LOCUS       pEE495                 10272 bp ds-DNA     circular     15-APR-2020
DEFINITION  .
ACCESSION   urn.local...1350434838000.701
KEYWORDS    .
SOURCE      
  ORGANISM   .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    918..1281
                     /locus_tag="TRV2 5' Sequence"
                     /label="TRV2 5' Sequence"
                     /ApEinfo_label="TRV2 5' Sequence"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(8104..8364)
                     /note="bom site from pBR322"
                     /X-Transferred_From="pCAMBIA1301 (modified); pCAMBIA1302;
                     pCAMBIA2300; pCAMBIA2301"
                     /X-Transferred_Similarity="100.00%"
                     /vntifkey=21
                     /modified_by="User"
                     /Transferred_From="pCAMBIA1301 (modified), pCAMBIA1302,
                     pCAMBIA2300, pCAMBIA2301"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="pDIRECT_21C, pDIRECT_21D, pDIRECT_22C,
                     pDIRECT_22D, pDIRECT_23C, pDIRECT_23D, pDIRECT_25H,
                     pDIRECT_25I, pDIRECT_26H, pDIRECT_26I"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="bom site from pBR322"
                     /label="bom site from pBR322"
                     /ApEinfo_label="bom site from pBR322"
                     /ApEinfo_fwdcolor="#66ffcc; #ff6666; #ff8000"
                     /ApEinfo_revcolor="#ff6666; #ffcc66; cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(6694..7694)
                     /note="pVS1-REP; replication origin from pVS1"
                     /X-Transferred_From="pCAMBIA1301 (modified); pCAMBIA1302;
                     pCAMBIA2300; pCAMBIA2301"
                     /X-Transferred_Similarity="100.00%"
                     /vntifkey=33
                     /Transferred_From="pCAMBIA1301 (modified), pCAMBIA1302,
                     pCAMBIA2300, pCAMBIA2301"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="pDIRECT_21C, pDIRECT_21D, pDIRECT_22C,
                     pDIRECT_22D, pDIRECT_23C, pDIRECT_23D, pDIRECT_25H,
                     pDIRECT_25I, pDIRECT_26H, pDIRECT_26I"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="pVS1 ori"
                     /label="pVS1 ori"
                     /ApEinfo_label="pVS1 ori"
                     /ApEinfo_fwdcolor="#66ffcc; pink"
                     /ApEinfo_revcolor="#66ffcc; pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5101..6100)
                     /note="STA region from pVS1 plasmid"
                     /X-Transferred_From="pCAMBIA1301 (modified); pCAMBIA1302;
                     pCAMBIA2300; pCAMBIA2301"
                     /X-Transferred_Similarity="100.00%"
                     /vntifkey=21
                     /Transferred_From="pCAMBIA1301 (modified), pCAMBIA1302,
                     pCAMBIA2300, pCAMBIA2301"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="pDIRECT_21C, pDIRECT_21D, pDIRECT_22C,
                     pDIRECT_22D, pDIRECT_23C, pDIRECT_23D, pDIRECT_25H,
                     pDIRECT_25I, pDIRECT_26H, pDIRECT_26I"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="STA region from pVS1"
                     /label="STA region from pVS1"
                     /ApEinfo_label="STA region from pVS1"
                     /ApEinfo_fwdcolor="#66ffcc; #ff6666; #ff8000"
                     /ApEinfo_revcolor="#ff6666; #ffcc66; cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     Viral_Elements  1282..1445
                     /created_by="User"
                     /locus_tag="TRV2 Viral Promoter"
                     /label="TRV2 Viral Promoter"
                     /ApEinfo_label="TRV2 Viral Promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2558..2656
                     /locus_tag="FT"
                     /label="FT"
                     /ApEinfo_label="FT"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3124..3222
                     /locus_tag="FT(1)"
                     /label="FT(1)"
                     /ApEinfo_label="FT"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2841..2939
                     /locus_tag="FT(2)"
                     /label="FT(2)"
                     /ApEinfo_label="FT"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     Viral_Elements  1478..2089
                     /created_by="User"
                     /locus_tag="Coat Protein"
                     /label="Coat Protein"
                     /ApEinfo_label="Coat Protein"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     Other           2259..2445
                     /vntifkey=21
                     /modified_by="User"
                     /locus_tag="PeBV sgPromoter"
                     /label="PeBV sgPromoter"
                     /ApEinfo_label="PeBV sgPromoter"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3233..3627
                     /vntifkey=21
                     /locus_tag="TRV 3' sequence"
                     /label="TRV 3' sequence"
                     /ApEinfo_label="TRV 3' sequence"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        149..917
                     /vntifkey=21
                     /modified_by="User"
                     /locus_tag="2x 35S"
                     /label="2x 35S"
                     /ApEinfo_label="2x 35S"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gRNA            3041..3116
                     /modified_by="User"
                     /modified_by="User"
                     /locus_tag="gRNA repeat"
                     /label="gRNA repeat"
                     /ApEinfo_label="gRNA repeat"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9075..9869
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gRNA            2475..2550
                     /modified_by="User"
                     /modified_by="User"
                     /locus_tag="gRNA repeat(1)"
                     /label="gRNA repeat(1)"
                     /ApEinfo_label="gRNA repeat"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    22..47
                     /locus_tag="T-DNA Border (L)"
                     /label="T-DNA Border (L)"
                     /ApEinfo_label="T-DNA Border (L)"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gRNA            2758..2833
                     /modified_by="User"
                     /modified_by="User"
                     /locus_tag="gRNA repeat(2)"
                     /label="gRNA repeat(2)"
                     /ApEinfo_label="gRNA repeat"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4033..4058
                     /locus_tag="T-DNA Border (R)"
                     /label="T-DNA Border (R)"
                     /ApEinfo_label="T-DNA Border (R)"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3730..3992
                     /locus_tag="Nos Terminator"
                     /label="Nos Terminator"
                     /ApEinfo_label="Nos Terminator"
                     /ApEinfo_fwdcolor="#a40000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1446..1477
                     /locus_tag="TRV2 5' Sequence(1)"
                     /label="TRV2 5' Sequence(1)"
                     /ApEinfo_label="TRV2 5' Sequence"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2090..2258
                     /locus_tag="TRV2 Sequence"
                     /label="TRV2 Sequence"
                     /ApEinfo_label="TRV2 Sequence"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3021..3040
                     /locus_tag="NbAG sgRNA2"
                     /label="NbAG sgRNA2"
                     /ApEinfo_label="NbAG sgRNA2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2455..2474
                     /locus_tag="NbAG sgRNA1"
                     /label="NbAG sgRNA1"
                     /ApEinfo_label="NbAG sgRNA1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2738..2757
                     /locus_tag="NbPDS3 gRNA"
                     /label="NbPDS3 gRNA"
                     /ApEinfo_label="NbPDS3 gRNA"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     tRNA            2657..2733
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="AACAAAGCACCAGTGGTCTAGTGGTAGAATAGTACCCTGCCACGGTACAG
                     ACCCGGGTTCGATTCCCGGCTGGTGCA"
                     /annotation_group="tRNA-Gly: 2,764 -> 2,840"
                     /locus_tag="tRNA-Gly"
                     /label="tRNA-Gly"
                     /ApEinfo_label="tRNA-Gly"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     tRNA            2940..3016
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="AACAAAGCACCAGTGGTCTAGTGGTAGAATAGTACCCTGCCACGGTACAG
                     ACCCGGGTTCGATTCCCGGCTGGTGCA"
                     /annotation_group="tRNA-Gly: 2,764 -> 2,840"
                     /locus_tag="tRNA-Gly(1)"
                     /label="tRNA-Gly(1)"
                     /ApEinfo_label="tRNA-Gly"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
       61 acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcc
      121 taggccacca tgttgggccc ggcgcgccaa gcttgcatgc ctgcaggtca acatggtgga
      181 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accagagggc
      241 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
      301 tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca
      361 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg
      421 acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      481 agtggattga tgtgatggtc aacatggtgg agcacgacac tctcgtctac tccaagaata
      541 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
      601 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
      661 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
      721 atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa
      781 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
      841 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
      901 catttcattt ggagaggata aaacattgca cctatggtgt tgccctggct ggggtatgtc
      961 agtgatcgca gtagaatgta ctaattgaca agttggagaa tacggtagaa cgtccttatc
     1021 caacacagcc tttatccctc tccctgacga ggtttttgtc agtgtaatat ttctttttga
     1081 actatccagc ttagtaccgt acgggaaagt gactggtgtg cttatctttg aaatgttact
     1141 ttgggtttcg gttctttagg ttagtaagaa agcacttgtc ttctcataca aaggaaaacc
     1201 tgagacgtat cgcttacgaa agtagcaatg aaagaaaggt ggtggtttta atcgctaccg
     1261 caaaaacgat ggggtcgttt taattaactt ctcctacgca agcgtctaaa cggacgttgg
     1321 ggttttgcta gtttctttag agaaaactag ctaagtcttt aatgttatca ttagagatgg
     1381 cataaatata atacttgtgt ctgctgataa gatcatttta atttggacga ttagacttgt
     1441 tgaactacag gttactgaat cacttgcgct aatcaacatg ggagatatgt acgatgaatc
     1501 atttgacaag tcgggcggtc ctgctgactt gatggacgat tcttgggtgg aatcagtttc
     1561 gtggaaagat ctgttgaaga agttacacag cataaaattt gcactacagt ctggtagaga
     1621 tgagatcact gggttactag cggcactgaa tagacagtgt ccttattcac catatgagca
     1681 gtttccagat aagaaggtgt atttcctttt agactcacgg gctaacagtg ctcttggtgt
     1741 gattcagaac gcttcagcgt tcaagagacg agctgatgag aagaatgcag tggcgggtgt
     1801 tacaaatatt cctgcgaatc caaacacaac ggttacgacg aaccaaggga gtactactac
     1861 taccaaggcg aacactggct cgactttgga agaagacttg tacacttatt acaaattcga
     1921 tgatgcctct acagctttcc acaaatctct aacttcgtta gagaacatgg agttgaagag
     1981 ttattaccga aggaactttg agaaagtatt cgggattaag tttggtggag cagctgctag
     2041 ttcatctgca ccgcctccag cgagtggagg tccgatacgt cctaatccct agggatttaa
     2101 ggacgtgaac tctgttgaga tctctgtgaa attcagaggg tgggtgatac catattcact
     2161 gatgccatta gcgacatcta aatagggcta attgtgacta atttgaggga atttccttta
     2221 ccattgacgt cagtgtcgtt ggtagcattt gagtttcgga gcatcttgtt ctggggtttc
     2281 acactatctt tagagaaagt gttaagttaa ttaagttatc ttaattaaga gcataattat
     2341 actgatttgt ctctcgttga tagagtctat cattctgtta ctaaaaattt gacaactcgg
     2401 tttgctgacc tactggttac tgtatcactt acccgagtta acgagtctag AAACGTGTGA
     2461 AAGAAACAAT TGAGgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     2521 caacttgaaa aagtggcacc gagtcggtgc TCCCtagtct ataaatataa gagaccctct
     2581 tatagtaagc agagttgttg gagacgttct tgatccgttt aatagatcaa tcactctaaa
     2641 ggttacttat ggccaaaaca aagcaccagt ggtctagtgg tagaatagta ccctgccacg
     2701 gtacagaccc gggttcgatt cccggctggt gcaGTGCTTG GTAGTAGCGA CTCCATGgtt
     2761 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     2821 accgagtcgg tgcTCCCtag tctataaata taagagaccc tcttatagta agcagagttg
     2881 ttggagacgt tcttgatccg tttaatagat caatcactct aaaggttact tatggccaaa
     2941 acaaagcacc agtggtctag tggtagaata gtaccctgcc acggtacaga cccgggttcg
     3001 attcccggct ggtgcaCAGG TTGATTGTCT TCTCAAGCAG gttttagagc tagaaatagc
     3061 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcTCCC
     3121 tagtctataa atataagaga ccctcttata gtaagcagag ttgttggaga cgttcttgat
     3181 ccgtttaata gatcaatcac tctaaaggtt acttatggcc aaAGTGccgg gcatgtcccg
     3241 aagacattaa actacggttc tttaagtaga tccgtgtctg aagttttagg ttcaatttaa
     3301 acctacgaga ttgacattct cgactgatct tgattgatcg gtaagtcttt tgtaatttaa
     3361 ttttcttttt gattttattt taaattgtta tctgtttctg tgtatagact gtttgagatc
     3421 ggcgtttggc cgactcattg tcttaccata ggggaacgga ctttgtttgt gttgttattt
     3481 tatttgtatt ttattaaaat tctcaacgat ctgaaaaagc ctcgcggcta agagattgtt
     3541 ggggggtgag taagtacttt taaagtgatg atggttacaa aggcaaaagg ggtaaaaccc
     3601 ctcgcctacg taagcgttat tacgcccgtc tgtacttata tcagtacact gacgagtccc
     3661 taaaggacga aacgggagaa cgctagccac caccaccacc accacgtgtg aattacaggt
     3721 gaccagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     3781 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     3841 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     3901 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     3961 atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta aactatcagt gtttgacagg
     4021 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataaTcg gatatttaaa
     4081 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     4141 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
     4201 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
     4261 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
     4321 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
     4381 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
     4441 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
     4501 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
     4561 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
     4621 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
     4681 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
     4741 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
     4801 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
     4861 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
     4921 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
     4981 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
     5041 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
     5101 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
     5161 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
     5221 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
     5281 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
     5341 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
     5401 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
     5461 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
     5521 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
     5581 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
     5641 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
     5701 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
     5761 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
     5821 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
     5881 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
     5941 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
     6001 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
     6061 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
     6121 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
     6181 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
     6241 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
     6301 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgCct gccggccctg
     6361 caatggcact ggaaccccca agcccgagga atcggcgtga Gcggtcgcaa accatccggc
     6421 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
     6481 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
     6541 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
     6601 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
     6661 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
     6721 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
     6781 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
     6841 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
     6901 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
     6961 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
     7021 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
     7081 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
     7141 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
     7201 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
     7261 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
     7321 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
     7381 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
     7441 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
     7501 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac
     7561 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
     7621 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
     7681 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
     7741 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
     7801 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
     7861 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
     7921 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     7981 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     8041 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     8101 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     8161 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     8221 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8281 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8341 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8401 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8461 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8521 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8581 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8641 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8701 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8761 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8821 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8881 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8941 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9001 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9061 atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
     9121 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
     9181 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
     9241 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
     9301 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
     9361 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
     9421 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
     9481 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
     9541 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
     9601 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
     9661 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
     9721 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
     9781 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
     9841 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
     9901 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
     9961 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
    10021 acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc
    10081 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
    10141 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
    10201 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
    10261 gagctgccgg tc    
//
