581 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8641 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8701 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8761 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8821 tagtgaggag gcttttttgg aggcctaggc ttttgcgtcg agacgtaccc aattcgccct
     8881 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     8941 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     9001 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     9061 ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     9121 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     9181 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     9241 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     9301 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     9361 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     9421 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     9481 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcc
//
