LOCUS pME18S_FLAG_rho_ 4326 bp ds-DNA linear 08-MAY-2020 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments= COMMENT SerialCloner_Ends=0,0,,0, COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers rep_origin complement(2557..3185) /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True /label=ColE1 origin /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(3337..3996) /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True /label=AmpR /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal complement(2170..2361) /SerialCloner_Color=&hFFC0CB /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True /label=SV40 late polyA /ApEinfo_fwdcolor="#ff3eee" /ApEinfo_revcolor="#ff3eee" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 151..347 /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True /label=SV40 early promoter /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" Promoter complement(4236..4264) /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True /label=Amp prom /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 198..275 /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True /label=SV40 ORI /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 803..826 /SerialCloner_Color=&h008000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True /label=Flag tag /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 899..2140 /SerialCloner_Color=&hFF0000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=hb2AR /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 833..892 /label=rho tag /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctccgat 361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctct gaaaaaccag aaagttaact 661 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg caaatcaaag 721 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc 781 tctaaaagct gcggaattcA TGGACTACAA GGACGACGAC GACAAGGAAT TCATGAACGG 841 GACCGAGGGC CCAAACTTCT ACGTGCCTTT CTCCAACAAG ACGGGCGTGG TGCTCGAGat 901 ggggcaaccc gggaacggca gcgccttctt gctggcaccc aatggaagcc atgcgccgga 961 ccacgacgtc acgcaggaaa gggacgaggt gtgggtggtg ggcatgggca tcgtcatgtc 1021 tctcatcgtc ctggccatcg tgtttggcaa tgtgctggtc atcacagcca ttgccaagtt 1081 cgagcgtctg cagacggtca ccaactactt catcacttca ctggcctgtg ctgatctggt 1141 catgggcctg gcagtggtgc cctttggggc cgcccatatt cttatgaaaa tgtggacttt 1201 tggcaacttc tggtgcgagt tttggacttc cattgatgtg ctgtgcgtca cggccagcat 1261 tgagaccctg tgcgtgatcg cagtggatcg ctactttgcc attacttcac ctttcaagta 1321 ccagagcctg ctgaccaaga ataaggcccg ggtgatcatt ctgatggtgt ggattgtgtc 1381 aggccttacc tccttcttgc ccattcagat gcactggtac cgggccaccc accaggaagc 1441 catcaactgc tatgccaatg agacctgctg tgacttcttc acgaaccaag cctatgccat 1501 tgcctcttcc atcgtgtcct tctacgttcc cctggtgatc atggtcttcg tctactccag 1561 ggtctttcag gaggccaaaa ggcagctcca gaagattgac aaatctgagg gccgcttcca 1621 tgtccagaac cttagccagg tggagcagga tgggcggacg gggcatggac tccgcagatc 1681 ttccaagttc tgcttgaagg agcacaaagc cctcaagacg ttaggcatca tcatgggcac 1741 tttcaccctc tgctggctgc ccttcttcat cgttaacatt gtgcatgtga tccaggataa 1801 cctcatccgt aaggaagttt acatcctcct aaattggata ggctatgtca attctggttt 1861 caatcccctt atctactgcc ggagcccaga tttcaggatt gccttccagg agcttctgtg 1921 cctgcgcagg tcttctttga aggcctatgg gaatggctac tccagcaacg gcaacacagg 1981 ggagcagagt ggatatcacg tggaacagga gaaagaaaat aaactgctgt gtgaagacct 2041 cccaggcacg gaagactttg tgggccatca aggtactgtg cctagcgata acattgattc 2101 acaagggagg aattgtagta caaatgactc actgctgtaa CCGCGGgcgg ccgctagact 2161 agtctagaga aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 2221 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 2281 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 2341 caatgtatct tatcatgtct ggatccccgg gtaccgagct cgaattaatt cctcttccgc 2401 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 2461 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 2521 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 2581 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 2641 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 2701 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 2761 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 2821 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 2881 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 2941 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 3001 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 3061 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 3121 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 3181 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 3241 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 3301 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 3361 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 3421 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 3481 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 3541 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 3601 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 3661 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 3721 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 3781 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 3841 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 3901 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 3961 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 4021 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 4081 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 4141 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 4201 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 4261 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 4321 acctgc //