LOCUS       pTP264_H14-Sp-br        5243 bp    DNA     circular     17-APR-2020
vom pQE80 die NcoI Schnittstelle 861 gegen BspHI mutiert; Mutagenes Oligo:
TTCTTCGCCCCCGTTTTCAtCATGaGCAAATATTATACGCAAGGC

Expressionskassette von pQE60 als AatII/ XbaI Fragment transferiert.
FEATURES             Location/Qualifiers
     misc_feature    complement(2668..2765)
                     /note="rrnB T1 trx termination region"
     misc_feature    complement(2813..3914)
                     /note="lacI E.coli genomic sequence"
     misc_feature    4063..4165
                     /note="part of H-strand Y effector site"
     misc_feature    1640..2455
                     /note="KanR"
     misc_feature    25..30
                     /note="-35 box"
     misc_feature    7..23
                     /note="-43 AT rich region"
     misc_feature    48..53
                     /note="-10 box"
     misc_feature    60..60
                     /note="transcription start"
     misc_feature    62..80
                     /note="lacO"
     misc_feature    30..48
                     /note="lacO"
     misc_feature    96..106
                     /note="RBS"
     misc_feature    1487..1581
                     /note="lambda t0 trx termination region"
     misc_feature    4532..5120
                     /note="ColE1 origin"
     misc_feature    4533..4533
                     /note="replication origin"
     misc_feature    265..492
                     /note="bdNEDD8 from Brachypodium distachyon"
     misc_feature    115..192
                     /note="His14-TAG"
     misc_feature    502..1466
                     /note="Biotin ligase BirA from E. coli"
     source          1..5243
                     /dnas_title="pTP264_H14-Sp-brNEDD8-BirA"
ORIGIN      
        1 CTCGAGAAAT CATAAAAAAT TTATTTGCTT TGTGAGCGGA TAACAATTAT AATAGATTCA
       61 ATTGTGAGCG GATAACAATT TCACACAGAA TTCATTAAaG AGGaGAAATT AACcATGAGC
      121 AAGCATCACC ATCATTCAGG CCATCACCAt aCCGGaCACC ACCATCATTC aGGCAGTCAT
      181 CACCAtaCCG GCggatcatc cggaGGTATg agctCAGGAT CTAACGGATC TACGGGTTCT
      241 GGTACTGGTA ATAGCGGGAC CGGAACCATG ATTAAAGTAA AGACTCTGAC CGGCAAGGAA
      301 ATTGAGATCG ACATCGAACC GACCGACACC ATCGATCGTA TCAAAGAACG TGTGGAGGAA
      361 AAAGAAGGCA TTCCTCCGGT TCAGCAGCGT CTCATTTACG CGGGTAAACA GCTTGCAGAT
      421 GACAAAACCG CCAAAGATTA CAATATCGAG GGCGGTAGCG TACTGCACCT TGTTCTGGCC
      481 CTGCGTGGTG GCGCCGGTAC Catgaaggat aacaccgtgc cactgaaatt gattgccctg
      541 ttagcgaacg gtgaatttca ctctggcgag cagttgggtg aaacgctggg aatgagccgg
      601 gcggctatta ataaacacat tcagacactg cgtgactggg gcgttgatgt ctttaccgtt
      661 ccgggtaaag gatacagcct gcctgagcct atccagttac ttaatgctaa acagatattg
      721 ggtcagctgg atggcggtag tgtagccgtg ctgccagtga ttgactccac gaatcagtac
      781 cttcttgatc gtatcggaga gcttaaatcg ggcgatgctt gcattgcaga ataccagcag
      841 gctggccgtg gtcgccgggg tcggaaatgg ttttcgcctt ttggcgcaaa cttatatttg
      901 tcgatgttct ggcgtctgga acaaggcccg gcggcggcga ttggtttaag tctggttatc
      961 ggtatcgtga tggcggaagt attacgcaag ctgggtgcag ataaagttcg tgttaaatgg
     1021 cctaatgacc tctatctgca ggatcgcaag ctggcaggca ttctggtgga gctgactggc
     1081 aaaactggcg atgcggcgca aatagtcatt ggagccggga tcaacatggc aatgcgccgt
     1141 gttgaagaga gtgtcgttaa tcaggggtgg atcacgctgc aggaagcggg gatcaatctc
     1201 gatcgtaata cgttggcggc catgctaata cgtgaattac gtgctgcgtt ggaactcttc
     1261 gaacaagaag gattggcacc ttatctgtcg cgctgggaaa agctggataa ttttattaat
     1321 cgcccagtga aacttatcat tggtgataaa gaaatatttg gcatttcacg cggaatagac
     1381 aaacaggggg ctttattact tgagcaggat ggaataataa aaccctggat gggcggtgaa
     1441 atatccctgc gtagtgccga aaaataAGCT TAATTAGCTG AGCTTGGACT CCTGTTGATA
     1501 GATCCAGTAA TGACCTCAGA ACTCCATCTG GATTTGTTCA GAACGCTCGG TTGCCGCCGG
     1561 GCGTTTTTTA TTGGTGAGAA TCCAAGctag ccatgaaaat aaactgtctg cttacataaa
     1621 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct ctaggccgcg
     1681 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     1741 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct
     1801 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     1861 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     1921 ttggttactc acgactgcga tccccggcaa aacagcattc caggtattag aagaatatcc
     1981 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     2041 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     2101 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     2161 tgttgaacaa gtctggaaag aaatgcacaa acttttgcca ttctcaccgg attcagtcgt
     2221 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     2281 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     2341 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     2401 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaagaatt
     2461 aattcatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     2521 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     2581 ACTGCGATGA GTGGCAGGGC GGGGCGTAAT TTTTTTAAGG CAGTTATTGG TGCCCTTAAA
     2641 CGCCTGGGGT AATGACTCTC TAGCTTGAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA
     2701 GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT
     2761 CCGCCGCTCT AGATTACGTG CAGTCGATGA TAAGCTGTCA AACATGAGAA TTGTGCCTAA
     2821 TGAGTGAGCT AACTTACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC
     2881 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT
     2941 GGGgGCCAGG GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC
     3001 CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA
     3061 ATCCTGTTTG ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG TATCGTCGTA
     3121 TCCCACTACC GAGATATCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC
     3181 GCCCAGCGCC ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG
     3241 CATTTGCATG GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC GTTCCGCTAT
     3301 CGGCTGAATT TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA
     3361 GACAGAACTT AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG CGACCAGATG
     3421 CTCCACGCCC AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG
     3481 GTCAGAGACA TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC
     3541 ATCCTGGTCA TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG CGAGAAGATT
     3601 GTGCACCGCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT
     3661 GGCACCCAGT TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG GCGCGTGCAG
     3721 GGCCAGACTG GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC
     3781 CACGCGGTTG GGAATGTAAT TCAGCTCCGC CATCGCCGCT TCCACTTTTT CCCGCGTTTT
     3841 CGCAGAAACG TGGCTGGCCT GGTTCACCAC GCGGGAAACG GTCTGATAAG AGACACCGGC
     3901 ATACTCTGCG ACATCGTATA ACGTTACTGG TTTCACATTC ACCACCCTGA ATTGACTCTC
     3961 TTCCGGGCGC TATCATGCCA TACCGCGAAA GGTTTTGCAC CATTCGATGG TGTCGGAATT
     4021 TCGGGCAGCG TTGGGTCCTG GCCACGGGTG CGCATGATCT AGAGCTGCCT CGCGCGTTTC
     4081 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG
     4141 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT
     4201 CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATACTGG CTTAACTATG
     4261 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT
     4321 GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC
     4381 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT
     4441 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA
     4501 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC
     4561 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC
     4621 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
     4681 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA
     4741 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG
     4801 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC
     4861 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG
     4921 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT
     4981 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
     5041 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
     5101 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT
     5161 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGACATT AACCTATAAA AATAGGCGTA
     5221 TCACGAGGCC CTTTCGTCTT CAC
//

